{"currentpage":1,"firstResult":0,"maxresult":10,"pagecode":5,"pageindex":{"endPagecode":5,"startPagecode":1},"records":[{"abstractinfo":"利用静电自组装技术和通过改变溶液的pH值与组装循环数在云母基底上制备了壳聚糖/<span style=\"color:red\">血红蛋白</span>复合膜,并运用原子力显微镜(AFM)和红外光谱对其结构进行了表征.结果表明,在以去离子水为溶剂的<span style=\"color:red\">血红蛋白</span>溶液与pH为5.5的壳聚糖醋酸溶液复合制备的三循环膜结构紧密,壳聚糖与<span style=\"color:red\">血红蛋白</span>相互作用能较好地达到平衡.体现了壳聚糖与<span style=\"color:red\">血红蛋白</span>之间良好的生物相容性,为临床应用壳聚糖对<span style=\"color:red\">血红蛋白</span>进行修饰来改进其作为血液替代品的缺陷方面作了一些基础性的探讨.","authors":[{"authorName":"马豫峰","id":"8e2379c9-722b-4606-88c0-a3117f18eeab","originalAuthorName":"马豫峰"},{"authorName":"蔡继业","id":"201dd03a-ed13-4bc2-80ce-72d2632311d4","originalAuthorName":"蔡继业"},{"authorName":"杨培慧","id":"279ea2d1-7229-4dcb-8e80-5a9e0307b2cb","originalAuthorName":"杨培慧"},{"authorName":"陈勇","id":"c989c763-eccb-4a53-912f-ef28d057ba84","originalAuthorName":"陈勇"}],"doi":"","fpage":"272","id":"84384347-1770-4772-90aa-04ec3557bb99","issue":"1","journal":{"abbrevTitle":"GFZCLKXYGC","coverImgSrc":"journal/img/cover/GFZCLKXYGC.jpg","id":"31","issnPpub":"1000-7555","publisherId":"GFZCLKXYGC","title":"高分子材料科学与工程"},"keywords":[{"id":"e1d0e3ec-8262-403d-b5af-7fb66203f751","keyword":"壳聚糖","originalKeyword":"壳聚糖"},{"id":"b15cd28f-2fc6-407a-bd3f-c2b16a771038","keyword":"<span style=\"color:red\">血红蛋白</span>","originalKeyword":"血红蛋白"},{"id":"cb33bf0b-ccbe-46a5-8859-d6ecefcbc048","keyword":"自组装复合膜","originalKeyword":"自组装复合膜"},{"id":"550c5e0c-b09a-45da-909b-86d4cfc553c5","keyword":"原子力显微镜","originalKeyword":"原子力显微镜"}],"language":"zh","publisherId":"gfzclkxygc200501068","title":"壳聚糖自组装复合修饰<span style=\"color:red\">血红蛋白</span>的研究","volume":"21","year":"2005"},{"abstractinfo":"将纤维素滤纸进行碱处理及环氧活化、偶联亚氨基二乙酸、固定化铜离子等处理,并将其装入自制的色谱柱管,制得固定化铜离子亲和膜色谱柱.该柱可用于吸附<span style=\"color:red\">血红蛋白</span>(hemoglobin,Hb),吸附率可达到90% 以上.考察了上样量、pH值、温度、上样速度等因素对固定化铜离子亲和膜吸附Hb的影响.实验结果表明,固定化铜离子亲和膜色谱柱吸附<span style=\"color:red\">血红蛋白</span>的最佳条件为:室温下实验,缓冲体系的pH值控制在6～8,上样速度0.5～1.0 mL/min,上样量为3.16～7.90 