{"currentpage":1,"firstResult":0,"maxresult":10,"pagecode":5,"pageindex":{"endPagecode":5,"startPagecode":1},"records":[{"abstractinfo":"本文对内涵高低压三级涡轮、涡轮出口支板通道、外<span style=\"color:red\">涵</span>通道以及内外<span style=\"color:red\">涵</span>混合段流动进行联立计算,给出了流场结构和流动分析.结果表明:联立数值模拟十分必要,是考察多部件匹配特性的有效手段.数值模拟的结果还表明:涡轮与支板的匹配不太理想,但气体通过支板后,仍能够接近轴向出气;混合段内外<span style=\"color:red\">涵</span>流动掺混作用并不强烈,由于掺混带来的气动损失并不严重.","authors":[{"authorName":"杨琳","id":"053d1cc1-4ea1-4e31-b50c-f6c3d55e9be9","originalAuthorName":"杨琳"},{"authorName":"刘火星","id":"c65c1356-937b-41b2-8c0d-411d724429d2","originalAuthorName":"刘火星"},{"authorName":"邹正平","id":"c9782795-0264-41b5-95c7-b7c73613c89b","originalAuthorName":"邹正平"},{"authorName":"李维","id":"ceddb533-21c1-49e8-adb7-eab4de3c1c4c","originalAuthorName":"李维"}],"doi":"","fpage":"39","id":"b28ac5ba-6854-49c4-b4e8-a17aa03a1c3a","issue":"1","journal":{"abbrevTitle":"GCRWLXB","coverImgSrc":"journal/img/cover/GCRWLXB.jpg","id":"32","issnPpub":"0253-231X","publisherId":"GCRWLXB","title":"工程热物理学报   "},"keywords":[{"id":"512b4656-e338-42e0-8905-ec566ab16599","keyword":"内外<span style=\"color:red\">涵</span>","originalKeyword":"内外涵"},{"id":"3697d4b9-2a43-4e2e-94ec-70ed7258db35","keyword":"涡轮","originalKeyword":"涡轮"},{"id":"e725fcbe-44fa-4f04-8dac-75268e6fa941","keyword":"支板","originalKeyword":"支板"},{"id":"bc177a4f-5313-4f07-8770-b79a9745901f","keyword":"数值模拟","originalKeyword":"数值模拟"},{"id":"50995557-d344-46b2-b768-4d324d5de3a5","keyword":"匹配","originalKeyword":"匹配"}],"language":"zh","publisherId":"gcrwlxb200601012","title":"涡轮内外<span style=\"color:red\">涵</span>联立数值模拟","volume":"27","year":"2006"},{"abstractinfo":"以咪唑和氯代乙酸乙酯为母体合成了N-咪唑基乙酸乙酯(IEA),用红外光谱表征了其结构,并用极化曲线法和交流阻抗法测试了其在NaHCO3及Na2SO4溶液中对金属铜的缓蚀性能. 实验证明,与咪唑相比,N-咪唑基乙酸乙酯分别在NaHCO3或Na2SO4质量分数为3%的溶液中(15 ℃)具有用量少,缓蚀性能好的特点. 在上述溶液中用量均为5 mg/L时,缓蚀效率分别可达86.8%和92.7%.","authors":[{"authorName":"<span style=\"color:red\">曾</span><span