采用溶剂挥发法,以乳酸羟基乙酸共聚物(PLGA)制备了载牛血清白蛋白(BSA)的PLGA纳米粒(PLGA NP),采用两种修饰方式(直接吸附法和共价交联法)以壳聚糖(chitosan,CS)修饰纳米粒表面,通过考察修饰方法对纳米粒的理化性质、释药性质以及对BSA构型的影响,吸附法修饰的纳米粒(ADCS NP)包封率提高,达到(87.8±3.1)%,突释效应显著;共价交联法修饰的纳米粒(CBCS NP)包封率降低,释药速率增加.3组纳米粒释放速率顺序为V(CBCS NP)>V(ADCS NP)>V(PLGA NP).PLGA NP与ADCS NP包载的BSA构象未见明显改变,而CBCS NP包载的BSA的圆二色谱中α-螺旋含量降低,β-折叠增加.因此,ADCS NP更适合作为蛋白多肽类药物载体.
参考文献
| [1] | 由英才,伍国琳,李鑫,朱常英,焦京亮,李桢,沈忻.生物降解多肽和蛋白质药物的控制释放研究[J].南开大学学报(自然科学版),2002(01):99-103. |
| [2] | Kang SW;Lim HW;Seo SW;Jeon O;Lee M;Kim BS .Nanosphere-mediated delivery of vascular endothelial growth factor gene for therapeutic angiogenesis in mouse ischemic limbs.[J].Biomaterials,2008(8):1109-1117. |
| [3] | Sakai T;Yamamoto H;Tahara K et al.[J].International Journal of Pharmaceutics,2008,354:210-216. |
| [4] | Sengupta S;Eavarone D;Capilal I et al.[J].Nature,1794,436(28):568-572. |
| [5] | Fischer S;Uetz-von-Allmen E;Waeckerle-Men Y;Groettrup M;Merkle HP;Gander B .The preservation of phenotype and functionality of dendritic cells upon phagocytosis of polyelectrolyte-coated PLGA microparticles.[J].Biomaterials,2007(6):994-1004. |
| [6] | Jaganathan KS;Vyas SP .Strong systemic and mucosal immune responses to surface-modified PLGA microspheres containing recombinant Hepatitis B antigen administered intranasally[J].Vaccine,2006(19):4201-4211. |
| [7] | Munier S;Messai I;Delair T;Verrier B;Ataman-Onal Y .Cationic PLA nanoparticles for DNA delivery: Comparison of three surface polycations for DNA binding, protection and transfection properties[J].Colloids and Surfaces, B. Biointerfaces,2005(3/4):163-173. |
| [8] | Yamamoto H;Kuno Y;Sugimoto S;Takeuchi H;Kawashima Y .Surface-modified PLGA nanosphere with chitosan improved pulmonary delivery of calcitonin by mucoadhesion and opening of the intercellular tight junctions[J].Journal of Controlled Release: Official Journal of the Controlled Release Society,2005(2):373-381. |
| [9] | Wang XH.;Li DP.;Wang WJ.;Feng QL.;Cui FZ.;Xu YX.;Song XH. .Covalent immobilization of chitosan and heparin on PLGA surface[J].International Journal of Biological Macromolecules: Structure, Function and Interactions,2003(1/3):95-100. |
| [10] | 邵丽,邓阳全,吴旭,杨银,张志斌.载药壳聚糖缓释微球的制备及其释放研究[J].功能材料,2009(06):959-961,965. |
| [11] | Chen, HL;Yang, WZ;Chen, H;Liu, LR;Gao, FP;Yang, XD;Jiang, Q;Zhang, QQ;Wang, YS .Surface modification of Mitoxantrone-loaded PLGA nanospheres with chitosan[J].Colloids and Surfaces, B. Biointerfaces,2009(2):212-218. |
| [12] | 官习鹏,全大萍,廖凯荣,麦堪成.壳聚糖修饰PLGA阳离子型纳米微球的制备与表征[J].高等学校化学学报,2006(10):1965-1968. |
| [13] | 张静,张政朴,宋瑜,贺秉坤.染料壳聚糖微球的制备及其对牛血清白蛋白（BSA）吸附性能的研究[J].高等学校化学学报,2005(12):2363-2368. |
| [14] | 张奕,曾戎,周长忍,何柱国,梁志红.基于QCM-D的天然多糖材料的蛋白吸附研究[J].功能材料,2009(02):287-290. |
| [15] | Nishikawa T;Akiyoshi K;Sunamoto J .MACROMOLECULAR COMPLEXATION BETWEEN BOVINE SERUM ALBUMIN AND THE SELF-ASSEMBLED HYDROGEL NANOPARTICLE OF HYDROPHOBIZED POLYSACCHARIDES[J].Journal of the American Chemical Society,1996(26):6110-6115. |
| [16] | 张杰,张新宇,张代佳,修志龙.喷雾法制备载牛血清白蛋白海藻酸钙微球系统研究[J].大连理工大学学报,2009(06):822-826. |
| [17] | Wang Y S;Jiang Q;Liu L L et al.[J].Polymer,2007,48:4153-4142. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%