Hou Jie
,
Gao Huichang
,
Wang Yingjun
,
Cheng Delin
,
Cao Xiaodong
材料科学技术（英文）
doi:10.1016/j.jmst.2016.08.013
Modifying substrates through mineralization is a popular way to improve the osteogenic performance. Screening of the best mineralization characteristics on specific substrates for stem cells is meaningful but not fully studied. In this paper, poly(lactic-co-glycolic acid)/hydroxyapatite (PLGA/HA, PH) microsphere scaffolds with superficial pores were fabricated by a low-temperature fusion method. After the mineralization in the 5× stimulated body fluid (SBF) for 0, 7, 12 and 24?h, four mineralized scaffolds (MPH-0, MPH-7, MPH-12 and MPH-24) with different apatite topographies were obtained. It was found that the surface of MPH-7 was evenly decorated with abundant micro-pores, MPH-12 with dense and plain apatite layer, and MPH-24 with small spherical bumps. The responses of mouse bone mesenchymal stem cells (mBMSCs) to the four scaffolds were further studied. The results showed that MPH-7 and MPH-24 had more obvious effects on mBMSCs attachment, proliferation and differentiation than MPH-0 and MPH-12. This work indicated that to obtain the maximum improvement, the mineralization characteristics had to be carefully chosen. This was noteworthy in the chemical modification of surfaces to form the functionalized scaffolds for bone repair.
关键词:
Microsphere scaffold
,
Mineralization
,
Topography
,
Cell behavior
Gao Huichang
,
Cao Xiaodong
,
Dong Hua
,
Fu Xiaoling
,
Wang Yingjun
材料科学技术（英文）
doi:10.1016/j.jmst.2016.01.011
In this paper, we fabricated three kinds of 3D microgrooves with different depth on biocompatible poly(lactic-co-glycolic acid) (PLGA) substrate via combination of soft-lithography and melt-casting methods, and investigated in detail their influence on C2C12 cell behaviors. It is found that cell proliferation, migration, alignment, spatial distribution, F-actin protein expression and gene expression are all remarkably distinct on these microgrooved samples and the smooth control PLGA substrate. The associated underlying mechanisms were further analyzed and discussed using real-time living cell monitoring, confocal laser scanning microscopy and gene microarray. Our preliminary results suggested that 3D microstructure could affect cell behaviors in a much more extensive manner than what we had understood before.
关键词:
Cell behaviors
,
3D microgroove
,
Poly(lactic-co-glycolic acid)
,
Cytoskeleton
,
Gene microarray
,
Real-time living cell monitoring
