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EFFECT OF MAGNETIC FIELD ON CALCITONIN GENE-RELATED PEPTIDE IN PERIODONTAL LIG AMENTS OF RATS

X. Chang , K. Qin and Y.L.Lu Orthodontic Department of Stomatology College , China Medical University , Sheny ang 110001 , China

金属学报(英文版)

proach change of CGRP with time in periodontal ligaments of rats in magnetic field, maxillae of 28 Wistar rats of 6 7 weeks were placed in magnetic fields of permanent magnets Nd 2 Fe 4 B. Changes of CGRP with t ime in maxillary periodontal ligaments of the rats were studied by immunohistochemistry. The result showed that: magnetic field had dynamic effect and some promotional effect on recove?E F F E C T O F M A G N E T I C F I E L D O N C A L C I T O N I N G E N E- R E L A T E D P E P T I D E I N P E R I O D O N T A L L I G A M E N T S O F R A T S X. Chang , K. Qin and Y. L. Lu Orthodontic Department of Stomatology College , China Medical University , Shenyang 110001 , China Manuscript received 24 June 1999 In order to approach change of C G R P with tim e in periodontal liga m ents of rats in m agneticfield , m axillae of 28 Wistar rats of 6 7 weeks w ere placed in m agnetic fields of per m anentm agnets Nd 2 Fe4 B. Changes of C G R P with tim e in m axillary periodontal liga ments of therats were stu died by i m m unohistoche mistry . The result sho wed that : m agnetic field had dy na m ic effect an d so m e pro motional effect on recovering and reconstruction of the periodontaltissues in it . This provided a better theoretical evidence for clinical use of per m anent m agnets .

关键词: per m anent m agnet Nd 2 Fe4 B , null , null , null

毛细管电泳法表征多肽及糖蛋白的稳定性

汪勇 , 高培峰 , 赵新颖 , 屈锋

色谱 doi:10.3724/SP.J.1123.2013.03052

建立了毛细管电泳表征多肽和糖蛋白稳定性的方法.分别以血管紧张素Ⅱ(Ang Ⅱ)和植物血球凝集素(PHA)、牛凝血酶(B-Thr)、人凝血酶(H-Thr)、辣根过氧化物酶(HRP)4种糖蛋白为多肽和糖蛋白的模式分子.从样品浓度、电泳缓冲液、样品溶液pH和离子强度等方面优化了血管紧张素Ⅱ的分离分析条件;从毛细管的选择、样品的电荷状态、电泳缓冲液的选择和分离电压的影响等方面讨论了糖蛋白的分离条件.Ang Ⅱ和4种糖蛋白的稳定性试验结果表明:Ang Ⅱ可在pH 7.4的硼酸盐缓冲液(0.02 mol/L)中于4℃下稳定放置48 h;4种糖蛋白可在pH 7.4硼酸盐缓冲液(0.2 mol/L)中于20,4,-20℃下稳定放置48 h;放置时间大于一周且小于四周时,在-20℃下各蛋白质均保持稳定;放置时间大于两周且小于四周时,只有HRP在上述3个温度下均保持稳定.该方法具有高效、快速、简单、低成本的特点,可广泛应用于多肽和蛋白质的稳定性表征.

关键词: 毛细管电泳 , 多肽 , 糖蛋白 , 稳定性

冷却速率对GWZ1032K合金中14H-LPSO结构形成的影响

张松袁广银卢晨丁文江

金属学报 doi:10.3724/SP.J.1037.2010.00177

采用金属型铸造和慢速凝固, 在不同冷却速率下制备了Mg-10Gd-3Y-1.8Zn-0.5Zr(质量分数, %) (GWZ1032K)合金. 采用SEM, TEM和XRD研究了冷却速率不同的GWZ1032K 合金的组织和相构成. 在GWZ1032K合金中, α-Mg基体中的层片状14H-LPSO结构随着冷却速率的下降而增加, 在冷却速率为0.005℃/s的试样中充满了整个晶粒;随着合金冷却速率降低, GWZ1032K合金中晶界第二相分别由5℃/s时的(Mg, Zn)3RE相转变为0.5 和0.1℃/s时的(Mg, Zn)3RE相和14H-LPSO结构的χ相共存;在0.01和0.005℃/s时只有14H-LPSO结构的$\chi$相. 结果显示在接近于平衡凝固的缓慢冷速条件下, 更容易形成具有稳定结构的层片状14H-LPSO结构和χ相.在冷却速率为0.5和0.1℃/s时, (Mg, Zn)3RE共晶相和χ相共存, (Mg, Zn)3RE共晶相和χ相的位向关系为 [110]χ phase//[223](Mg, Zn)3RE和∠g(001)χ phase g(110) (Mg, Zn)3RE=8.4°.

关键词: 14H-LPSO结构 , solidification rate , Mg-Gd-Y-Zn alloy , χ phase

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