style=\"color:red\">涵</span>","id":"7374023c-d91b-44ca-a292-b9429b166e70","originalAuthorName":"曾涵"},{"authorName":"刘瑞泉","id":"c005a7d4-ee0e-4f38-b80c-fb9a69a4fe82","originalAuthorName":"刘瑞泉"},{"authorName":"王吉德","id":"efdb0493-589e-4ae8-8208-9267d44f0e67","originalAuthorName":"王吉德"},{"authorName":"张正方","id":"796789b1-aa97-476d-a952-aa2a52f761ff","originalAuthorName":"张正方"},{"authorName":"郭卫","id":"3ae029ca-9b31-436b-a04c-c83fee40085d","originalAuthorName":"郭卫"}],"doi":"10.3969/j.issn.1000-0518.2003.05.018","fpage":"487","id":"fa8352b6-2b01-4975-b0b5-4c90ef35ba61","issue":"5","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"47f6c31a-3638-4533-8734-9e7ca687af0b","keyword":"N-咪唑基乙酸乙酯,缓蚀剂,合成","originalKeyword":"N-咪唑基乙酸乙酯,缓蚀剂,合成"},{"id":"e90933fc-3553-46d9-aec4-f9ce99cb92e6","keyword":"铜","originalKeyword":"铜"}],"language":"zh","publisherId":"yyhx200305018","title":"N-咪唑基乙酸乙酯的合成及其缓蚀性能","volume":"20","year":"2003"},{"abstractinfo":"以2-溴乙酸、壳聚糖、2-硫代四氢咪唑啉酮为原料,合成了不同接枝率的壳聚糖-g-N-羧甲基-2-硫代-4,5-2H咪唑啉酮(CTS-g-CSIDZ),并对其理化性质进行了考察. 用配合滴定法测定了该聚合物对Cd~(2+)、CrO~(2-)_4、Fe~(3+)、Cu~(2+)、Pb~(2+)等重金属离子的吸附作用;进行了聚合物的悬菌定量实验,测定了接枝聚合物对一系列细菌的抑菌能力;采用失重法研究了合成的聚合物在1 mol/L HCl溶液中对N80钢片腐蚀的抑制作用. 结果表明,部分接枝的CSIDZ比小分子化合物具有更高的对测试的多数金属离子吸附作用的热稳定性;接枝CSIDZ具有较强的抑菌效果,最小抑菌浓度为5.10 g/L;接枝CSIDZ比小分子缓蚀剂具有更高的热稳定性,在90 ℃时对N80钢片的缓蚀效率仍然可以达到71.1 %,且用量仅为小分子化合物CSIDZ的1/2.","authors":[{"authorName":"<span style=\"color:red\">曾</span><span style=\"color:red\">涵</span>","id":"bd8d2cf6-a313-448d-ab8f-e844c37e512c","originalAuthorName":"曾涵"},{"authorName":"赵淑娴","id":"73c1fa2a-35b9-4038-b323-1575faa9f7ed","originalAuthorName":"赵淑娴"},{"authorName":"徐江玲","id":"a7669a68-91b2-45c3-aab4-2c2860970aa4","originalAuthorName":"徐江玲"},{"authorName":"郑孝伟","id":"31bdc3ab-f68e-45aa-a495-812a83b20ddf","originalAuthorName":"郑孝伟"}],"doi":"10.3969/j.issn.1000-0518.2009.11.008","fpage":"1287","id":"12b53393-2c82-49cd-94d2-5c8c55bf7932","issue":"11","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"73c5876b-99cb-4934-9f0b-a8d277b6c6d9","keyword":"N-羧甲基硫代-2H咪唑啉酮","originalKeyword":"N-羧甲基硫代-2H咪唑啉酮"},{"id":"55ae19a5-2b55-4f68-81af-3bd1ed7ade28","keyword":"壳聚糖","originalKeyword":"壳聚糖"},{"id":"ef470356-4a38-4b99-b7a8-89aad69ff297","keyword":"接枝聚合","originalKeyword":"接枝聚合"},{"id":"48681037-3cc9-4685-811a-3fcdc30cbe66","keyword":"抗菌能力","originalKeyword":"抗菌能力"},{"id":"01ef4722-8b84-43a7-a071-94c773223712","keyword":"金属离子吸附","originalKeyword":"金属离子吸附"},{"id":"5a6386e1-3212-492a-9983-b126ff37d324","keyword":"抑制腐蚀","originalKeyword":"抑制腐蚀"}],"language":"zh","publisherId":"yyhx200911008","title":"壳聚糖-g-N-羧甲基-2-硫代-4,5-2H咪唑啉酮的制备及其抑菌性能","volume":"26","year":"2009"},{"abstractinfo":"以氯乙酸乙酯、硫化钠、草酸二乙酯和壳聚糖为原料合成了3,4-二羟基噻吩交联壳聚糖(DTCC),测定了该交联聚合物对一系列菌种的抑菌能力;测定了以该交联聚合物为涂膜剂处理白桃后的失重率、腐烂率、叶绿素含量、膜透率和总糖含量随贮存时间的变化关系. 