通过表面改性引入活性生物分子可以用来增强材料表面的生物相容性。采用静电自组装技术将明胶和聚乙烯亚胺引入羟基磷灰石/聚酰胺6（HA/PA6）材料表面,最终获得大分子修饰的复合材料。利用水接触角测量（WCA）、X射线光电子能谱（XPS）和原子力显微镜（AFM）测试手段对表面改性前后HA/PA6膜的表面化学、亲水性和表面形貌进行表征。并研究了其对细胞活性的影响。结果表明,改性后膜表面的亲水性增强,粗糙度增大。体外细胞实验表明,明胶涂层后的HA/PA6表面细胞更利粘附、铺展和生长。该固定方法模拟细胞外基质组成制备出的生物活性膜能进一步满足生物医学工程需求。
Surface engineering of hydroxyapatite/polyamide 6（HA/PA6） membrane via electrostatic self-assembly of extracellular matrix-like molecules was used to improve its surface biocompatibility.The polyelectrolyte-mediated electro-static adsorption of gelatin（gel） and poly（ethylene imine）（PEI） was employed to surface modify HA/PA6 membrane.The surface chemistry,wettability and surface topography of the coated films with different number of deposited layers were investigated by using WCA measurement,XPS and AFM,respectively.The results indicated that the hydrophilicity of modified membranes was improved,and the modified surface exhibited an increase in roughness.Cell culturing on the gelatin coating HA/PA6 membrane surfaces showed improved cell spreading and growth.
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