mg/g.","authors":[{"authorName":"秦晓蓉","id":"d07e5027-2af4-4ba1-a00c-c4b5256d5691","originalAuthorName":"秦晓蓉"},{"authorName":"伍林","id":"742f91a0-0584-45f3-94c4-67c2b8bd9bfe","originalAuthorName":"伍林"},{"authorName":"易德莲","id":"4637d569-e49a-4480-ac0d-ffef7f56f439","originalAuthorName":"易德莲"},{"authorName":"胡雷","id":"31dc9a81-45f5-4f54-ae7d-dd493439a06d","originalAuthorName":"胡雷"},{"authorName":"曹淑超","id":"171186d8-ec75-44c1-9f5c-25f9c48d5e78","originalAuthorName":"曹淑超"}],"doi":"10.3321/j.issn:1000-8713.2005.03.009","fpage":"255","id":"e3157f43-703b-40db-ab89-619495226719","issue":"3","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"18cf7bad-5320-4077-98f1-bf48e5b7360f","keyword":"亲和膜","originalKeyword":"亲和膜"},{"id":"81b7bb83-ae1f-498a-a49a-694595b61fbc","keyword":"亲和色谱","originalKeyword":"亲和色谱"},{"id":"10ced7c0-e599-4a23-a050-d2340f769093","keyword":"固定化铜离子","originalKeyword":"固定化铜离子"},{"id":"17328028-ff4a-4aee-8801-a1d41e39c0d2","keyword":"<span style=\"color:red\">血红蛋白</span>","originalKeyword":"血红蛋白"},{"id":"c1719d73-ef33-4092-8e92-0b4925ce5cbe","keyword":"吸附","originalKeyword":"吸附"}],"language":"zh","publisherId":"sp200503009","title":"固定化铜离子亲和膜色谱柱吸附<span style=\"color:red\">血红蛋白</span>的研究","volume":"23","year":"2005"},{"abstractinfo":"H_2O_2在pH 9.8的NH_2·H_2O-NH_4Cl介质中产生的羟自由基可以迅速氧化结晶紫使其褪色,而<span style=\"color:red\">血红蛋白</span>模拟酶对褪色反应具有催化作用,据此建立了一种以结晶紫为指示剂的过氧化氢-结晶紫-<span style=\"color:red\">血红蛋白</span>酶催化反应体系测定痕量H_2O_2的新方法.研究了pH值,结晶紫,<span style=\"color:red\">血红蛋白</span>用量等因素对体系的影响.对雨水中Zn~(2+),Cu~(2+),Mg~(2+),Ca~(2+),Fe~(3+),Al~(2+)等常见离子进行了干扰试验.结果表明,除Fe~(3+)的允许量较小外,其他离子的允许量都较大;但是Fe~(3+)的干扰可以通过加入过量的柠檬酸消除.在最佳实验条件下,测定H~(2+)O~(2+)的线性范围为1.0×10~(-7)～3.0×10~(-5)mol/L,检出限为1.2×10~(-8)mol/L.该方法可用于测定雨水及消毒水中H_20_2的含量,结果与荧光法测定结果一致.","authors":[{"authorName":"陈亚红","id":"b7853347-60ac-4da6-8a5b-f70ba3b95674","originalAuthorName":"陈亚红"},{"authorName":"田丰收","id":"fad5848f-172e-4c31-be4a-fca760348e33","originalAuthorName":"田丰收"},{"authorName":"韩俊","id":"901e9acf-4fc7-4fec-b1ef-b403674e8576","originalAuthorName":"韩俊"}],"doi":"10.3969/j.issn.1000-7571.2010.02.010","fpage":"47","id":"ac3b2886-ba72-46e2-b001-07a8b402042d","issue":"2","journal":{"abbrevTitle":"YJFX","coverImgSrc":"journal/img/cover/YJFX.jpg","id":"71","issnPpub":"1000-7571","publisherId":"YJFX","title":"冶金分析   "},"keywords":[{"id":"edd8ccd6-6434-41b8-b753-efdd16c596ba","keyword":"<span