结果表明,部分交联的DTCC对革兰氏阳性、阴性菌有较强的抑菌效能,作用20 min后对铜绿假单胞菌、荧光假单胞菌、蜡样芽孢杆菌、枯草芽孢杆菌的抑菌率分别可达96.7%、95.8%、97.9%和98.2%,最小抑菌浓度分别为25.0、25.0、3.1和3.1 g/L;DTCC对黑曲霉菌和柑橘青霉菌亦有较强的抑菌效果,作用20 min后的抑菌率分别达到99.0%和28.4%,最小抑菌浓度分别为25.0和12.5 g/L. 以质量浓度为1.0 g/L的DTCC涂液处理白桃并在(27±0.5) ℃贮存12 d后,失重率仅有8.1%,腐烂率为40%,膜透率为34.1%,总糖含量可达75.1 mg/g.","authors":[{"authorName":"<span style=\"color:red\">曾</span><span style=\"color:red\">涵</span>","id":"ca8568d7-ec2c-4083-8798-ee9dd168e53a","originalAuthorName":"曾涵"},{"authorName":"赵淑贤","id":"2c5cd8c7-4f88-4737-a399-7356a12f846a","originalAuthorName":"赵淑贤"},{"authorName":"王永疆","id":"56b48d5d-8805-4228-a9c3-e07a1c90df2f","originalAuthorName":"王永疆"},{"authorName":"张雷","id":"2461a93c-e0f2-4eb0-a18f-6c7c08ba0e10","originalAuthorName":"张雷"}],"doi":"10.3724/SP.J.1095.2010.00037","fpage":"1265","id":"137f9c47-ccb1-4467-bb59-03a49c1cc94d","issue":"11","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"bb3bb101-2c3c-4794-b102-5275f40e596e","keyword":"壳聚糖","originalKeyword":"壳聚糖"},{"id":"c27d5d6f-2465-4d79-9b66-708fc2d9068e","keyword":"二羟基噻吩交联壳聚糖","originalKeyword":"二羟基噻吩交联壳聚糖"},{"id":"c5302068-fb3c-4c91-97b1-f9e6f46682d8","keyword":"保鲜","originalKeyword":"保鲜"},{"id":"4b0981fa-2b81-4840-9575-0c7abc7b0aa2","keyword":"抑菌性能","originalKeyword":"抑菌性能"}],"language":"zh","publisherId":"yyhx201011005","title":"3,4-二羟基噻吩交联壳聚糖的合成及其对白桃的抑菌保鲜性能","volume":"27","year":"2010"},{"abstractinfo":"以纳米金溶胶(NGS)和多重壁碳纳米管(MWCNTs)的共混物(NGS/MWCNTs)作为固定漆酶的载体,研究了3种固定漆酶方法在酶固定量、比活力上的差异. 研究了不同的固定方法对固定酶热稳定性和重复使用性及纳米金溶胶颗粒粒径对酶固定量和固定酶动力学参数的影响. 实验结果表明,NGS/MWCNTs具有良好的固定漆酶能力和高固酶比活力,NGS/MWCNTs(NGS粒径37 nm)通过简单物理吸附法固定漆酶的量和固酶的比活力最高,分别可达33.80 mg/g和9.433 U/mg. 在NGS/MWCNTs上采用化学键合方法固定的漆酶在70 ℃放置2 h后仍然保持初始活力的75%,重复使用20次后仍保持初始活力的70%. 