利用亚微米荧光示踪粒子的离焦微细观测时的衍射图样,可以分析确定荧光粒子垂直于图像平面的轴向的位置,实现对示踪粒子的三维定位.再结合应用于二维流畅测速领域的PIV技术,可利用全息荧光粒子图像测速技术实现流体速度分布的三维测量.
参考文献
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