以海藻酸钠和γ-氨丙基三乙氧基硅烷为原料,在一定条件下使二者发生交联反应生成复合水凝胶,并以此复合凝胶作为固定化纤维素酶的载体.纤维素酶的包埋率超过了85%,酶固定前后的SEM图表明纤维素酶非常均匀地分布在载体中.探讨了pH、温度对固定化酶和游离酶活力的影响,结果表明固定化酶具有对pH和温度更高的稳定性,固定化酶的最适宜pH为3.6,最佳催化温度为50℃.通过Michaelis-Menten(米氏)方程计算得到的固定化酶的米式常数(Km)值较游离酶大,表明固定化酶的可重复使用性和储藏稳定性良好.连续使用10次后依然保留＞50%酶活力,储藏1个月后固定化酶依然有81%的相对酶活力.
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