纳米金溶胶粒子越小(24 nm),底物和固定漆酶间亲和力越好(KM=0.027 mmol/L),表观速率常数越大.","authors":[{"authorName":"<span style=\"color:red\">曾</span><span style=\"color:red\">涵</span>","id":"928878dd-19dc-4a1c-a35d-4de0ba98e93a","originalAuthorName":"曾涵"},{"authorName":"尹筱莉","id":"92df407a-2f5e-4850-b03d-44062d33ce70","originalAuthorName":"尹筱莉"},{"authorName":"杨忠丽","id":"91195d53-6174-4a78-9ccf-59064fa5305b","originalAuthorName":"杨忠丽"},{"authorName":"徐江玲","id":"062e9c48-7657-423c-b6be-04dc47ae85c7","originalAuthorName":"徐江玲"},{"authorName":"张永全","id":"bd270822-9c5d-4d99-a8b3-c9dcf8235fa2","originalAuthorName":"张永全"}],"doi":"10.3724/SP.J.1095.2010.90433","fpage":"829","id":"ced56d47-cc86-4f97-9541-f8b9830bcbda","issue":"7","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"a32e4a06-78fb-4cb0-b89a-d952a3a07493","keyword":"漆酶","originalKeyword":"漆酶"},{"id":"f8efed4e-25c8-4b4f-85b5-8c113dda0f6f","keyword":"酶固定","originalKeyword":"酶固定"},{"id":"771a94d7-a623-4470-8797-d5f965a913c1","keyword":"纳米金溶胶","originalKeyword":"纳米金溶胶"},{"id":"5ce96cb2-b5d9-4948-aed3-8b3b504ba26d","keyword":"粒子尺寸效应","originalKeyword":"粒子尺寸效应"},{"id":"e5181df3-2ee5-4de0-8712-1551dd4cf0a2","keyword":"热稳定性","originalKeyword":"热稳定性"},{"id":"4058566a-316d-4e3f-9374-74c0ec8696d5","keyword":"重复使用性","originalKeyword":"重复使用性"}],"language":"zh","publisherId":"yyhx201007017","title":"漆酶在纳米金溶胶/多重壁碳纳米管复合载体上固定方法的比较及粒子尺寸效应","volume":"27","year":"2010"},{"abstractinfo":"以N,N′-亚甲基双丙烯酰胺(BIS)交联聚甲基丙烯酸作为固定漆酶的载体,以共价偶联法固定云芝漆酶并测定了固定基元的酶固定量和固定漆酶的比活力. 还研究了固定漆酶热稳定性、重复使用性以及固定漆酶催化2,6-二甲氧基苯酚(DMP)氧化的酶动力学参数. 实验结果表明,这种交联聚合物基元通过共价偶联法固定漆酶的量和固定漆酶的比活力分别可达26.37 mg/g和1.202 U/mg;在交联聚合物基元上固定的漆酶在50 ℃下放置2 h后仍然保持初始活力的83%,重复使用10次后仍保持初始活力的80%以上;交联聚合物固定漆酶催化DMP氧化的表观速率常数kcat可达1090 min-1,以固定漆酶的BIS交联聚甲基丙烯酸功能化碳纳米管修饰的玻碳电极在pH=4.4磷酸盐缓冲液中氧还原发生在+724 mV(vs.SCE).","authors":[{"authorName":"<span style=\"color:red\">曾</span><span style=\"color:red\">涵</span>","id":"eb18e78c-e108-41be-b1f9-97dbf52c8a07","originalAuthorName":"曾涵"},{"authorName":"赵淑贤","id":"e0da4357-32e6-4a9c-b8fe-3bd3bb2651f5","originalAuthorName":"赵淑贤"},{"authorName":"龚兰新","id":"47a7ad11-4f3a-41df-9816-92d6cc7dde47","originalAuthorName":"龚兰新"},{"authorName":"许国强","id":"6817fb0d-16fb-409a-9432-7b80d5e07937","originalAuthorName":"许国强"}],"doi":"10.3724/SP.J.1095.2010.90742","fpage":"1076","id":"14e84e6e-cea0-42c6-8ecd-7ecb5c0ed12a","issue":"9","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"21406a76-3da5-4d7b-8264-dc81771e44d2","keyword":"漆酶","originalKeyword":"漆酶"},{"id":"a9a23b61-6c55-4f64-8fd5-c5b2545b802