style=\"color:red\">血红蛋白</span>","originalKeyword":"血红蛋白"},{"id":"b9f19fd9-1765-4d5b-a0f2-5c865c1c0704","keyword":"催化光度法","originalKeyword":"催化光度法"},{"id":"f31f1d2d-5d14-47b2-ad9e-b95c532096be","keyword":"过氧化氢","originalKeyword":"过氧化氢"}],"language":"zh","publisherId":"yjfx201002010","title":"<span style=\"color:red\">血红蛋白</span>模拟酶催化光度法测定过氧化氢","volume":"30","year":"2010"},{"abstractinfo":"采用电聚合法在玻碳电极(GCE)表面得到导电性能良好的聚L-谷氨酸(PGA)薄膜,通过共价键合法将<span style=\"color:red\">血红蛋白</span>(Hb)固定于电极表面得到稳定且具有催化活性的Hb/PGA/GCE修饰电极,将其用于对苯二胺(PPD)的可逆氧化. 修饰电极交流阻抗及<span style=\"color:red\">血红蛋白</span>直接电化学实验表明,<span style=\"color:red\">血红蛋白</span>成功地固定于电极表面,保持良好的电催化活性,能有效催化H2O2的还原. PPD在电极上表现为受吸附控制的准可逆氧化还原反应,Ip,a/Ip,c约为1.02,电极没有明显的钝化现象. 氧化还原峰电流与PPD的浓度均呈良好的线性关系,Ip,a(μA)=3.124+0.705cPPD(mmol/L)(r=0.9973). H2O2的存在使PPD氧化还原峰型更对称,可逆性更好,表明体系中PPD氧化与过氧化酶催化途径一致.","authors":[{"authorName":"李敏","id":"2c09e9f4-19ee-4a0f-890b-04daeeac1e8d","originalAuthorName":"李敏"},{"authorName":"杨昌英","id":"f3d46e0b-a2ba-43e2-8c2d-c8b8ed362875","originalAuthorName":"杨昌英"},{"authorName":"周文凯","id":"5d749c13-e38a-4de1-9759-a0327acdb5a9","originalAuthorName":"周文凯"},{"authorName":"朱敏","id":"5f36c3ea-fc6e-4865-a757-716dcbe72810","originalAuthorName":"朱敏"}],"doi":"10.3724/SP.J.1095.2010.90783","fpage":"1093","id":"bb6c92dc-3c18-4671-b37e-3f6a70ebfbc7","issue":"9","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"f8a690d7-2ee3-44f8-9d75-403bebf578a1","keyword":"<span style=\"color:red\">血红蛋白</span>","originalKeyword":"血红蛋白"},{"id":"14ff2b82-4126-498c-84f5-b8a37f100675","keyword":"聚L-谷氨酸","originalKeyword":"聚L-谷氨酸"},{"id":"8e691ce0-92b2-4358-9710-6a6c6a973af4","keyword":"对苯二胺","originalKeyword":"对苯二胺"},{"id":"acca0346-ce2f-4f82-8d9a-117736287d67","keyword":"可逆氧化","originalKeyword":"可逆氧化"}],"language":"zh","publisherId":"yyhx201009021","title":"对苯二胺在<span style=\"color:red\">血红蛋白</span>/聚L-谷氨酸/玻碳修饰电极上的可逆氧化","volume":"27","year":"2010"},{"abstractinfo":"为了从猪血中分离纯化高纯度的猪<span style=\"color:red\">血红蛋白</span>,建立了通过超滤、DEAE-Sepharose Fast Flow离子交换色谱和Sephadex G-75凝胶排阻色谱三步法制备高纯度猪<span style=\"color:red\">血红蛋白</span>的方法,并通过十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、高效凝胶排阻色谱和反相高效液相色谱方法,对纯化后的猪<span style=\"color:red\">血红蛋白</span>进行了鉴定.