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利用亚微米荧光示踪粒子的离焦微细观测时的衍射图样,可以分析确定荧光粒子垂直于图像平面的轴向的位置,实现对示踪粒子的三维定位.再结合应用于二维流畅测速领域的PIV技术,可利用全息荧光粒子图像测速技术实现流体速度分布的三维测量.

参考文献

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[2] Sato Y.;Inaba S.;Hishida K.;Maeda M. .Spatially averaged time-resolved particle-tracking velocimetry in microspace considering Brownian motion of submicron fluorescent particles[J].Experiments in Fluids: Experimental Methods and Their Applications to Fluid Flow,2003(2):167-177.
[3] Ovryn B. .Three-dimensional forward scattering particle image velocimetry applied to a microscopic field-of-view[J].Experiments in Fluids: Experimental Methods and Their Applications to Fluid Flow,2000(SS):S175-S184.
[4] Michael Speidel;Alexandr Jonas;Ernst-Ludwig Florin .Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imaging[J].Optics Letters,2003(2):69-71.
[5] 顾M;王桂英.共焦显微术的三维成像原理[M].北京:新时代出版社,2000
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