f","keyword":"酶固定","originalKeyword":"酶固定"},{"id":"9de610f3-df9b-4b62-931d-e11e83b4bb82","keyword":"热稳定性","originalKeyword":"热稳定性"},{"id":"0366f8b2-aef4-4513-84c0-f6535ad3cfe2","keyword":"酶动力学","originalKeyword":"酶动力学"},{"id":"f49b7f32-e1c7-4272-b965-a402617908c8","keyword":"氧还原","originalKeyword":"氧还原"}],"language":"zh","publisherId":"yyhx201009018","title":"云芝漆酶在N,N′-亚甲基双丙烯酰胺(BIS)交联聚甲基丙烯酸基元上的固定及其修饰玻碳电极电化学行为","volume":"27","year":"2010"},{"abstractinfo":"以四氯金酸和L-半胱氨酸为原料,合成了L-半胱氨酸功能化纳米金粒子,将此纳米粒子修饰在金盘电极表面并共价偶联漆酶分子.以循环伏安法研究了此固定漆酶电极在无氧磷酸盐缓冲液中电化学行为和催化氧还原能力,并进一步评估了其作为氧传感器使用的性能:以计时电流法测定其对氧气的检测限,与氧气的亲和力(以米氏常数表征),研究了传感器的长期使用性、热稳定性和pH-催化电流关系.结果表明,此固定漆酶电极可以实现漆酶活性中心T2与导电纳米粒子间的直接电子迁移而无需任何外加电子中介体(氧化还原峰的式电位为192.5 mVvsAgCl/Ag),并在接近漆酶活性中心T3氧化还原式电位(780 mV vs NHE)附近发生氧还原；测得的固酶电极与氧气的亲和力较高(米氏常数为216.4 μmol/L)且检测限低达0.22 μmol/L,在4℃下保存60d后活力仍然保持初始活力的大约78％.但这种电极的热稳定性较差,受pH值影响较明显,在pH值近于生理条件时几乎完全丧失活力.","authors":[{"authorName":"<span style=\"color:red\">曾</span><span style=\"color:red\">涵</span>","id":"06a60ae2-e675-4286-9062-d8ab427448fc","originalAuthorName":"曾涵"},{"authorName":"崔婷","id":"19b6661e-2ce0-4246-bca0-3e03233bb748","originalAuthorName":"崔婷"},{"authorName":"常伟","id":"bca072fc-3e04-4f18-b24b-44aa660bb800","originalAuthorName":"常伟"},{"authorName":"侯凤兰","id":"f50e9baf-5f63-4016-a77f-2d2ce0814efb","originalAuthorName":"侯凤兰"}],"doi":"10.3724/SP.J.1095.2011.00020","fpage":"1421","id":"2a30cbf4-ec25-4c5e-9881-bb4d1547fcdd","issue":"12","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"e0bac799-b675-41b9-984d-78b0f8aa173c","keyword":"漆酶","originalKeyword":"漆酶"},{"id":"7a131977-6be4-4c3a-8576-6167db54bfc2","keyword":"纳米金粒子","originalKeyword":"纳米金粒子"},{"id":"6196153b-367b-457f-bdb7-fb54e06251cd","keyword":"氧传感器","originalKeyword":"氧传感器"},{"id":"fd9ca7a4-9c99-43f1-92bd-9f315a6c78cd","keyword":"直接电子转移","originalKeyword":"直接电子转移"}],"language":"zh","publisherId":"yyhx201112014","title":"L-半胱氨酸/漆酶修饰的纳米金阴极对氧还原的电催化","volume":"28","year":"2011"},{"abstractinfo":"制备了壳聚糖-g-N-羧甲基-二(2-苯并咪唑)-1,2-乙二醇(CTS-g-N-CBBIE),将其与纯化的纳米金溶胶(NGS)共混得到CTS-g-N-CBBIE-NGS复合物.以此复合物作为固酶载体固定云芝漆酶,固酶量大(31.10 mg/g),固酶比活力高(1.43 U/mg);此固酶复合物修饰的玻碳电极在无氧磷酸盐-柠檬酸盐缓冲溶液(pH=5.0)中可以实现无中介酶-电极直接电子迁移(一对准可逆氧化还原峰式电位576 mv(vs.Ag/AgCl)对应于漆酶活性中心T1位的氧化还原),电子迁移速率常数为228.3 s-1.