制备了PU/n-HA/PA66股骨髁,用扫描电镜观察材料表面情况并测定其孔隙率；将PU/n-HA/PA66股骨髁与自体髁植入犬股骨远端,以替代、修复骨软骨缺损,进行大体观察、组织学、免疫组化、CT、血常规和生化检测以及肝、肾、脾组织学检测.结果表明材料孔隙率为80.89％±5.01％,孔径主要分布在300～800μm之间.术后实验动物活动正常,切口愈合良好,两组髁假体均与自体骨结合紧密,PU/n-HA/PA66股骨髁网孔中的骨小梁逐渐增多成熟,材料孔穴中的新生骨Ⅰ型胶原阳性表达.术后动物碱性磷酸酶水平升高[(62.67±24.04) U/L],肝、脾、肾HE染色未见异常.PU/n-HA/PA66股骨髁具有良好的骨修复、软骨替代能力和生物相容性,具有应用前景.
参考文献
| [1] | Vukasovi(c) A;Ivkovic A;Jezek D et al.Histologic assess-ment of tissue healing of hyaline cartilage by use of semi-quantitative evaluation scale[J].Acta Medica Croatica,2011,65(03):227-235. |
| [2] | Angel MJ;Razzano P;Grande DA .Defining the challenge. The basic science of articular cartilage repair and response to injury[J].Sports medicine and arthroscopy review,2003(3):168-181. |
| [3] | Klein TJ;Malda J;Sah RL;Hutmacher DW .Tissue engineering of articular cartilage with biomimetic zones.[J].Tissue engineering, Part B. Reviews,2009(2):143-157. |
| [4] | Martin I;Miot S;Barbero A;Jakob M;Wendt D .Osteochondral tissue engineering.[J].Journal of Biomechanics,2007(4):750-765. |
| [5] | Li Y B;Wijn J;Klein C P A T et al.Preparation and characterization of nanograde osteoapatite-like rod crystals[J].Journal of Materials Science:Materials in Medicine,1994,5:252-255. |
| [6] | Li Y B;Zuo Y;Wei J .Preparation of nano-hydroxyapa-tite/polyamide composite biomaterial[P].China:CNZL200310111033.5,2003-10-11. |
| [7] | 左奕,李玉宝,魏杰,韩劲,许凤兰.n-HA/PA系列生物医用复合材料的制备与表征[J].功能材料,2004(04):513-516. |
| [8] | 刘浩怀,张利,邹琴,左奕,李吉东,李玉宝.聚氨酯软骨-骨双层复合材料的构建及性能研究[J].功能材料,2010(04):652-655. |
| [9] | Hou Q;Grijpma DW;Feijen J .Porous polymeric structures for tissue engineering prepared by a coagulation, compression moulding and salt leaching technique.[J].Biomaterials,2003(11):1937-1947. |
| [10] | 董志红,李玉宝,张利,左奕.软骨修复用HA/PU多孔支架材料的制备与表征[J].无机材料学报,2007(06):1255-1258. |
| [11] | 王立,左奕,邹琴,李玉宝.组分对含羟基磷灰石/脂肪族聚氨酯复合骨组织工程支架的理化性能及生物学性能的影响[J].高等学校化学学报,2011(10):2453-2459. |
| [12] | Kavlock KD;Pechar TW;Hollinger JO;Guelcher SA;Goldstein AS .Synthesis and characterization of segmented poly(esterurethane urea) elastomers for bone tissue engineering.[J].Acta biomaterialia,2007(4):475-484. |
| [13] | Verdonk,P.;Beaufils,P.;Bellemans,J.;Djian,P.;Heinrichs,E.-L.;Huysse,W.;Laprell,H.;Siebold,R.;Verdonk,R. .Successful treatment of painful irreparable partial meniscal defects with a polyurethane scaffold: Two-year safety and clinical outcomes[J].American Journal of Sports Medicine,2012(4):844-853. |
| [14] | Mauth C;Bono E;Haas S et al.Cell-seeded polyure-thane-fibrin structures-a possible system for intervertebral disc regeneration[J].European Cells and Materials,2009,2(18):27-38. |
| [15] | Jose Abel de la Pe?a-Salcedo;Miguel Angel Soto-Miranda;Jose Fernando Lopez-Salguero .Back to the future: a 15-year experience with polyurethane foam-covered breast implants using the partial-subfascial technique.[J].Aesthetic plastic surgery,2012(2):331-338. |
| [16] | 傅明源;孙酣经.聚氨酯弹性体及应用[M].北京:化学工业出版社,1999:27-29. |
| [17] | 郑振辉;周淑佩;彭双清.实用医学实验动物学[M].北京:北京大学医学出版社,2008:429-430. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%