考察了水/离子液体两相体系中出芽短梗霉(Aureobasidium pullulans CGMCC 1244)催化 4-氯乙酰乙酸乙酯(COBE)不对称还原生成光学活性(S)-4-氯-3-羟基丁酸乙酯((S)-CHBE)的性能,并对反应条件如摇床转速、相体积比、温度、初始底物浓度和pH值等进行了优化. 结果表明,在水/1-丁基-3-甲基咪唑六氟磷酸盐体系中,出芽短梗霉催化COBE不对称还原生成(S)-CHBE, 在30 ℃, pH 6.6, 摇床转速180 r/min和不对称反应 8 h 条件下,反应物的转化率、产物ee值和浓度分别达到95.6%, 98.5%和47.1 g/L. 在控制pH值为6.6的情况下,通过分添加底物可有效提高产物(S)-CHBE浓度至75.1 g/L.
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