当氧气浓度较小时,这种固酶修饰电极对氧气还原具有一定的生物电催化性能(空气饱和缓冲溶液中氧还原峰电位约为320mv(vs.Ag/AgCl)).当氧气浓度增高后,氧还原反应受到抑制;但这种漆酶修饰电极对pH较为敏感,且稳定性和重复使用性欠佳.","authors":[{"authorName":"<span style=\"color:red\">曾</span><span style=\"color:red\">涵</span>","id":"a5936005-a3c7-4f2e-8563-f7feb8ae79ad","originalAuthorName":"曾涵"},{"authorName":"赵淑贤","id":"caf3beda-a52a-4dfc-9a8b-a7707b6ff9cb","originalAuthorName":"赵淑贤"},{"authorName":"龚兰新","id":"85d23663-7ca3-4180-9d91-ddcd3aa74dac","originalAuthorName":"龚兰新"},{"authorName":"张国军","id":"66fc4cc7-9e77-4d15-bf22-cfd94b130bf4","originalAuthorName":"张国军"}],"doi":"10.3724/SP.J.1095.2011.00304","fpage":"326","id":"96cf0975-12d8-4821-b769-7928fee6b510","issue":"3","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"f1e441a6-157d-43e4-89ac-44e672c58533","keyword":"壳聚糖-g-N羧甲基-二(苯并咪唑)乙二醇","originalKeyword":"壳聚糖-g-N羧甲基-二(苯并咪唑)乙二醇"},{"id":"e81ff09d-8d11-4447-ba12-688f1104568a","keyword":"直接电子迁移","originalKeyword":"直接电子迁移"},{"id":"5da0b1c2-61da-4a63-ae50-552421bf26c6","keyword":"纳米金溶胶","originalKeyword":"纳米金溶胶"},{"id":"d184e3cd-fb75-4ea7-a168-8732f377a84e","keyword":"漆酶","originalKeyword":"漆酶"},{"id":"33d21659-67a3-4012-b63c-1fdedd4af07a","keyword":"生物电催化","originalKeyword":"生物电催化"}],"language":"zh","publisherId":"yyhx201103015","title":"壳聚糖-g-N-羧甲基-二(2-苯并咪唑)-1,2-乙二醇和纳米金溶胶复合物固定漆酶修饰玻碳电极的直接电化学","volume":"28","year":"2011"},{"abstractinfo":"通过壳聚糖-g-N-羧甲基-2-硫代-4,5-2H咪唑啉酮(CTS-g-N-CSIDZ)非共价功能化多壁碳纳米管(MWCNTs)的方式制备固定漆酶载体,该复合物载体主要通过物理吸附和漆酶活性中心与载体上配体之间的配位作用来固定漆酶,较大程度地保持了游离漆酶活性位原始构象.将固定了漆酶的复合物附着在裸玻碳电极上便构筑了复合物固定漆酶修饰玻碳电极.在以分光光度法测定了这种复合物载体对漆酶的担载量、固定漆酶比活力、稳定性、重复使用性及其催化2,6-二甲氧基苯酚(DMP)氧化动力学参数的基础上,还对基于此种复合物固定漆酶修饰玻碳电极作为化学传感器(以DMP作为底物)的性能进行了研究.结果表明,该复合物具有较高的固酶担载量(81.7 mg/g)和固定漆酶比活力(1.33 U/mg)；而作为电化学传感器的复合物固定漆酶修饰玻碳电极对底物DMP具有较高的亲和力(对DMP的米氏常数KM是0.0918 mmol/L),较高的灵敏度( 3680 mA· L/mol),较低的检测限(3.3×10-4 mmol/L),较高的响应选择性,良好的重现性、重复使用性和长期稳定性.这种漆酶基电极有望用作电流型特定结构的酚类传感器.","authors":[{"authorName":"<span style=\"color:red\">曾</span><span