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  4. 强迫流动环境对蛋白质结晶的影响

材料导报, 2012, 26(21): 70-74.

强迫流动环境对蛋白质结晶的影响

鹿芹芹 1, , 陈瑞卿 2, , 解旭卓 3, , 尹大川 4,

1.西北工业大学生命学院,空间生物实验模拟技术国防重点学科实验室,西安710072

2.西北工业大学生命学院,空间生物实验模拟技术国防重点学科实验室,西安710072

3.西北工业大学生命学院,空间生物实验模拟技术国防重点学科实验室,西安710072

4.西北工业大学生命学院,空间生物实验模拟技术国防重点学科实验室,西安710072

基金项目:   国家高等学校博士学科点专项科研基金(20116102120052)   西北工业大学基础研究基金(JC201127)   国家自然科学基金(31170816)  

关键词： 蛋白质结晶 , 强迫流动环境 , 晶体质量

Effect of Forced Flow Environment on Protein Crystallization

生长高质量蛋白质晶体一直是结构生物学领域的瓶颈问题之一.因此,研究蛋白质结晶方法学,提高获得高质量蛋白质晶体的几率,意义重大.强迫流动作为一种特殊的物理环境,对蛋白质溶液形核以及晶体生长过程有着显著的影响.综述了不同的强迫流动环境对蛋白质结晶过程的影响,并对影响机理进行了分析.近期的研究发现,对某些蛋白质而言,强迫流动环境有利于生长尺寸大、质量高的蛋白质晶体.

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