利用EDTA作为捕获载体,结合场放大进样技术,建立了一种基于场放大进样-电动反平衡扫集在线双重富集的毛细管电泳富集分离新体系并用于铁矿石中锰、铬、铅、铜、镍和钴6种重金属元素的检测.金属离子首先通过场放大进样堆积在水塞与高电导缓冲溶液的界面上,然后被迎面而来的带负电荷的EDTA捕获,样品区带被进一步压缩,实现信号的双重放大.通过控制电渗流使金属-EDTA螯合物处于准静止状态,实现大体积进样及检测信号的高度放大.通过15 kV电动进样50 min双重富集后,灵敏度比常规毛细管电泳提高了1.2×104～8.5×104倍,铁矿石中6种重金属离子的检出限为0.06～0.32 μg/L.用建立的方法测定铁矿石中锰、铬、铅、铜、镍和钴,测定值与ICP-MS法测定值之间的相对误差在5.3％～11.5％之间.
参考文献
| [1] | Felipe-Sotelo M.;Andrade JM.;Carlosena A.;Prada D. .Partial least squares multivariate regression as an alternative to handle interferences of Fe on the determination of trace Cr in water by electrothermal atomic absorption spectrometry[J].Analytical chemistry,2003(19):5254-5261. |
| [2] | Kumar B N;Ramana D K V;Harinath Y et al.Separation and preconcentration of Cd (Ⅱ),Cu (Ⅱ),Ni (Ⅱ) and Pb(Ⅱ) in water and food samples using amberlite XAD-2 functionalized with 3-(2-Nitrophenyl)1H-1,2,4-triazole-5 (4H)-thione and determination by inductively coupled plasma atomic emission spectrometry[J].Journal of Agricultural and Food Chemistry,2011,59(20):11352-11358. |
| [3] | Xiangbao Long;Manuel Miro;Elo Harald Hansen;Jose Manuel Estela;Victor Cerda .Hyphenating Multisyringe Flow Injection Lab-on-Valve Analysis with Atomic Fluorescence Spectrometry for On-Line Bead Injection Preconcentration and Determination of Trace Levels of Hydride-Forming Elements in Environmental Samples[J].Analytical chemistry,2006(24):8290-8298. |
| [4] | Spiros A. Pergantis;Tammy L. Jones-Lepp;Edward M. Heithmar .Hydrodynamic Chromatography Online with Single Particle-Inductively Coupled Plasma Mass Spectrometry for Ultratrace Detection of Metal-Containing Nanoparticles[J].Analytical chemistry,2012(15):6454-6462. |
| [5] | 武映梅,石仕平,宋武元.X射线荧光光谱粉末压片法检测合金铸铁中13种成分[J].冶金分析,2012(07):32-37. |
| [6] | 周煜,谭艳山,朱利亚,牛春林,罗春华,李玉仙,万仲建,王前会.过氧化钠熔融-硫氰酸铵差示光度法测定钼精矿和钼焙砂中钼[J].冶金分析,2012(09):68-72. |
| [7] | Richard F.Carbonaro;Alan T.Stone .Speciation of Chromium(lll)and Cobalt(lll)(Amino)carboxylate Complexes Using Capillary Electrophoresis[J].Analytical chemistry,2005(1):155-164. |
| [8] | Almeda, S;Gandolfi, HE;Arce, L;Valcarcel, M .Potential of porphyrins as chromogenic reagents for determining metals in capillary electrophoresis[J].Journal of chromatography, A: Including electrophoresis and other separation methods,2009(34):6256-6258. |
| [9] | Joselito P. Quirino;Shigeru Terabe .Approaching a Million-Fold Sensitivity Increase in Capillary Electrophoresis with Direct Ultraviolet Detection: Cation-Selective Exhaustive Injection and Sweeping[J].Analytical chemistry,2000(5):1023-1030. |
| [10] | Isoo K.;Terabe S. .Analysis of metal ions by sweeping via dynamic complexation and cation-selective exhaustive injection in capillary electrophoresis[J].Analytical chemistry,2003(24):6789-6798. |
| [11] | Na Yan;Lei Zhou;Zaifang Zhu;Huige Zhang;Ximin Zhou;Xingguo Chen .Constant pressure-assisted head-column field-amplified sample injection in combination with in-capillary derivatization for enhancing the sensitivity of capillary electrophoresis[J].Journal of chromatography, A: Including electrophoresis and other separation methods,2009(20):4517-4523. |
| [12] | Wang, Z.;Liu, C.;Kang, J. .A highly sensitive method for enantioseparation of fenoprofen and amino acid derivatives by capillary electrophoresis with on-line sample preconcentration[J].Journal of chromatography, A: Including electrophoresis and other separation methods,2011(13):1775-1779. |
| [13] | Farid Oukacine;Joselito P. Quirino;Laurent Garrelly;Bernard Romestand;Tao Zou;Herve Cottet .Simultaneous Electrokinetic and Hydrodynamic Injection for High Sensitivity Bacteria Analysis in Capillary Electrophoresis[J].Analytical chemistry,2011(12):4949-4954. |
| [14] | Yueju Wang;Bryan R. Fonslow;Catherine C. L. Wong;Aleksey Nakorchevsky;John R. Yates .Improving the Comprehensiveness and Sensitivity of Sheathless Capillary Electrophoresis-Tandem Mass Spectrometry for Proteomic Analysis[J].Analytical chemistry,2012(20):8505-8513. |
| [15] | Zhang X;Zhang Z .Sweeping under controlled electroosmotic flow and micellar electrokinetic chromatography for on-line concentration and determination of trace phlorizin and quercitrin in urine samples.[J].Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis,2011(2):330-335. |
| [16] | Zhongqi Xu;Kentaro Nakamura;Andrei R. Timerbaev;Takeshi Hirokawa .Another Approach Toward over 100 000-Fold Sensitivity Increase in Capillary Electrophoresis: Electrokinetic Supercharging with Optimized Sample Injection[J].Analytical chemistry,2011(1):398-401. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%