经三步分离纯化后,猪<span style=\"color:red\">血红蛋白</span>的纯度大于99% ,含量为1.328 g/L.","authors":[{"authorName":"周勃","id":"cf978955-db4f-4669-a76c-3aa208d434b2","originalAuthorName":"周勃"},{"authorName":"边六交","id":"c2d55151-b9dd-4e4e-bca1-245f4ef02ae9","originalAuthorName":"边六交"}],"doi":"10.3321/j.issn:1000-8713.2008.03.025","fpage":"384","id":"ce5d1350-73f5-4aee-b748-37baac4274e3","issue":"3","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"13f0dc3e-b285-498c-adb3-9a2e9cf23c0f","keyword":"超滤","originalKeyword":"超滤"},{"id":"15da9ea1-e6c0-4df0-9472-91ad252efdb6","keyword":"阴离子交换色谱","originalKeyword":"阴离子交换色谱"},{"id":"96efcbd4-fbe4-4456-8fb4-cb45db171893","keyword":"凝胶排阻色谱","originalKeyword":"凝胶排阻色谱"},{"id":"7844cd34-9f8f-4092-8ac0-8ea1d6d6cb1c","keyword":"猪<span style=\"color:red\">血红蛋白</span>","originalKeyword":"猪血红蛋白"},{"id":"a4ba9447-c271-4726-b7ff-bc07efece832","keyword":"分离","originalKeyword":"分离"},{"id":"57d9d4e0-8537-496c-8785-b22d1eca4f48","keyword":"纯化","originalKeyword":"纯化"}],"language":"zh","publisherId":"sp200803025","title":"从猪血中分离纯化高纯度的猪<span style=\"color:red\">血红蛋白</span>","volume":"26","year":"2008"},{"abstractinfo":"利用UV-Vis吸收光谱和荧光光谱研究了在生理pH值条件下竹红菌甲素(HA)与<span style=\"color:red\">血红蛋白</span>的相互作用. UV-Vis吸收光谱的研究发现,HA的存在使<span style=\"color:red\">血红蛋白</span>分子中氨基酸残基的吸收峰强度降低,峰位红移,表明HA与<span style=\"color:red\">血红蛋白</span>分子中的氨基酸残基形成氢键. 同步荧光光谱的结果表明,HA与<span style=\"color:red\">血红</span>素分子中不同氨基酸残基的作用强度不同,HA对色氨酸残基和酪氨酸残基的荧光猝灭动力学常数分别为5.5×1012和1.7×1012 L/mol,表明HA与色氨酸残基之间的相互作用强于与酪氨酸残基.","authors":[{"authorName":"冯玉英","id":"b61d99a0-713f-49b2-9857-ea4d072dbf79","originalAuthorName":"冯玉英"},{"authorName":"吴晓红","id":"efc2f98a-9c40-4584-85e0-27b6af21769e","originalAuthorName":"吴晓红"},{"authorName":"周家宏","id":"60af7a84-3107-4f99-82c9-5c6b2e4b2bf1","originalAuthorName":"周家宏"},{"authorName":"顾晓天","id":"f6f45fcb-bb5a-4aba-a897-610e3dc9e52a","originalAuthorName":"顾晓天"},{"authorName":"陆天虹","id":"50778bcf-b290-46fc-9541-e34adae3faf2","originalAuthorName":"陆天虹"},{"authorName":"王雪松","id":"f0a4954e-5528-4cfd-9277-646187309d6e","originalAuthorName":"王雪松"},{"authorName":"张宝文","id":"47cf39d0-69fd-43d9-88a6-3ecad5fe8da3","originalAuthorName":"张宝文"}],"doi":"10.3969/j.issn.1000-0518.2005.08.019","fpage":"895","id":"dae86ee4-2351-4af7-ac8d-743b9dcc1a85","issue":"8","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"5e2e4dd1-6d44-4bdd-914f-9506a249f23a","keyword":"马心<span