通过离子凝胶法制备出球形完好的海藻酸钠-胰蛋白酶微球.红外分析表明,微球中凝胶基质通过海藻酸钠的-COO-与Ca2+发生静电作用交联形成.SEM观察显示微球中存在蛋格结构及孔洞.考察了不同因素对微球载药率、包封率和体外释药率的影响.结果表明,海藻酸钠水溶液浓度越高释药率越低,当海藻酸钠与胰蛋白酶质量比为4,海藻酸钠水溶液浓度为4%时呈最大的包封率和载药量;随着海藻酸钠与胰蛋白酶质量比增大,载药率降低,包封率呈先增后降变化趋势.
参考文献
| [1] | Wu Kai,Xu Zhenliang,Wei Yongming.Sodium alginatrpolyvinyl alcohol/polysulfone(SA-PVA/PSF)hollow fiber composite pervaporation membrane for dehydration of ethanolwater solution[J].J Shanghai Univ:Engl Ed,2008,12(2):163-170. |
| [2] | Li Sha,Wang Xiangtao,Zhang Xiaobin.Studies on alginatechitosan microcapsules and renal arterial embolization in rabbits[J].Journal of Controlled Release,2002,84:87-98. |
| [3] | Hurteaux R,Edwards-L'evy F,Laurent-Maquin D,MarieChristine L.Coating alginate mierospheres with a serum albumin-alginate membrane:Application to the encapsulation of a peptide[J].European Journal of PharmaceuticalSciences,2005,24:187-197. |
| [4] | Jang-Wook L,Cheau-Yih T,Sang-Kyung L,Yong-Hee K,L Kuen-Yong.Controlled delivery of heat shock protein using an injectable microsphere/hydrogel combination system for the treatment of myocardial infarction[J].Journal of Controlled Release,2009,137:196-202. |
| [5] | Wells L A,Sheardown H.Extended release of high pl proteins from alginate microspheres via a novel encapsulation technique[J].European Journal of Pharmaceutics and Biopharmaceutics,2007,65:329-335. |
| [6] | Coppi G,Iannuccelli V,Bernabei M T,Cameroni R.Alginate microparticles for enzyme peroral administration[J].International Journal of Pharmaceutics,2002,242:263-266. |
| [7] | Hori Y,Winans A M,Huang C C,Horrigan E M,Irvine D J.Injectable dendritic cell-carrying alginate gels for immunization and immunotherapy[J].Biomaterials,2008,29:3671-3682. |
| [8] | Bowersocka T L,HogenEsch H,Suckow M,Guimond P,Martin S.Oral vaccination of animals with antigens encapsulated in alginate microspheres[J].Vaccine,1999,17:1804-1811. |
| [9] | Bowersock T L,HogenEsch H,Suckow M,Porter R E,Jackson R.Oral vaccination with alginate microsphere systems[J].Journal of Controlled Release,1996,39:209-220. |
| [10] | Lemoine D,Wauters F,Bouchend homme S,Pre'at V.Preparation and characterization of alginate microspheres containing a model antigen[J].International Journal of Pharmaceutics,1998,176:9-19. |
| [11] | 邓芹英,刘岚,邓慧敏.波普分析教程[M].第2版.北京:科学出版社,2007:55. |
| [12] | Dong Zhanfeng,Wang Qun,Du Yumin.Alginate/gelatin blend films and their properties for drug controlled release[J].Journal of Membrane Science,2006,280:37-44. |
| [13] | Ant'onio J R,Catarina S,Domingos F.Chitosan-reinforced alginate microspheres obtained through the emulsification/internal gelation technique[J].European Journal of Pharmaceutical Sciences,2005,25:31-40. |
| [14] | Fundueanu G,Esposito E,Mihai D.Preparation and characterization of Ca-alginate microspheres by a new emulsification method[J].International Journal of Pharmaeeutics,1998,170:11-21. |
| [15] | Chan L W,Jin Y,Heng P W S.Cross-linking mechanisms of calcium and zinc in production of alginate microspheres[J].International Journal of Pharmaceutics,2002,242:255-258. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%