以大肠杆菌和金黄色葡萄球菌做模型检测抗菌活性,选择L-929细胞通过MTT法评价毒性.结果证明纳米银具有显著的抗菌活性且呈剂量依赖性;不论在液体培养基还是固体培养基,30×10<'-6>浓度产生的抗菌作用大于50%,但有潜在的细胞毒性;即使在3×10<'-6>浓度下.细胞毒性达到2级,有26%,～50%的细胞生长受到抑制.
E. coil and S. aureus were used as model for testing antimicrobial activity, and L-929 cell line was selected for toxicity evaluation by cellular mitochondrial function(MTT assay). The results show that silver nanoparticles(Ag NPs)can significantly in-crease antibacterial activity with the increasing of concentration of the silver nanoparticles. The lowest effective concentration of silver nanoparticles(30 X 10<'-6>)which leads to more than 50% of inhibition in liquid medium and agar plate on bacterias is still satis-factory but will produce potential cytotoxicity. Even at 3 x 10<'-6> concentration of silver nanoparticles the cytotoxicity still exists with 2grade which represents 26%-50% of cell growth inhibited, and 30×10<'-6> displays similar impact.
参考文献
| [1] | 徐光年,乔学亮,邱小林,陈建国.单分散球形纳米银粒子的制备新方法及其抗菌性能[J].稀有金属材料与工程,2008(09):1669-1672. |
| [2] | Mahendra R;Alka Y;Aniket CL .[J].Biotechnology Advances,2009,27:76. |
| [3] | Stephan T D;Panittamat K;Pranut P .[J].Colloids and Surfaces A:Physicochemical and Engineering gspects,2006,289(1-3):105. |
| [4] | Perelshtein, I;Applerot, G;Perkas, N;Wehrschuetz-Sigl, E;Hasmann, A;Guebitz, G;Gedanken, A .CuO-cotton nanocomposite: Formation, morphology, and antibacterial activity[J].Surface & Coatings Technology,2009(1/2):54-57. |
| [5] | Yang Hulling;Lin Chunte;Huang Chihpin .[J].Water Research,2009,43:3777. |
| [6] | Michael S;Nathalie S;Hans K et al.[J].Appetite,2008,51(02):283. |
| [7] | Shia Zhilong;Neoha K G;Kanga E T et al.[J].Biomaterials,2006,27:2440. |
| [8] | Kumar A;Vemula PK;Ajayan PM;John G .Silver-nanoparticle-embedded antimicrobial paints based on vegetable oil[J].Nature materials,2008(3):236-241. |
| [9] | Li Qilin;Shaily M;Deliria Y L et al.[J].Water Research,2008,42(18):4591. |
| [10] | Chen X;Schluesener HJ .Nanosilver: A nanoproduct in medical application.[J].Toxicology Letters: An International Journal Providing a Forum for Original and Pertinent Contributions in Toxicology Research,2008(1):1-12. |
| [11] | Xu Xiaoyi;Yang Qingbiao;Wang Yongzhi et al.[J].European Polymer Journal,2006,42:2081. |
| [12] | Rahmana M F;Wanga J;Pattersona T A et al.[J].Toxicology Letters,2009,187(I):15. |
| [13] | Kalimuthu K;Elayappan B;SureshBabu R K P et al.[J].Colloids and Surfaces B:Biointerfaces,2009,73:51. |
| [14] | Silver S;Phung le T;Silver G .Silver as biocides in burn and wound dressings and bacterial resistance to silver compounds[J].Journal of industrial microbiology & biotechnology,2006(7):627-634. |
| [15] | Ian C .[J].Journal of Antimicrobial Chemotherapy,2007,59:587. |
| [16] | Petica A;Gavriliu S;Lungua M et al.[J].Materials Science and Engineering B:Solid-state materials for advanced technology,2008,152:22. |
| [17] | Kim Junsung;Eunye Kuk;Yu Kyeongnam et al.[J].Nanomedicine-Nanotechnology Biology and Medicine,2007,3:95. |
| [18] | Brigita T;Barbara S;Boris O et al.[J].Carbohydrate Polymers,2009,75:618. |
| [19] | Jose L E;Justin L B;Jose R M et al.[J].Journal of Nanobiotechnology,2005,3(06):1. |
| [20] | Tiena Derchi;Tsengb Kuohsiung;Liaob Chihyu et al.[J].Journal of Alloys and Compounds,2009,473(1-2):298. |
| [21] | Lin Weisheng;Huang Yuewern;Zhou Xiaodong et al.[J].Toxicology and Applied Pharmacology,2006,217:252. |
| [22] | Hussain SM;Hess KL;Gearhart JM;Geiss KT;Schlager JJ .In vitro toxicity of nanoparticles in BRL 3A rat liver cells.[J].Toxicology in vitro: an international journal published in association with BIBRA,2005(7):975-983. |
| [23] | Burd A;Kwok C H;Hung S C et al.[J].Wound Repair and Regeneration,2007,15:94. |
| [24] | Lu Lv;Yiqun Luo;Wun Jern Ng .Bactericidal activity of silver nanoparticles supported on microporous titanosilicate ETS-10[J].Microporous and Mesoporous Materials,2009(3):304-309. |
| [25] | Alma Sugjoon;Leea Shinjae;Kookb Joungki et al.[J].Dental Materials,2009,25:206. |
| [26] | Luung Nguyendang;Lee Youngkwan;Nam Jaedo .[J].European Polymer Journal,2008,44:116. |
| [27] | Silver S. .Bacterial silver resistance: molecular biology and uses and misuses of silver compounds [Review][J].FEMS Microbiology Reviews,2003(2/3):341-353. |
| [28] | WU Xue-ling,QIU Guan-zhou,GAO Jian,DING Jian-nan,KANG Jian,LIU Xin-xing.Mutagenic breeding of silver-resistant Acidithiobacillus ferrooxidans and exploration of resistant mechanism[J].中国有色金属学会会刊（英文版）,2007(02):412-417. |
| [29] | Lok Chunnam;Ho Chiming;Chen Rong et al.[J].Journal of Biological Inorganic Chemistry,2007,12:527. |
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