用共价键合法将青霉素酰化酶固定化在珠状多孔的甲基丙烯酸缩水甘油酯(GM)共聚物上,研究了固定化反应时间、温度、pH值和酶液用量对固定化青霉素酰化酶的表观活性、表观偶联效率、活性回收及稳定性的影响. 将GM共聚物载体加入到磷酸缓冲液(0.1 mol/L, pH 10.8)与青霉素酰化酶液(每克干载体用酶液1 ml)的混合溶液中,在30 ℃下反应72 h,单位质量(干重)固定化酶的表观活性为348 U/g,表观偶联效率为66.7%,活性回收为31.7%.
参考文献
| [1] | Bahulekar R V;Prabhune A A;SivaRaman H;Ponrathnam S .[J].Polymer,1993,34(01):163. |
| [2] | Fonseca L P;Cardoso J P;Cabral J M S .[J].Journal of Chemical Technology and Biotechnology,1993,58(01):27. |
| [3] | Bianchi D;Golini P;Bortolo R;Cesti P .[J].Enzyme and Microbial Technology,1996,18(07):592. |
| [4] | 乌云高娃,卢冠忠.6-APA合成反应中固定化青霉素酰化酶载体及固定化研究进展[J].分子催化,2002(01):75-80. |
| [5] | Kotha A;Raman R C;Ponrathnam S;Kumar K K,Shewale J G .[J].Reactive and Functional Polymers,1996,28(03):235. |
| [6] | Kotha A.;Ponrathnam S.;Kumar KK.;Shewale JG.;Raman RC. .Beaded reactive polymers 3 - Effect of triacrylates as crosslinkers on the physical properties of glycidyl methacrylate copolymers and immobilization of penicillin G acylase[J].Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology,1998(3):191-203. |
| [7] | 李晓芬,何静,马润宇.青霉素酰化酶在介孔分子筛MCM-41上的固定化研究[J].化学学报,2000(02):167. |
| [8] | 乌云高娃,卢冠忠,郭杨龙,王筠松,魏东芝.大孔聚甲基丙烯酸缩水甘油酯固定化酶载体的合成及性能研究[J].化学学报,2002(03):504-508. |
| [9] | 乌云高娃,卢冠忠,郭杨龙,王筠松,魏东芝.聚甲基丙烯酸缩水甘油酯固定化青霉素酰化酶性质的研究[J].华东理工大学学报(自然科学版),2002(06):633-636. |
| [10] | Sundberg L;Porath J .[J].Journal of Chromatography,1974,90(01):87. |
| [11] | Bryjak J;Trochimczuk A;Noworyta A .[J].Journal of Chemical Technology and Biotechnology,1993,57(01):73. |
| [12] | Bahulekar R V;Ponrathnam S;Ayyangar N R;Kumar K K,Shewale J G .[J].Journal of Applied Polymer Science,1992,45(02):279. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%