利用100 MeV/u C离子束对高产酒精酵母菌株进行了辐照诱变的研究.采用红四氮唑作为筛选指示剂,得到了5株产酒能力有所提高的突变酵母菌.利用甜高梁汁的发酵结果表明,T4突变菌株的产酒精能力比原始出发菌株提高了18.6%,且发酵液中的残糖含量也有所降低.随后对T4菌株在甜高粱汁中的最适宜发酵条件做了初步探索,结果表明:最适发酵温度和pH值分别为30℃和4.5.通过10 l发酵罐的验证试验表明:在同样发酵条件下,T4菌株的发酵率和产酒精能力都比原始出发菌株提高了12%.
参考文献
| [1] | Wu Dali,Hou Suiwen,Li Wenjian.Nuclear Physics Review,2008,25(3):287(in Chinese).(吴大利.侯岁稳,李文建.原子核物理评论.2008,25(3):287.) |
| [2] | Dong Xieun,Li Wenjian.Journal of Anhui Agri Sci 2008,36(12):4924 (in Chinese)(董喜存,李文建.安徽农业科学,2008,36(12):4924.) |
| [3] | Fu Qijun.Guangxi Light Industry,2001,(2):6(in Chinese).(傅其军.广西轻工业.2001,(2):6.) |
| [4] | Zhao Lixin,Zhang Yanli,Shen Fengju.Renewable Energy,2005,(4):37(in Chinese).(赵立欣,张艳丽,沈丰菊.可再生能源,2005,(4):37.) |
| [5] | Cao Junfeng,Gao Boping,Gu Weibing.Acta Agriculture Boreali-occidentalis Sinica,2006,15(3):201 (in Chinese).(曹俊峰,高博平,谷卫彬.西北农业学报,2006,16(3):201.) |
| [6] | Gnansounou E,Dauriat A,Wyman C E.Bioresource Technology,2005,96:985. |
| [7] | Bulawayo B,Bvochora J M,Muzondo M I,et al.World Journal of Microbiology & Biotechnology,1996,12:357. |
| [8] | Liu Ronghou,Li Jinxia,Shen Fei.Renewable Energy,2008,33(5):1130. |
| [9] | Wang Feng,Cheng Xiyu,Wu Tianxiang,et al.Liquor-making Science & Technology,2006,146(8):41(in Chinese).(王峰,成喜雨,吴天祥等.酿酒科技.2006,146(8):41.) |
| [10] | Chen Weiping,Tu Jin,Xiong Janhua,et al.Wine Technology,2003,(6):35(in Chinese).(陈卫平,涂谨,熊建华,张凤英.酿酒科技,2003,(6):35.) |
| [11] | Wang Furong.Fermentation Industry Analysis.Beijing:Light Industry Publishing Company of China,1980,6-22(in Chinese).(王福荣.工业发酵分析.北京:中国轻工业出版社,1980,6-22.) |
| [12] | Sun Qing,Zhao Ling,Sun Bo,et al.Renewable Energy,2005,124:18(in Chinese).(孙清,赵玲,孙波等.可再生能源.2005,124:18.) |
| [13] | Wei Zengquan.Nuclear Physics Review,200319(3):38(in Chinese).(卫增泉.原子核物理评论,2003,19(3):38.) |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%