style=\"color:red\">血红蛋白</span>","originalKeyword":"马心血红蛋白"},{"id":"2b94adde-a5bd-42f7-bc08-3f34e1a07bd2","keyword":"竹红菌甲素","originalKeyword":"竹红菌甲素"},{"id":"491964b5-2e1c-4a65-a8a4-b0da07862fd9","keyword":"相互作用","originalKeyword":"相互作用"},{"id":"22b21e6d-54e7-4324-b4ab-9d15074d7f5c","keyword":"UV-Vis吸收光谱","originalKeyword":"UV-Vis吸收光谱"},{"id":"bb041f8e-3ca8-469f-b689-f8503b5233e2","keyword":"荧光光谱","originalKeyword":"荧光光谱"},{"id":"5a517a31-1cf8-43e8-8fc4-9f590a63c77d","keyword":"荧光猝灭动力学常数","originalKeyword":"荧光猝灭动力学常数"}],"language":"zh","publisherId":"yyhx200508019","title":"竹红菌甲素与<span style=\"color:red\">血红蛋白</span>相互作用光谱","volume":"22","year":"2005"},{"abstractinfo":"将二氧化锆(Zro2)分散于壳聚糖(CS)中得到稳定的CS-ZrO2复合物,制备了Hb/nano-Au/CS-ZrO2/Au过氧化氢生物传感器.用循环伏安法和计时电流法考察该修饰电极的电化学特性,发现该修饰电极对过氧化氢(H2O2)的还原有良好的电催化作用.实验结果表明,该传感器对H2O2的线性响应范围为9×10-6～1.8×10-3mol/L,线性相关系数R=0.9956,检测下限为5.6×10-7mol/L(S/N=3 ),并具有选择性高、线性范围宽和响应快等优点.","authors":[{"authorName":"邓祥","id":"80205308-e6d2-40ac-a8e0-c18808674341","originalAuthorName":"邓祥"},{"authorName":"黄小梅","id":"2f8228d9-3542-47de-9233-0a8cf9b9d5b7","originalAuthorName":"黄小梅"}],"doi":"10.3724/SP.J.1095.2011.00210","fpage":"349","id":"7b3f5703-abbf-45dc-a0fb-27f43f4769c5","issue":"3","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"575f0900-5e09-4c14-a5d5-a0bb84ea6dbd","keyword":"二氧化锆","originalKeyword":"二氧化锆"},{"id":"34b56031-ffc6-41ce-b37d-8af64c803cbb","keyword":"壳聚糖","originalKeyword":"壳聚糖"},{"id":"cff437d8-4b56-4015-aa08-9e587f139607","keyword":"纳米金","originalKeyword":"纳米金"},{"id":"9fbcdc0e-716a-4e90-81e6-c276c2e1a7c7","keyword":"<span style=\"color:red\">血红蛋白</span>","originalKeyword":"血红蛋白"}],"language":"zh","publisherId":"yyhx201103019","title":"基于<span style=\"color:red\">血红蛋白</span>/纳米金/二氧化锆-壳聚糖修饰的过氧化氢生物传感器","volume":"28","year":"2011"},{"abstractinfo":"建立了采用变性高效液相色谱(DHPLC)对α-<span style=\"color:red\">血红蛋白</span>稳定<span style=\"color:red\">蛋白</span>(AHSP)基因进行基因分型和突变筛查的新方法.将AHSP基因序列分成6个片段,因第一、二、四、六个片段均含有1～2个常见单核苷酸多态性(single nucleotide polymorphism,SNP)位点,需单个标本分别进行检测;第三、五个片段不含有常见SNP位点,采用DHPLC结合DNA(脱氧核糖核酸)池的方法进行检测.以基因测序为金标准对所建立的AHSP基因检测方法进行方法学评价,结果显示:40个样品的DHPLC检测结果与测序结果之间完全吻合,说明所建立的检测方法能对AHSP基因6种常见SNP进行准确基因分型.