以壳聚糖微球(CS-MS)为载体,戊二醛(GA)为交联剂,制备固定化乙酰胆碱酯酶(AChE).通过测定各种条件对酶活回收率影响,确定适宜的固定条件为:将1 g CS-MS用5 mL体积分数0.5%的GA活化0.25 h,然后加到含0.75 UAChE、pH 7.0～7.5的PBS中振荡反应24 h.所得到的酶活回收率可达81.94%,优于同类研究水平.红外光谱分析证实,AChE通过共价键和氢键结合到载体上.扫描电镜观察表明,经活化后CS-MS呈蜂窝状且表面平整光滑,利于酶的固定.因此,CS-MS是一种很有潜力的固定AChE栽体.
参考文献
| [1] | Wessa T;Ache HJ;Rapp M .New immobilization method for SAW-biosensors: Covalent attachment of antibodies via CNBr[J].Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices,1999(1):93-98. |
| [2] | Lee HS.;Kim YA.;Cho YA.;Lee YT. .Oxidation of organophosphorus pesticides for the sensitive detection by a cholinesterase-based biosensor[J].Chemosphere,2002(4):571-576. |
| [3] | Denkbas EB.;Odabasi M. .Chitosan microspheres and sponges: Preparation and characterization[J].Journal of Applied Polymer Science,2000(11):1637-1643. |
| [4] | Tien-Chieh Hung;R.Giridhar;Shao-Hua Chiou;Wen-Teng Wu .Binary immobilization of Candida rugosa lipase on chitosan[J].Journal of molecular catalysis, B. Enzymatic,2003(1/2):69-78. |
| [5] | Chaodan Prabhu;Snehal Wanjari;Sonia Gawande .Immobilization of carbonic anhydrase enriched microorganism on biopolymer based materials[J].Journal of molecular catalysis, B. Enzymatic,2009(1/2):13-21. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%