为了改善钛表面的血液相容?用层层自组装技术将抗凝药物肝素和细胞外基质成分之一的胶原层层交替吸附到钛表面,形成多层薄膜,使具有良好的抗凝血功能.钛通过氢氧化钠处理表面带负电,在聚赖氨酸溶液中会吸附一层带正电的聚赖氨酸,然后将其交替浸泡于带负电的肝素和带正电的胶原中形成自组装薄膜.通过傅立叶红外漫反射(FTIR)检测材料表面基团的变化,通过测水接触角的方法跟踪组装过程,通过扫描电子显微镜(SEM)观察材料表面形貌的变化.血小板黏附实验显示钛改性后表面血小板黏附量明显减少,且反映血小板激活程度的P-selectin的表达量结果也显示,钛改性后表面血小板激活程度显著降低,活化部分凝血活酶时间(APTT)和外源性凝血系统的凝血酶原时间(PT)的实验显示钛改性后表面凝血时间延长,血液相容性得到改善.研究说明这种方法对于心血管材料钛的改性有重要价值.
参考文献
| [1] | Tsai CC;Chang Y;Sung HW;Hsu JC;Chen CN .Effects of heparin immobilization on the surface characteristics of a biological tissue fixed with a naturally occurring crosslinking agent (genipin): an in vitro study.[J].Biomaterials,2001(6):523-533. |
| [2] | Decher G .[J].Science,1997,277:1232-1237. |
| [3] | Decher G;Hong J D;Schmitt J .[J].Thin Solid Films,1992,210-211:831-835. |
| [4] | Lvov Y.;Ichinose I.;Kunitake T.;Ariga K. .ASSEMBLY OF MULTICOMPONENT PROTEIN FILMS BY MEANS OF ELECTROSTATIC LAYER-BY-LAYER ADSORPTION[J].Journal of the American Chemical Society,1995(22):6117-6123. |
| [5] | Shi L X;Sun J Q;Liu J Q et al.[J].Chemstry Letters,2002,32:1168-1169. |
| [6] | Trau D.;Yang WJ.;Seydack M.;Caruso F.;Yu NT.;Renneberg R. .Nanoencapsulated microcrystalline particles for superamplified biochemical assays[J].Analytical chemistry,2002(21):5480-5486. |
| [7] | Chung A J;Rubner M F .[J].Langmuir,2002,18:11761. |
| [8] | Salditt Tim;Schubert U S .[J].Reviews in Molecular Biotechnology,2002,90:55-70. |
| [9] | Cai K;Rechtenbach A;Hao J et al.[J].Biomaterials,2005,26:5960-5971. |
| [10] | Grant G G S;Koktysh D S;Yun B G et al.[J].Biomedical Microdevices,2001,3:301-306. |
| [11] | Hong J;Andersson J;Ekdahl KN;Elgue G;Axen N;Larsson R;Nilsson B .Titanium is a highly thrombogenic biomaterial: possible implications for osteogenesis.[J].Thrombosis and Haemostasis: Journal of the International Society on Thrombosis and Haemostasis,1999(1):58-64. |
| [12] | Picart C;Ladam G;Senger B et al.[J].Journal of Chemical Physics,2001,115:1086-1094. |
| [13] | Tamada Y.;Ikada Y.;Kulik EA. .SIMPLE METHOD FOR PLATELET COUNTING[J].Biomaterials,1995(3):259-261. |
| [14] | Wu Y D;Zhou Z R;Meyerhoff M E .[J].Journal of Biomedical Materials Research Part A,2007,1A(04):956-963. |
| [15] | Ratner B D .[J].Journal of Biomaterials Science:Polymer Edition,2000(11):1107-1119. |
| [16] | Xue L;Greisler HP .Biomaterials in the development and future of vascular grafts.[J].Journal of vascular surgery,2003(2):472-480. |
| [17] | Matsuda T.Photochemically-driven Surface Process Technologies for Biocompatible Design of Fabricated Cardiovascular Devices[A].New Orleans,1997 |
| [18] | Salditt Tim;Schubea Ulrich S .[J].Reviews in Molecular Biotechnology,2002,90(01):55-70. |
| [19] | Nishiguchi S;Nakamura T;Kobayashi M;Kim HM;Miyaji F;Kokubo T .The effect of heat treatment on bone-bonding ability of alkali-treated titanium.[J].Biomaterials,1999(5):491-500. |
| [20] | Plepis A M G;Das-Gupta D K;Goissis G .[J].International Symposium on Electrets,1999,10:233-236. |
| [21] | Baier R E;De Palma V A;Coupil D W et al.[J].Journal of Biomedical Materials Research,1985,19:1157-1167. |
| [22] | Yang P;Huang N;Leng YX;Chen JY;Sun H;Wang J;Wan GJ .Inhibition of adherent platelet activation produced by Ti-O thin film fabricated by PIII[J].Surface & Coatings Technology,2004(1/2):265-269. |
| [23] | Groth T.;Herrmann K.;Seifert B.;Campbell EJ. .APPLICATION OF ENZYME IMMUNOASSAYS FOR TESTING HAEMOCOMPATIBILITY OF BIOMEDICAL POLYMERS[J].Biomaterials,1995(13):1009-1015. |
| [24] | Christian D.Low Molecular Weight Heparin Clinical Practice[M].New York:Dekker,1992:126. |
| [25] | Olson S T;Bjork I .[J].Journal of Biological Chemistry,1991,266(10):6353-6364. |
| [26] | Maaroufi R M;Jozefowicz M;Tapon-Bretaudiere J et al.[J].BIOMATERIALS,1997,18(03):203-211. |
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