style=\"color:red\">涵</span>","id":"dca27ad7-8a93-47f5-bfe6-8a60aa54f88e","originalAuthorName":"曾涵"},{"authorName":"龚兰新","id":"3d39680c-b9ed-494c-8d9b-5b982160bd14","originalAuthorName":"龚兰新"}],"doi":"10.3724/SP.J.1095.2012.00260","fpage":"462","id":"24ae32e7-f5b7-46f3-98dd-619e03c499f5","issue":"4","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"f75a8f50-a5d3-4eb5-9759-d71c4e6cb511","keyword":"漆酶","originalKeyword":"漆酶"},{"id":"fd0b349b-140d-41cc-b891-e76195c8b52f","keyword":"多壁碳纳米管","originalKeyword":"多壁碳纳米管"},{"id":"c1afa7de-c1f2-49a1-a1c4-45927068fdff","keyword":"壳聚糖","originalKeyword":"壳聚糖"},{"id":"2cb7a0a3-20ed-452c-a012-673c789d5fb9","keyword":"酶动力学","originalKeyword":"酶动力学"},{"id":"ef549597-d8e3-4669-9263-f0d1d8b02185","keyword":"化学传感器","originalKeyword":"化学传感器"}],"language":"zh","publisherId":"yyhx201204016","title":"壳聚糖-g-N-羧甲基-2-硫代-4,5-2H咪唑啉酮-多壁碳纳米管复合物固定漆酶基化学传感器的性能","volume":"29","year":"2012"},{"abstractinfo":"采用不同结构的高分子聚合物与纯化的多壁碳纳米管(MWCNTs)共混的方法,制备得到聚合物非共价功能化多壁碳管复合物,测定了这些载体对漆酶(lac)的担载量、固定漆酶的比活力及稳定性.以固定漆酶的复合物修饰玻碳(GC)电极后,采用循环伏安法研究这些电极在无氧磷酸盐缓冲液(PBS)中的直接电化学行为及催化氧还原活力,粗略地测定了固定漆酶与电极间电子转移的速率常数.实验结果表明,当聚合物中含亲漆酶基团或能与漆酶活性中心发生相互作用的官能团时利于直接电子转移,而且复合物固定漆酶保持了游离漆酶的天然构象.这些电极中,lac/NIPAM-co-BPCP-M WCNTs/GC(NIPAM-co-BPCP:N-烯丙基-1-苯甲酰基-3-苯基-4,5-2H-4-甲酰胺基吡唑-co-N-异丙基丙烯酰胺)在无氧PBS中发生直接电子转移的式电位(605mV)更接近漆酶活性中心的式电位(580mV),具有较快的异相电子转移速率(0.726s-1),较高的漆酶担载量(103.5mg/g)和固定漆酶比活力(1.68U/mg),较高的催化氧还原能力(氧还原起始电位820mV,在650mV时的催化峰电流为85.5μA)以及良好的重复使用性和长期使用性.","authors":[{"authorName":"<span style=\"color:red\">曾</span><span style=\"color:red\">涵</span>","id":"44320877-1c93-4fd1-9585-f4d1f2d16c8a","originalAuthorName":"曾涵"},{"authorName":"龚兰新","id":"e3fea5d4-8f5f-4042-918e-dd8e2f425cc4","originalAuthorName":"龚兰新"}],"doi":"10.3724/SP.J.1095.2012.00288","fpage":"682","id":"8ba39ed1-934c-460f-828c-2679c824119b","issue":"6","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"91af00d6-5b64-45f7-a74b-9b42a1d07929","keyword":"漆酶","originalKeyword":"漆酶"},{"id":"00828bb5-ee4d-4f91-b827-9abb3a00bb65","keyword":"多重壁碳纳米管","originalKeyword":"多重壁碳纳米管"},{"id":"7badb83c-af2a-4d56-a34b-90a8eb4d1c69","keyword":"高分子聚合物","originalKeyword":"高分子聚合物"},{"id":"6b2464d8-c33c-4aee-861b-59e0a2f73de5","keyword":"玻碳电极","originalKeyword":"玻碳电极"},{"id":"f231ad4c-8d3b-45b7-9d64-a67f70e631bc","keyword":"直接电子转移","originalKeyword":"直接电子转移"}],"language":"zh","publisherId":"yyhx201206013","title":"高分子聚合物-多壁碳纳米管复合物固定漆酶及其在玻碳电极上的直接电子转移","volume":"29","year":"2012"}],"totalpage":15,"totalrecord":145}