采用改进的Hummers 法制备氧化石墨烯，并用红外、拉曼光谱和扫描电镜等手段对氧化石墨烯的结构及形貌进行表征，考察了不同溶剂对L-色氨酸荧光光谱的影响；分别以L-色氨酸、氧化石墨烯为荧光剂和荧光猝灭剂，借助荧光光谱法研究了氧化石墨烯对L-色氨酸的荧光猝灭作用。根据紫外吸收光谱、Stern-Volmer方程和Lineweawer-Burk双倒数曲线方程，得出了氧化石墨烯对L-色氨酸荧光猝灭类型、猝灭速率常数( kq )和结合常数( K)等参数。结果表明，氧化石墨烯对L-色氨酸的荧光有较强的猝灭作用，其荧光猝灭机理是氧化石墨烯与L-色氨酸形成复合物的静态猝灭。
Graphene oxide ( GO) was prepared by the modified Hummers method and used as a fluorescence quenching reagent of L-tryptophan ( L-Trp) . The structure of GO was characterized by FT-IR and Raman spectroscopy. The binding mechanism of L-Trp on GO and their reaction were investigated by UV spectroscopy and the fluorescence method. Results show that GO has a strong flu-orescence quenching ability for L-Trp. The quenching efficiency is 95. 6% for L-Trp with a concentration of 2×10-5 mol·L-1 when the GO concentration is 16×10-5 g·mL-1. The binding constants of L-Trp on GO were 2. 03×105, 1. 83×105 and 1. 63×105 mL·g-1 at 25, 30 and 35℃, respectively, as revealed by the Lineweawer-Burk equation, indicating a strong interaction between GO and L-Trp. GO quenches the intrinsic fluorescence of L-Trp by the formation of a complex, and is a static quenching mechanism. The quenching rate constant was 6. 65×1012 mL·(g·s)-1 at 25 ℃ as determined by the Stern-Volmer equation.
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