以药物HEP为有机基质,在CaCl2、Na2 CO3常温水溶液体系中制备碳酸钙微囊.分别采用SEM、流式细胞仪和紫外分光光度计等测试技术对微囊的形貌和结构进行了观察和表征.SEM结果表明,制备的HEP/CaCO3微囊平均直径为1.5μm,球形,分散性好；紫外分光光度法检测表明,微囊具有较高的药物装载量,肝素的包封率为80％；药物体外释放实验表明,HEP/CaCO3微囊的缓释性能受pH值的影响,在pH=1的PBS溶液中有明显的释放作用,随着释放溶液中pH值的减小,微囊的缓释效果越明显.本实验方法操作简单,所制备的药物微囊装载量高,在药物控释领域具有潜在的应用价值.
参考文献
| [1] | J西尼尔;M拉多米斯基.可注射缓释制剂[M].北京:化学工业出版社,2005 |
| [2] | 许时婴;张晓鸣;夏书芹.微胶囊技术原理与应用[M].北京:化学工业出版社,2006 |
| [3] | Alexandra S.Angelatos;Kiyofumi Katagirij;Frank Caruso .Bioinspired colloidal systems via layer-by-layer assembly[J].Soft matter,2006(1):18-23. |
| [4] | Antipov A;Sukhorukov G;Leporatti S et al.Polyelectrolyte multilayer capsules permeability control[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects,2002,198(06):535. |
| [5] | Yu, AM;Wang, YJ;Barlow, E;Caruso, F .Mesoporous silica particles as templates for preparing enzyme-loaded biocompatible microcapsules[J].Advanced Materials,2005(14):1737-1741. |
| [6] | Colfen H.;Antonietti M. .Crystal design of calcium carbonate microparticles using double-hydrophilic block copolymers[J].Langmuir: The ACS Journal of Surfaces and Colloids,1998(3):582-589. |
| [7] | Limin Qi;Jie Li;Jiming Ma .Biomimetic Morphogenesis of Calcium Carbonate in Mixed Solutions of Surfactants and Double-Hydrophilic Block Copolymers[J].Advanced Materials,2002(4):300-303. |
| [8] | Inoue H;Yuasa Hashimoto N et al.Structural determination and functional analysis of a soluble matrix protein associated with calcification of the exoskeleton of the crayfish[J].Bioscience,Biotechnology and Biochemistry,2008,72(05):2697. |
| [9] | Hua A;Steven A et al.Nano-encapsulation of furosemide microcrystals for controlled drug release[J].Journal of Controlled Release,2003,86(02):59. |
| [10] | Emma L;Lisberth G et al.In vitro biocompatibility and bioactivity of microencapsulated heparin sulfate[J].Biomaterials,2007,28(08):2127. |
| [11] | Deng S G et al.A bio-inspired approach to the synthesis of CaCO3 spherical assemblies in a soluble ternary-additive system[J].Journal of Physical Chemistry B,2005,109(23):11473. |
| [12] | Shen Q;Wei H;Wang L C et al.Crystallization and aggregation behaviors of calcium carbonate in the presence of poly (vinylpyrrolidone) and sodium dodecyl-sulfate[J].Journal of Physical Chemistry B,2005,109(39):18342. |
| [13] | Wei H;Shen Q;Zhao Y;Zhou Y;Wang DJ;Xu DF .On the crystallization of calcium carbonate modulated by anionic surfactants[J].Journal of Crystal Growth,2005(3/4):439-446. |
| [14] | Wit GX;Ding J;Xue JM .Synthesis of calcium carbonate capsules in water-in-oil-in-water double emulsions[J].Journal of Materials Research,2008(1):140-149. |
| [15] | Luo XL;Qiu D;He B;Wang LJ;Luo J .Biodegradable heparin-loaded microspheres: Carrier molecular composition and microsphere structure[J].Macromolecular bioscience,2006(5):373-381. |
| [16] | Shao Yingya;Liu Xinrong;Yang Xinlin .Novel chitosan microsphere-templated microcapsules suitable for spontaneous loading of negatively charged molecules[J].Materials Science and Engineering,2009,29(03):936. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%