采用溶液浇铸法和超声分散工艺制备了聚乳酸/纳米羟基磷灰石/氧化石墨烯(PLA/n-HA/GO)纳米生物复合膜,利用扫描电镜(SEM)对其断面形貌进行了研究,借助X射线衍射(XRD)和傅里叶变换红外光谱(FTIR)分析了纳米羟基磷灰石和氧化石墨烯的加入对所得复合膜结构的影响,并对其生物相容性进行了探讨.结果表明,氧化石墨烯和纳米羟基磷灰石在复合膜中分散均匀;细胞在复合膜上贴附牢固、铺展良好,具有良好的生长形态.聚乳酸/纳米羟基磷灰石/氧化石墨烯复合材料为细胞提供了接近天然细胞外基质的人造微环境,显示了该材料在引导组织修复和再生领域中的应用潜力.
参考文献
| [1] | 杨春蓉.高分子复合生物材料的研究进展[J].胶体与聚合物,2009(02):41-43. |
| [2] | Venugopal J;Molamma P;Prabhakaran et al.Biomime-tichydroxyapatite-containing composite nanofibrous substrates for bone tissue engineering[J].Philosophical Transactions of the Royal Society of London Series A Mathematical and Physical Sciences,2010,368:2065. |
| [3] | Ignjatovic N;Suljovrujic E;Stojanovic Z et al.Structure and characteristics of the hot pressed hydroxyapatite/poly-L-actide composite biomaterial[J].Science China(Chemistry),2002,34:79. |
| [4] | LeGeros R Z .Properties of osteoconductive biomaterials:Calcium phosphates[J].Clinical Orthopaedics and Related Research,2002,395:81. |
| [5] | Petite H;Viatteau V;Bensaid W et al.Tissue-engineered bone regeneration[J].Nature Am Inc,2000,18:569. |
| [6] | 任杰,周新宇.羟基磷灰石/聚乳酸及其共聚物复合生物材料[J].上海生物医学工程,2004(01):46-50,41. |
| [7] | Tetsuo Takayama;Mitsugu Todo .Improvement of mechanical properties of hydroxyapatite particle-filled poly(L-lactide) biocomposites using lysine tri-isocyanate[J].Journal of Materials Science,2009,44:5015. |
| [8] | Kricheldorf H R .Syntheses and application of polylactides[J].Chemosphere,2001,43:4954. |
| [9] | Rusu VM;Ng CH;Wilke M;Tiersch B;Fratzl P;Peter MG .Size-controlled hydroxyapatite nanoparticles as self-organized organic-inorganic composite materials.[J].Biomaterials,2005(26):5414-5426. |
| [10] | Geim AK;Novoselov KS .The rise of graphene[J].Nature materials,2007(3):183-191. |
| [11] | Gott V L;Whiffen J D;Dutten R C .Heparin bonding colloidal graphite surfaces[J].Science,1963,142:1279. |
| [12] | Adam Frank;Hammer DietrichStephan;Pfautsch Steffen;Westermann Kord .Early failure of a press-fit carbon fiber hip prosthesis with a smooth surface.[J].The Journal of arthroplasty,2002(2):217-223. |
| [13] | 马晓红,赵建国,鲍居宇.炭材料在生物医学领域的应用和进展[J].生物骨科材料与临床研究,2009(03):1-5. |
| [14] | Christel P;Menuier A S;Leclercq B .Development of a carbon-carbon hip prosthesis[J].Biomedical Materials Research,1987,21:191. |
| [15] | Pesakova V;Klezl Z;Balik K .Biomechanical and biological properties of the implant material carbon-carbon composite covered with pyrolytic carbon[J].Journal of Materials Science: Materials in Medicine,2000,12:793. |
| [16] | Xu Yuxi;Bai Hua;Lu Gewu et al.Flexible graphene films via the filtration of water-soluble noncovalent functionalized graphene sheets[J].Journal of the American Chemical Society,2008,130:5856. |
| [17] | Ignjatovic N;Savic V;Najman S;Plavgic M;Uskokovic D .A study of HAp/PLLA composite as a substitute for bone powder, using FT-IR spectroscopy.[J].Biomaterials,2001(6):571-575. |
| [18] | Wei Jie;Li Yubao;Lau Kin Tak .Preparation and characterization of a nano apatite/polyamide bioactive composite[J].Composites Part B:Eng,2007,38:301. |
| [19] | 郝杰,郑启新,郭晓东,全大萍,罗丙红.生物活性骨基质材料的研制及其细胞相容性的研究[J].生物医学工程学杂志,2005(03):433-437. |
| [20] | Liang D;Hsiao BS;Chu B .Functional electrospun nanofibrous scaffolds for biomedical applications.[J].Advanced drug delivery reviews,2007(14):1392-1412. |
| [21] | Lanza R T;Langer R.Principles of tissue engineering[M].USA:Academic Press,1996 |
| [22] | Najman S;Savic V;Djordjevic Lj;Ignjatovic N;Uskokovic D .Biological evaluation of hydroxyapatite/poly-L-lactide (HAp/PLLA) composite biomaterials with poly-L-lactide of different molecular weights intraperitoneally implanted into mice.[J].Bio-medical materials and engineering,2004(1):61-70. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%