A novel bioactive and bioresorbabie asymmetry film was prepared. The PDLLA membrane was activated by 1, 6-hexanediamine to obtain a stable positive charge surface. Chondroitin sulfate and chitosan were then deposited on activated PDLLA membrane via layer-by-layer (LBL) electro-static assembly(ESA) technique. The deposition process was monitored by UV-Vis absorbance spectroscopy. The composite membrane was frozen lyophilized to form the asymmetry film and characterized by attenuated total reflecti( )(ATR)-FT-IR, XPS and SEM. The experimental results show that a stable 1, 6-hexanediamine layer on PDLLA substrate based on the aminolysis of the polyester and the layer thickness increase linearly first with the increase of the deposited layers, and then increases slowly due to the layer interpenetration. The test results of ATR-FT-IR and SEM show the asymmetry film is modified uniformly with a dense inner layer and a porous sponge outer layer.
参考文献
| [1] | Chen PR;Chen MH;Lin FH;Su WY .Release characteristics and bioactivity of gelatin-tricalcium phosphate membranes covalently immobilized with nerve growth factors[J].Biomaterials,2005(33):6579-6587. |
| [2] | Stang F;Wolf G;Keilhoff G .Structural parameters of collagen nerve grafts influence peripheral nerve regeneration.[J].Biomaterials,2005(16):3083-3091. |
| [3] | Haile Y;Haastert K;Cesnulevicius K;Stummeyer K;Timmer M;Berski S;Drager G;Gerardy-Schahn R;Grothe C .Culturing of glial and neuronal cells on polysialic acid.[J].Biomaterials,2007(6):1163-1173. |
| [4] | Pfister BJ;Iwata A;Taylor AG;Wolf JA;Meaney DF;Smith DH .Development of transplantable nervous tissue constructs comprised of stretch-grown axons.[J].Journal of Neuroscience Methods,2006(1):95-103. |
| [5] | Johnson EO;Zoubos AB;Soucacos PN .Regeneration and repair of peripheral nerves.[J].Injury,2005(Suppl.4):S24-29. |
| [6] | Lee DY;Choi BH;Park JH;Zhu SJ;Kim BY;Huh JY;Lee SH;Jung JH;Kim SH .Nerve regeneration with the use of a poly(l-lactide-co-glycolic acid)-coated collagen tube filled with collagen gel.[J].Journal of Cranio-Maxillofacial Surgery,2006(1):50-56. |
| [7] | Caissie R;Landry P E;Jacques L et al.Evaluation of Nerve Regeneration across a Peripheral Nerve Gap Using a Collagen Nerve Conduit Filled with a Collagen-Based Biomaterial Associated with Different Neurotrophins-Preliminary Results[J].Oral Surgery Oral Medicine Oral Pathology Oral Radiology andEndodontology,2006,101(01):43-44. |
| [8] | Bukalo O;Schachner M;Dityatev A .Modification of extracellular matrix by enzymatic removal of chondroitin sulfate and by lack of tenascin-R differentially affects several forms of synaptic plasticity in the hippocampus.[J].Neuroscience: An International Journal under the Editorial Direction of IBRO,2001(2):359-369. |
| [9] | Barkalow F J;Schwarzbauer J E .Interactions between Fibronectin and Chondroitin Sulfate Are Modulated by Molecular Context[J].Journal of Biological Chemistry,1994,269:3957-3962. |
| [10] | Bali JP;Cousse H;Neuzil E .Biochemical basis of the pharmacologic action of chondroitin sulfates on the osteoarticular system.[J].Seminars in Arthritis and Rheumatism,2001(1):58-68. |
| [11] | Suh JK;Matthew HW .Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering: a review.[J].Biomaterials,2000(24):2589-2598. |
| [12] | Shanmugasundaram N;Ravichandran P;Reddy PN;Ramamurty N;Pal S;Rao KP .Collagen-chitosan polymeric scaffolds for the in vitro culture of human epidermoid carcinoma cells.[J].Biomaterials,2001(14):1943-1951. |
| [13] | Muzzarelli R A;Mattioli-Belmonte M;Tietz C et al.Stimulatory Effect on Bone Formation Exerted by a Modified Chitosan[J].Biomaterials,1994,15:1075-1081. |
| [14] | Shanmugasundaram N;Ravichandran P;Reddy PN;Ramamurty N;Pal S;Rao KP .Collagen-chitosan polymeric scaffolds for the in vitro culture of human epidermoid carcinoma cells.[J].Biomaterials,2001(14):1943-1951. |
| [15] | Zhu H G;Ji J;Shen J C .Osteoblast Growth Promotion by Protein Electrostatic Self-Assembly on Biodegradable Poly(lactide)[J].Journal of Biomaterials Science:Polymer Edition,2005,16(06):761-774. |
| [16] | Zhengwei Mao;Lie Ma;Jie Zhou;Changyou Gao;Jiacong Shen .Bioactive Thin Film of Acidic Fibroblast Growth Factor Fabricated by Layer-by-Layer Assembly[J].Bioconjugate Chemistry,2005(5):1316-1322. |
| [17] | Fu JH;Ji J;Yuan WY;Shen JC .Construction of anti-adhesive and antibacterial multilayer films via layer-by-layer assembly of heparin and chitosan[J].Biomaterials,2005(33):6684-6692. |
| [18] | Liu YX;He T;Gao CY .Surface modification of poly(ethylene terephthalate) via hydrolysis and layer-by-layer assembly of chitosan and chondroitin sulfate to construct cytocompatible layer for human endothelial cells[J].Colloids and Surfaces, B. Biointerfaces,2005(2):117-126. |
| [19] | Ren KF;Ji J;Shen JC .Construction of polycation-based non-viral DNA nanoparticles and polyanion multilayers via layer-by-layer self-assembly[J].Macromolecular rapid communications: Publishing the newsletters of the European Polymer Federation,2005(20):1633-1638. |
| [20] | 竺亚斌,高长有,刘云肖,龚逸鸿,沈家骢.聚乳酸的层层自组装修饰及其内皮细胞相容性研究[J].高等学校化学学报,2004(07):1347-1350. |
| [21] | Chang CJ;Hsu SH .The effect of high outflow permeability in asymmetric poly(DL-lactic acid-co-glycolic acid) conduits for peripheral nerve regeneration[J].Biomaterials,2006(7):1035-1042. |
| [22] | 李青峰,徐靖宏,罗敏,干季良.不同通透性壳聚糖生物膜复合导管修复周围神经缺损的实验研究[J].上海医学,2000(07):390-392. |
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