探讨了无溶剂系统中固定化脂肪酶Novozym 435催化餐饮业废油脂转酯生产生物柴油. 反应副产物甘油可吸附在固定化酶载体表面,采用丙酮洗涤除去甘油可提高酶的稳定性. 适宜的醇/油摩尔比、酶用量、反应温度和摇床转速分别为1, 6.6 U/g, 35～40 ℃和150 r/min,不宜加水到反应体系中. 采用分步加入甲醇的方式可减轻甲醇对酶的毒害作用. 分别在反应进行到6和14 h时用丙酮除去酶表面的甘油,然后按醇/油摩尔比为1的比例加入甲醇继续反应,反应30 h后产物中的脂肪酸甲酯含量为88.6%. 连续反应300 h后,酶活性基本没有下降.
参考文献
| [1] | Soumanou MM;Bornscheuer UT .Improvement in lipase-catalyzed synthesis of fatty acid methyl esters from sunflower oil[J].Enzyme and Microbial Technology,2003(1):97-103. |
| [2] | 谭天伟,王芳,邓立,徐家立,王丽娟.生物柴油的生产和应用[J].现代化工,2002(02):4-6. |
| [3] | Nelson LA.;Marmer WN.;Foglia TA. .LIPASE-CATALYZED PRODUCTION OF BIODIESEL[J].Journal of the American Oil Chemists' Society,1996(9):1191-1195. |
| [4] | Fukuda H;Kondo A;Noda H .[J].Journal of Bioscience and Bioengineering,2001,92(05):405. |
| [5] | Kose O.;Tuter M.;Aksoy HA. .Immobilized Candida antarctica lipase-catalyzed alcoholysis of cotton seed oil in a solvent-free medium[J].Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies,2002(2):125-129. |
| [6] | Watanabe Y;Shimada Y;Sugihara A;Tominaga Y .[J].Journal of the American Oil Chemists'Society,2001,78(07):703. |
| [7] | Shimada Y;Watanabe Y;Sugihara A;Tominaga Y .[J].Journal of Molecular Catalysis B:Enzymatic,2002,17(3-5):133. |
| [8] | Lara P V;Park E Y .[J].Enzyme and Microbial Technology,2004,34(3-4):270. |
| [9] | Du W.;Xu YY.;Liu DH. .Lipase-catalysed transesterification of soya bean oil for biodiesel production during continuous batch operation[J].Biotechnology and Applied Biochemistry,2003(part 2):103-106. |
| [10] | Dossat V;Combes D;Marty A .[J].Enzyme and Microbial Technology,1999,25(3-5):194. |
| [11] | Shimada Y;Watanabe Y;Samukawa T;Sugihara A, Noda H, Fukuda H, Tominaga Y .[J].Journal of the American Oil Chemists'Society,1999,76(07):789. |
| [12] | Kaieda M;Samukawa T;Kondo A;Fukuda H .[J].Journal of Bioscience and Bioengineering,2001,91(01):12. |
| [13] | 刘森林,宗敏华,余少文,张小云,邢苗.有机溶剂中（R）-醇腈酶催化不对称合成（R）-苯乙醇腈[J].催化学报,2003(01):7-10. |
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