设计合成了3种模拟胶原三螺旋结构或/和整合素识别位点的胶原模拟多肽（CMP）,对其进行体外细胞相容性评价,研究其对小鼠成纤维细胞（L929）生长、粘附的影响。实验证实,3种CMP对成纤维细胞生长无明显的细胞毒性反应;3种包被胶原模拟多肽的基底均能在一定程度上促进细胞粘附、生长,具有良好的细胞粘附率和细胞附着形态,其中包含三螺旋结构和整合素识别位点的CMP27具有更好的促粘附效果,细胞粘附数量和形态与胶原接近。初步研究结果证实,胶原三螺旋结构与整合素识别位点共同作用促进L929细胞粘附。因此,CMP可以有效促进细胞粘附,有望作为粘附剂应用于生物医学领域,可为设计以多肽为基础的生物活性材料提供新的研究思路。
Collagen mimetic peptides （CMPs） incorporating the triple-helical sequence or/and integrin-binding sequence were designed. To evaluate biocompatibility of CMPs in vitro, fibroblast cells （L929） were cultured. The proliferation and attachment of cell were observed in certain time. It is demonstrated that all CMPs had no obvious effect on the proliferation of L929 cells. The results also show that all CMPs promote migration and at- tachment of L929 to the coated surface. And the cells attachment shape and proliferation rate of L929 were good. After coating with CMP27 which containing the triple-helical sequence and integrin-binding sequence, the cell adhesion and spreading of L929 were significantly improved compared with others, and comparable to that observed on type I collagen. The triple-helical sequence and the integrin-binding sequence were shown to be im- portant roles on promoting cell adhesion collaboratively. Therefore, CMP is effective for improving the bioactiv- ity of cell adhesion, and could be potentially used as an adhesive for biomedical application. Moreover, this study provided new insights in designing other peptide-based bioaetivity materials.
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