壳聚糖的荧光标记是研究壳聚糖在体内的分布、降解和吸收的重要方法之一.荧光素异硫氰酸酯(FITC)是一种常用的荧光染料.本文考察了FITC与壳聚糖氨基反应时,溶液pH值、FITC溶剂以及温度对反应动力学的影响,以期建立一种快速、稳定的制备荧光标记壳聚糖的方法.结果表明:随着pH值增大,反应加快,但＞7.7时,则对反应无影响;甲醇作为FITC的溶剂时反应速度大于PBS;随着反应温度的升高,反应速度也随之加快.
参考文献
| [1] | Canh Le Tien;Monique Lacroix;Pomplilia Ispas-Szabo .Mircea-Alexandru Mareescu N-acylated chitosan:hydrophobic macrices for controlled release[J].Journal of Controlled Release,2003,93:1-13. |
| [2] | Sundararajan V Madihally;Howard W T .Matthew, Porous Chitosan scaffolds for tissue engineering[J].Biomaterials,1999,20:1133-1142. |
| [3] | Sung Eun Kim;Jae Hynng Park;Yong Woo Cho;Hesson Chung ,Seo Young ,Jeong Eunhee ,Bae Lee Ick ,Chan Kwon .Porous chitosan scaffold containing microspheres loaded with transforming growth factor-β 1: Implications for cartilage tissue engineering[J].Journal of Controlled Release,2003,91:365-374. |
| [4] | J -K Francis Suh;Howard W T Matthew .Applications ofchitosan-based polysaccharide biomaterials in cartilage tissue engineering: a review[J].BIOMATERIALS,2000,21:2589-2598. |
| [5] | Anette Brunner;Yoshiharn Minamitake;Achim Gopferich .Labelling peptides with fluorescent probes for incorporation into degradable polymers[J].European Journal of Pharmaceutics and Biopharmaceutics,1998,45:265-273. |
| [6] | Onishi H;Machida Y .Biodegradation and distribution of water-soluble chitosan in mice.[J].Biomaterials,1999(2):175-182. |
| [7] | Roula B Qaqish;Mansoor M Amiji .Synthesis ora fluorescent chitosan derivative and its application for the study o f chitosan-mucin interactions[J].Carbohydrate Polymers,1999,38:99-107. |
| [8] | Min Huang;Zengshuan Ma;Eugene Khor .Lee-Yong Lirm Uptake of FITC-chitosan nanoparticles by A549 cells[J].Pharmaceutical Research,2002,19:1488-1494. |
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