探索一种新型聚乳酸基仿生聚合物材料的制备新方法.具体实验步骤是:利用聚乳酸上叔碳原子的自由基反应活性,在过氧化二苯甲酰的催化作用下,将马来酸酐引入聚乳酸侧链上,以此提供高反应活性的酸酐键;然后利用酸酐基团与-NH2发生N-酰化反应这一特点,将脂肪族二胺引入聚乳酸侧链上,从而克服聚乳酸降解产物的体液环境呈酸性的缺陷;再用碳二亚胺作缩合剂,在二胺改性聚乳酸材料中共价引入一种细胞粘附肽段Arg-Gly-Asp-Ser(RGDS),赋予材料生物活性和生物特异性,这样就制备了一种新型聚乳酸基仿生材料.采用MALLS、FTIR和XPS对仿生材料进行结构表征;采用罗丹明比色法、茚三酮显色法和氨基酸分析仪检测法对仿生材料中的马来酸酐、二胺和粘附肽RGDS进行定量测定.结果表明,按文中所述之制备技术,在不改变聚乳酸材料主链结构的前提下,该仿生材料中粘附肽RGDS的含量是5.12μmol/g.这就形成了一种具有类似细胞外基质的新型仿生材料.
参考文献
| [1] | Healy K E .[J].Current Opinion in Solid State and Materials Science,1999,4:381. |
| [2] | Healy K E;Rezania A;Stile R A .[J].Annals of the New York Academy of Sciences,1999,875:24. |
| [3] | Hersel U;Dahmen C;Kessler H .RGD modified polymers: biomaterials for stimulated cell adhesion and beyond.[J].Biomaterials,2003(24):4385-4415. |
| [4] | Shin h S;Jo S B;Mikos A G .[J].BIOMATERIALS,2003,24(4353) |
| [5] | Langer R;Tirrell D A .[J].Nature,2004,428:487. |
| [6] | Drotleff S;Lungwitz U;Breunig M;Dennis A;Blunk T;Tessmar J;Gopferich A .Biomimetic polymers in pharmaceutical and biomedical sciences.[J].European journal of pharmaceutics and biopharmaceutics: official journal of Arbeitsgemeinschaft fuer Pharmazeutische Verfahrenstechnik e.V,2004(2):385-407. |
| [7] | Niu X F;Wang Y L;Luo Y F et al.[J].Science Technology,2005,21(04):571. |
| [8] | Sanchez C;Arribart H;Guille MMG .Biomimetism and bioinspiration as tools for the design of innovative materials and systems[J].Nature materials,2005(4):277-288. |
| [9] | Zhang X C;McAuley K B;Goosen M F A .[J].Journal of Controlled Release,1995,34(02):175. |
| [10] | 张国栋,杨纪元,冯新德,顾忠伟.聚乳酸的研究进展[J].化学进展,2000(01):89-102. |
| [11] | Bostman O;Pihlajamaki H .Clinical biocompatibility of biodegradable orthopaedic implants for internal fixation: a review.[J].Biomaterials,2000(24):2615-2621. |
| [12] | Mehta R;Kumar V;Bhunia H;Upadhyay SN .Synthesis of poly(lactic acid): A review[J].Journal of macromolecular science.Polymer reviews,2005(4):325-349. |
| [13] | MANI R.;BHATTACHARYA M. .Functionalization of Polyesters with Maleic Anhydride by Reactive Extrusion[J].Journal of Polymer Science, Part A. Polymer Chemistry,1999(7):1693-1702. |
| [14] | Dubois P.;Narayan R. .Biodegradable compositions by reactive processing of aliphatic polyester/polysaccharide blends[J].Macromolecular symposia,2003(0):233-243. |
| [15] | Edlund U;Kallrot M;Albertsson A C .[J].Journal of the American Chemical Society,2005,127:8865. |
| [16] | Cook A D;hrkach J S;Gao N N et al.[J].Journal of Biomedical Materials Research,1997,35:513. |
| [17] | Zhu Y B;Gao C Y;Liu X Y.[J].Biomacromolecules,2002(03):1312. |
| [18] | Pan J;Wang Y L;Qin S H et al.[J].Res Part B:Appl Biomater,2005,74B:476. |
| [19] | Holmes T C .[J].Trends in Biotechnology,2002,20(01):16. |
| [20] | Kang I K;Kwon B K;Lee J H et al.[J].Biomaterials,1993,14(10):787. |
| [21] | Zhu Y B;Gao C Y;Liu X Y et al.[J].Tissue Engineering,2004,10(1-2):53. |
| [22] | 王镜岩;朱圣庚;徐长法;文镜 刘志华.生物化学[M].北京:高等教育出版社,2002:152-153. |
| [23] | Qiu K;Wan C X;Chen X et al.[J].Chinese J Biomed Eng,2003,12(01):22. |
| [24] | 赵强,万昌秀,刘建伟,邱凯.生物活性短肽RGD在PET表面接枝方法的研究[J].生物医学工程学杂志,2003(03):384-387. |
| [25] | Massia S P;Hubbell J A .[J].Journal of Cell Biology,1991,114(05):1089. |
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