以海藻酸盐、聚精氨酸为壁材,采用高压静电法制备了球形度好、表面光洁、粒径均匀的新型药物载体--海藻酸钙/聚精氨酸微胶囊.参照医疗器械生物学评价标准,对其生物相容性进行了研究.细胞毒性试验结果显示,当海藻酸钙/聚精氦酸微胶囊的含量为0.1、0.5、1.0mg/mL时,微胶囊对L929细胞生长无明显抑制作用,海藻酸钙/聚精氦酸微胶囊浸提产物在10.0 mg/mL时仍无细胞毒性作用.海藻酸钙/聚精氨酸微胶囊不引起急性全身毒性反应,不引起溶血反应.通过本组试验可见,采用高压静电法制备的药物载体--海藻酸钙/聚精氨酸微胶囊具有较好的生物相容性,具有开发和应用价值.
参考文献
| [1] | Wang S B,Xu F H,He H S,et al.Novel alginate-poly(Lhistidine)microcapsules as drug carriers:In vitro protein release and short term stability[J].Macromolecular Bioscience,2005,5(5):408-414. |
| [2] | Mohamed F,van der Walle C F.PLGA microcapsules with novel dimpled surfaces for pulmonary delivery of DNA[J].International Journal of Pharmaceutics,2006,311(1/2):97-107. |
| [3] | Ding H F,Liu R,Li B G,et al.Biologic effect and immunoisolating behavior of BMP-2 gene-transfected bone marrow-derived mesenchymal stem cells in APA microcapsules[J].Biochemical and Biophysical Research Communications,2007,362(4):923-927. |
| [4] | Wang X Q,Wenk E,Hu X,et al.Silk coatings on PI,GA and alginate microspheres for protein delivery[J].Biomaterials,2007,28(28):4161-4169. |
| [5] | 沈一丁,赖小娟,王磊,聚乳酸/乙基纤维素复合膜的制备及其性能[J].复合材料学报,2007,24(3):40-44.Shen Yiding,Lai Xiaojuan,Wang Lei.Preparation and properties of poly(lactic acid)/ethy1 cellulose composite films[J].Acta Materiae Compositae Sinica,2007,2443):40-44. |
| [6] | Mitchell D J,Kim D T,Steinman L,et al.Polyarginine enters cells more efficiently than other polycationic homopolymers[J].Journal of Peptide Research,2000,56(5):318-325. |
| [7] | Ohtake K,Maeno T,Ueda H,et al.Poly-L-arginine predominantly increases the paracellular permeability of hydrophilic macromolecules across rabbit nasal epithelium in vitro[J].Pharmaceutical Research,2003,2042):153-160. |
| [8] | 刘怡晟,鲍倩玲,张伟利,精氨酸强化全肠外营养对小肠上皮细胞增殖与凋亡的影响[J].肠外与肠内营养,2007,14(1):6-9.Liu Yisheng,Bao Qianling,Zhang Weili.Effects of arginineenriched TPN on proliferation and apoptosis of intestinal epithelial cells[J].Parenteral & Enteral Nutrition,2007,14(1):6-9. |
| [9] | Lingnau K,Egyed A,Schellaek C,et al.Poly-L-arginine synergizes with oligodeoxynucleotides containing CpG-motifs (CpG-ODN)for enhanced and prolonged immune responses and prevents the CpG-ODN-induced systemic release of proinflammatory cytokines[J].Vaccine,2002,20(29/30);3498-3508. |
| [10] | Shi H P,Wang S M,Zhang G X,et al.Supplemental L-arginine enhances wound healing following trauma/hemorrhagic shock[J].Wound Repair and Regeneration,2007,15(1):66-70. |
| [11] | Neto H S,Vomero V U,Marques M J.L-arginine enhances muscle regeneration after experimental envenomation by B.jararacussu:A future for nitric oxide-based therapy?[J].Toxicon,2006,48(3):353-357. |
| [12] | Wang S B,Liu YG,Weng L J,et al.Effect of the molecular weights of poly-L-arginine on membrane strength and permeability of poly-L-arginine group microcapsules[J].Macromolecular Bioscience,2003,3(7):347-350. |
| [13] | Wang S B,Chen A Z,Weng L J,et al.Effect of drug-loading methods on drug load,encapsulation efficiency and release properties of alginate/poly-L-arginine/chitosan ternary complex microcapsules[J].Macromolecular Bioscience,2004,4(1):21-30. |
| [14] | 王莹,刘伟,王士斌,等,海藻酸钙微胶珠的载药性能优化[J].生物医学工程研究,2007,26(3):271-274.Wang Ying,Liu Wei,Wang Shibin,et al.Optimize conditions of preparation calcium alginate micro beads[J].Journal of Biomedical Engineering Research,2007,26(3):271-274. |
| [15] | Cheng N,Liu W G,Cao Z Q,et al.A study of thermoresponsive poly 4 N-isopropylacrylamide)/polyarginine bioconjugate non-viral transgene vectors[J].Biomaterials,2006,27(28):4984-4992. |
| [16] | Chellag F,Tabrizian M,Dumitriu S,et al.In vitro and in vivo biocompatibility of chitosan-xanthan polyionic complex[J].J Biomed Mater Res,2000,51(1):107-116. |
| [17] | de groot C J,van Luyn M J A,van Dijk-Wolthuis W N E,et al.In vitro biocompatibility of biodegradable dextran-based hydrogels tested with human fibroblasts[J].Biomaterials,2001,22(11):1197-1203. |
| [18] | Sukhotnik I,Helou H,Mogilner J,et al.Oral arginine improves intestinal recovery following ischemia-reperfusion injury in rat[J].Pediatric Surgery International,2005,21(3):191-196. |
| [19] | 国家技术监督局,GB/T 16175-1996医用有机硅材料生物学评价试验方法[S].北京:中国标准出版社,2003. |
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