利用反相悬浮交联法制备了壳聚糖基质,基质再与环氧丙基三甲基氯化铵反应,制得强碱性的壳聚糖阴离子分离介质.研究了分离介质的最佳制备条件,测定了其最大阴离子交换容量和滴定曲线,最后用5′-鸟苷酸对其进行交换性能表征.结果表明,最佳制备工艺为:以1.0％(m/V)的NaOH为催化剂,1.0g壳聚糖基质与5.0mL环氧丙基三甲基氯化铵于50℃反应3.0h,产物为强碱性阴离子分离介质,其最大交换容量为2.88mmol/g干介质,交换吸附结果表明对5′-鸟苷酸最大吸附量为98.59mg/g干介质.
参考文献
| [1] | Gao B Y;Xu X;Wang Y et al.Preparation and characteristics of quaternary amino anion exchanger from wheat residue[J].Journal of Hazardous Materials B:Environmental Technologies,2009,165(1-3):461. |
| [2] | Yoshida H;Nishihara H;Kataoka T .Adsorption of BSA on QAE-dextran:Equilibrium[J].Biotechnology and Bioengineering,1993,41(02):280. |
| [3] | Muzzarelli R A A.Chitin[M].New York:pergaman Press,1977 |
| [4] | Bai P L;Cao F M;Lan X R et al.Chitosan gel beads immobilized Cu(Ⅱ) for selective adsorption of amino acids[J].Journal of Biochemical and Biophysical Methods,2008,70(06):903. |
| [5] | 张静,张政朴,贺秉坤.染料壳聚糖微球的制备及其对人血清白蛋白吸附性能研究[J].高分子学报,2006(04):340-344. |
| [6] | E.Guibal .Heterogeneous catalysis on chitosan-based materials:a review[J].Progress in Polymer Science,2005(1):71-109. |
| [7] | Huang XJ;Wang QQ;Huang BL .Preparation and evaluation of stable coating for capillary electrophoresis using coupled chitosan as coated modifier[J].Talanta: The International Journal of Pure and Applied Analytical Chemistry,2006(2):463-468. |
| [8] | X.D.Liu;N.Nishi;N.Sakairi;S.Tokura .A novel method for immobilization of chitosan onto nonporous glass beads through a 1,3-thiazolidine linker[J].Polymer: The International Journal for the Science and Technology of Polymers,2003(4):1021-1026. |
| [9] | Q.-H.Shi;Y.Tian;X.-Y.Dong;S.Bai;Y.Sun .Chitosan-coated silica beads as immobilized metal affinity support for protein adsorption[J].Biochemical Engineering Journal,2003(3):317-322. |
| [10] | 陈飞,骆广生,王玉军.改性壳聚糖树脂对利尿剂的吸附性能[J].高分子学报,2005(01):53-59. |
| [11] | Zhang L N;Zhou J P;Yang G et al.Preparative fractionation of polysaccharides by columns packed with regenerated cellulose[J].Journal of Chromatography A,1998,816(02):131. |
| [12] | 霍小敏,屠春燕,宋慧敏,欧阳平凯.HPLC法测定酵母RNA降解的4种核苷酸[J].南京工业大学学报(自然科学版),2002(04):61-64. |
| [13] | 蔡照胜,王锦堂,杨春生,许琦,严金龙.2-羟丙基三甲基氯化铵壳聚糖的制备及其表征[J].精细化工,2004(09):655-657,673. |
| [14] | 顾觉奋.分离纯化工艺原理[M].北京:中国医药科技出版社,2002:108. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%