应用DHPLC对365个样品的AHSP基因进行检测,发现2个罕见SNP(11810G＞A和12 802 C＞T);同时还发现2个错义突变(AHSP D29V和AHSP V56G),AHSP D29V突变为新突变,AHSP V56G为罕见突变.结果表明采用DHPLC法可有效地对AHSP基因进行基因分型和突变筛查.","authors":[{"authorName":"王志鹏","id":"39c223e8-ab2d-4e8e-9be1-e4bc24d4013c","originalAuthorName":"王志鹏"}],"doi":"10.3724/SP.J.1123.2011.00335","fpage":"335","id":"631a3433-135a-48ff-b8bc-d19d6f437fe9","issue":"4","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"7250200d-b027-4895-8187-e894001c6048","keyword":"变性高效液相色谱","originalKeyword":"变性高效液相色谱"},{"id":"b4df0474-8c30-4e80-b080-9164f438d0d8","keyword":"α-<span style=\"color:red\">血红蛋白</span>稳定<span style=\"color:red\">蛋白</span>基因","originalKeyword":"α-血红蛋白稳定蛋白基因"},{"id":"c3f98d0e-0bf2-4880-bcf1-8a0c604102b6","keyword":"基因分型","originalKeyword":"基因分型"},{"id":"c6dbd92e-450f-451b-8fbd-1a8ed5eb5df2","keyword":"突变筛查","originalKeyword":"突变筛查"}],"language":"zh","publisherId":"sp201104011","title":"变性高效液相色谱法在α-<span style=\"color:red\">血红蛋白</span>稳定<span style=\"color:red\">蛋白</span>基因检测中的应用","volume":"29","year":"2011"},{"abstractinfo":"合成和表征了L-半胱氨酸修饰的ZnS纳米粒子. 采用紫外光谱法、荧光光谱法、傅立叶衰减全反射红外光谱法(FTIR-ATR)研究了PBS缓冲体系中L-半胱氨酸修饰的ZnS纳米粒子与牛<span style=\"color:red\">血红蛋白</span>(Bovine Hemoglobin,BHb)的相互作用. 结果表明,L-半胱氨酸修饰的ZnS纳米粒子与BHb有较强的作用,BHb能使修饰后的ZnS纳米粒子发生荧光猝灭现象. 根据Stern-Volmer方程计算得到猝灭常数为1.12×106 L/mol. L-半胱氨酸修饰的ZnS纳米粒子很大程度上影响BHb的二级结构,使其构象发生变化.","authors":[{"authorName":"张雨琴","id":"495536af-14d6-4e04-a905-a9d975ca4923","originalAuthorName":"张雨琴"},{"authorName":"张友玉","id":"eb868578-dda4-4e5c-987a-605dad4ebfce","originalAuthorName":"张友玉"},{"authorName":"叶敏","id":"ae994785-8d80-4f39-983e-7a99e65b7c91","originalAuthorName":"叶敏"},{"authorName":"谭平","id":"a5828486-781e-4eee-9398-af9e37a7e991","originalAuthorName":"谭平"},{"authorName":"赵梦娇","id":"f687a880-9dbd-45c2-9cbf-bbbda8a43f8f","originalAuthorName":"赵梦娇"},{"authorName":"姚守拙","id":"06309ea4-3f58-4858-979d-ed7abd0d86a8","originalAuthorName":"姚守拙"}],"doi":"10.3969/j.issn.1000-0518.2008.09.003","fpage":"1011","id":"2b400435-70ac-43fb-893b-ca67d21a676a","issue":"9","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"d1374244-4771-4d51-9f1b-20fdcd40e1b7","keyword":"L-半胱氨酸","originalKeyword":"L-半胱氨酸"},{"id":"fa043b0d-1986-4128-8af9-a5717afb37a0","keyword":"ZnS纳米粒子","originalKeyword":"ZnS纳米粒子"},{"id":"078a6a32-4776-4575-93b6-db8491bbcd30","keyword":"牛<span