以聚己内酯二醇为软段、赖氨酸二异氰酸乙酯为硬段和异山梨醇为扩链剂,用二步逐步聚合法合成了医用可降解的聚氨酯;采用相转变-粒子沥滤法制备聚氨酯多孔三维支架,并考察了支架的形貌,孔径大小、分布和支架的力学性能,用小鼠畸胎瘤细胞进行了支架的细胞增殖实验.结果表明,制备出的聚氨酯支架由大小不同的孔构成,孔隙率超过75％;其中添加不良溶剂甲醇和水制备得到的支架,孔与孔之间连通性较好,具有良好的抗压性能.细胞增殖测试结果表明,这种聚氨酯支架支持细胞的生长和增殖.
A two-step method was used to synthesize a novel biodegradable polyurethane (PU) composed by L-lysine ethyl ester diisocyanate (LDI),poly (ε-caprolactone) diols (PCL-diol) and 1,4∶ 3,6-dianhydro-D-sorbitol (Isosorbide) as chain extender.3D porous PU scaffolds were produced by a combined salt leaching-phase inverse technique.The surface structure,pore size,pore size distribution,pore interconnectivity and mechanical properties of the scaffolds were investigated.The bio-safety of PU was evaluated with cell proliferation of mouse ATDC5.Results show that micorpores and macropores distributed in the produced scaffolds with high porosity (more than 75％).The scaffolds that were prepared with addition of methanol and water have good tensile strength,with well interconnected pores.In addition,the CCK-8 expriment result displayed that the cells could survive and proliferate on PU scaffolds.
参考文献
| [1] | Ruecker,M.,Laschke,M.W.,Junker,D.,Carvalho,C.,Schramm,A.,Muelhaupt,R.,Gellrich,N.-C.,Menger,M.D.,Angiogenic and inflammatory response to biodegradable scaffolds in dorsal skinfold chambers of mice,Biomaterials,27,5027(2006) |
| [2] | Hak-Joon,S.,Meredith,C.,Johnson,C.,Galis,Z.S.,The effect of scaffold degradation rate on three-dimensional cell growth and angiogenesis,Biomaterials,25,5735(2004) |
| [3] | Goma,K.,and Gogolewski,S.,Biodegradable porous polyurethane scaffolds for tissue repair and regeneration,Journal of Biomedical Materials Research Part A,79A,128(2006) |
| [4] | LI Baoqiang,HU Qiqolin,FANG Zhengping,XU Chengwei,Progress in polyurethane for tissue engineering,Polymer Bulletin,2,1 (2003)(李保强,胡巧琳,方征平,许承威,组织工程用聚氨酯的研究进展,高分子通报,2,1(2003)) |
| [5] | Zhang,J.Y.,Beckman,E.J.,Piesco,N.P.,Agarwal,S.,A new peptide-based urethane polymer:synthesis,biodegradation,and potential to support cell growth in vitro,Biomaterials,21,1247 (2000) |
| [6] | Goma,K.,Polowinski,S.,and Gogolewski,S.,Synthesis and characterization of biodegradable poly(ε-caprolactone urethane)s.I.Effect of the polyol molecular weight,catalyst,and chain extender on the molecular and physical characteristics,Journal of Polymer Science PartA:Polymer Chemistry,40,156(2002) |
| [7] | He,X.,Zhai,Z.,Wang,Y.,Wu,G.,Zheng,Z.,Wang,Q.,Liu,Y.,New method for coupling collagen on biodegradable polyurethane for biomedical application,Journal of Applied Polymer Science,126,E354(2012) |
| [8] | HE Xianyun,WANG Yingjun,WU Gang,Synthesis and properties of degradable medical polyurethane with L-Lysine ethyl ester diisocyanate as hard segment,Polymer Materials Science and Engineering,29,22(2013)(何显运,王迎军,吴刚,功能性LDI硬段医用可降解聚氨酯的合成与性能,高分子材料科学与工程,29,22(2013)) |
| [9] | Lee,J.B.,Lee,S.H.,Yu,S.M.,Park,J.-C.,Choi,J.B.,and Kim,J.K.,PLGA scaffold incorporated with hydroxyapatite for cartilage regeneration,Surface and Coatings Technology,202,5757 (2008) |
| [10] | Grad,S.,Kupcsik,L.,Goma,K.,Gogolewski,S.,Alini,M.,The use of biodegradable polyurethane scaffolds for cartilage tissue engineering:potential and limitations,Biomaterials,24,5163(2003) |
| [11] | Witte van de,P.,Dijkstra,P.J.,Berg van den,J.W.A.,Feijen,J.,Phase separation processes in polymer solutions in relation to membrane formation,Journal of Membrane Science,117,1(1996) |
| [12] | Heijkants,R.G.J.C.,van Calck,R.V.,van Tienen,T.G.,de Groot,J.H.,Pennings,A.J.,Buma,P.,Veth,R.P.H.,Schouten,A.J.,Polyurethane scaffold formation via a combination of salt leaching and thermally induced phase separation,Journal of Biomedical Materials Research Part A,87A,921 (2008) |
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