为了研究羟基磷灰石HA纳米微粒对低温保护剂反玻璃化结晶的影响，本文利用DSC和低温显微镜研究了含有不同粒径（20nm、40nm、60nm）和不同质量浓度（O．1％、0．2g、0．4％、0．8N）HA纳米微粒的PEG-600（50％，w／w）溶液反玻璃化过程中的结晶现象．试验结果表明：与未添加纳米微粒的PEG-600溶液相比，加入40nm、0．4％纳米微粒的HA-PEG600溶液的反玻璃化温度升高了7℃;加入20nm、0．4％和40nm、0．8％纳米微粒的HA-PEG600溶液的冰晶生长速率分别降低了35％和提高了50％；纳米低温保护剂溶液的冰晶形貌从大圆形变成了小圆形、枝晶或小圆形中夹带枝晶．
To study the effects of HA nanoparticles on devitrification of cryoprotectant solutions, The crystallization of PEG-600（50H）solutions with HA nanopartieles of different particle size（20nm.40nm. 60nm）and concentrations（0. 1%.0. 2%. 0. 4%. 0. 8%）were observed under a cryomicroscope, and vitrification parameters were measured by DSC. Experimental results showed that devitrifieation temperature of PEG-600 solution containing 0. 4H HA with the particle size of 40nm increased by 7℃, the growth rate of ice crystal of PEG-600 solution containing 0. 4% HA with the particle size of 20nm and 0. 8% HA with the particle size of 40nm decreased by 35% and increased by 50% respectively. The structure of ice crystal in PEG-600 solution with HA changed remarkably.
参考文献
| [1] | 何立群,张永锋,罗大为,程曙霞,陶乐仁,华泽钊,张爱丽,刘忠,高大勇.生命材料低温保护剂溶液二维降温结晶过程中的分形特征[J].自然科学进展,2002(11):1167-1171. |
| [2] | 李方方,刘静,乐恺.细胞尺度冰晶生长行为的相场数值模拟[J].低温物理学报,2008(02):171-175. |
| [3] | 陶乐仁,华泽钊.低温保护剂溶液结晶过程的显微实验研究[J].工程热物理学报,2001(04):481-484. |
| [4] | 叶萍,陈儿同,徐彬凯,彭承宏,沈柏用,李宏为,杜志勇,张瑞,王兆海,韩宝三.海藻酸钠/壳聚糖微囊冻存的低温显微实验[J].中国医药生物技术,2008(06):409-414. |
| [5] | 陈梅英,陈永雪,王文成,陈锦权.冷冻浓缩过程冰晶生长的相场法模拟[J].福建农林大学学报（自然科学版）,2010(05):548-551. |
| [6] | 张绍志,王葳,陈光明,王金福,项盈.低温显微装置及其对细胞胞内冰晶形成现象的观察[J].细胞生物学杂志,2003(04):231-234. |
- 下载量()
- 访问量()
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%