style=\"color:red\">血红蛋白</span>","originalKeyword":"牛血红蛋白"},{"id":"ccc266da-2e28-42b4-a7e5-5cb337f79300","keyword":"紫外光谱","originalKeyword":"紫外光谱"},{"id":"48c4f95f-06a0-44f2-95e9-44548db416e3","keyword":"荧光光谱","originalKeyword":"荧光光谱"},{"id":"c701a1de-9dcf-45e3-a102-b30fe3818407","keyword":"FTIR-ATR","originalKeyword":"FTIR-ATR"}],"language":"zh","publisherId":"yyhx200809003","title":"光度法研究L-半胱氨酸修饰的ZnS纳米粒子与牛<span style=\"color:red\">血红蛋白</span>的作用","volume":"25","year":"2008"},{"abstractinfo":"在玻碳电极上修饰一层表面均匀的聚吡咯膜,然后利用钙钛矿和壳聚糖的复合膜来固定牛<span style=\"color:red\">血红蛋白</span>(Hb),制备出性能良好的过氧化氢生物传感器.采用循环伏安(CV)和扫描电镜对修饰电极进行了表征.该传感器对H2O2具有好的催化响应,且响应快.在优化的实验条件下,所制备的传感器对H2O2的线性范围为7.0×10-6～1.5×10-3mol/L,检测限为9.0×10-8mol/L.","authors":[{"authorName":"储艳文","id":"2f71f2b8-c918-43d7-a314-5df57f17e943","originalAuthorName":"储艳文"},{"authorName":"林生岭","id":"ae2e2b9e-c8fa-4146-9df7-0c63d13bdc32","originalAuthorName":"林生岭"},{"authorName":"郑佩","id":"8d6f978c-cf57-45d9-8212-728b07a40b48","originalAuthorName":"郑佩"},{"authorName":"郭寅丹","id":"81882fae-5a36-4ed4-b0a7-15d9962f0154","originalAuthorName":"郭寅丹"},{"authorName":"龙丹","id":"9a2229ed-6a17-4f77-aca6-3ac33931f68c","originalAuthorName":"龙丹"},{"authorName":"万勇","id":"e18a072e-ebbe-4568-bae4-afb45638fdb9","originalAuthorName":"万勇"}],"doi":"","fpage":"47","id":"e76da5c2-27a8-4dee-93c8-0cae95c84fb8","issue":"z1","journal":{"abbrevTitle":"GNCL","coverImgSrc":"journal/img/cover/GNCL.jpg","id":"33","issnPpub":"1001-9731","publisherId":"GNCL","title":"功能材料"},"keywords":[{"id":"787fa799-9281-4b1c-883e-b7a0de9c10b9","keyword":"聚吡咯","originalKeyword":"聚吡咯"},{"id":"105ce64c-ed80-4e26-8ae1-ea98a160a614","keyword":"La0.7Sr0.3NiO3","originalKeyword":"La0.7Sr0.3NiO3"},{"id":"9fdc8cfe-ce7c-4922-8b4e-3fc7dd494005","keyword":"壳聚糖","originalKeyword":"壳聚糖"},{"id":"3c61d2ff-9f7a-4a8f-8442-915e7f503c82","keyword":"牛<span style=\"color:red\">血红蛋白</span>","originalKeyword":"牛血红蛋白"},{"id":"18f6d64a-1e85-4305-b5b2-43952527a5a9","keyword":"过氧化氢传感器","originalKeyword":"过氧化氢传感器"}],"language":"zh","publisherId":"gncl2011z1013","title":"La0.7Sr0.36NiO3/壳聚糖/聚吡咯/牛<span style=\"color:red\">血红蛋白</span>修饰玻碳电极的制备及构建过氧化氢传感器","volume":"42","year":"2011"}],"totalpage":147,"totalrecord":1461}