羟基磷灰石涂层改善SLA-钛表面形貌及促进成骨细胞附着

稀有金属材料与工程, 2015, 44(1): 67-72.

羟基磷灰石涂层改善SLA-钛表面形貌及促进成骨细胞附着

孙旭 ^1, , 杨晓喻 ^2, , 苏源 ^3, , 滕敏华 ^4, , 廖健 ^5, , 牛爱迪 ^6, , 梁星 ^7,

1.四川大学华西口腔医院,口腔疾病研究国家重点实验室,四川成都610041

2.南方医科大学附属口腔医院,广东省口腔医院,广东广州510280

3.四川大学华西口腔医院,口腔疾病研究国家重点实验室,四川成都610041

4.四川大学华西口腔医院,口腔疾病研究国家重点实验室,四川成都610041

5.贵阳医学院附属医院,贵州贵阳550000

6.四川大学华西口腔医院,口腔疾病研究国家重点实验室,四川成都610041

7.四川大学华西口腔医院,口腔疾病研究国家重点实验室,四川成都610041

基金项目: National Natural Science Foundation of China (30672345,30271428) Sichuan Science and Technology Support Program (2009SZ0070)

关键词：钛 , SLA-Ti表面 , 羟基磷灰石(HA) , 蛋白吸附 , 成骨细胞附着

Morphology Improvement of Sandblasted and Acid-Etched Titanium Surface and Osteoblast Attachment Promotion by Hydroxyapatite Coating

Keywords： titanium , SLA-Ti surface , hydroxyapatite (HA) , protein adsorption , osteoblast attachment

喷砂结合酸洗技术(SLA)和羟基磷灰石(HA)沉积技术是2种广泛用于改善钛种植体表面特征的方法.本研究中,用一种简单的沉积方法将一层HA沉积在SLA处理的钛表面(SLA-Ti).应用扫描电子显微镜(SEM)和原子力显微镜(AFM)观察SLA-Ti和HA覆盖的SLA-钛(HA-Ti)的表面形貌和测量表面粗糙度.同时对2种钛表面的蛋白质吸附,成骨细胞趋化和成骨细胞附着进行分析.结果表明:SLA-Ti表面布满大量规则的微孔,HA-Ti表面在多微孔的背景下覆盖大量规则的微颗粒.与SLA-Ti相比,HA-Ti表面的粗糙度较低.2种钛表面的蛋白吸附均连续均匀,成骨细胞趋化也无差异.在试件培养12和24 h后,发现HA-Ti表面附着的成骨细胞及成骨细胞间联系比SLA-Ti表面更多.这些研究表明HA涂层改善了SLA-Ti钛的表面形貌和促进了体外成骨细胞附着.

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