采用表面引发原子转移自由基聚合(ATRP)的方法制备了聚(N-异丙基丙烯酰胺)(PNIPAAm)改性表面,通过改变聚合时间进而调控表面接枝层厚度.水接触角和蛋白质吸附测试发现PNIPAAm改性表面的浸润性和对蛋白质吸附都具有一定的温度响应性,且随着PNIPAAm接枝层厚度的增加,该温度响应性有一定程度的增强.同时,纤维蛋白原和溶菌酶的吸附测试表明PNIPAAm改性表面对尺寸不同的蛋白质具有不同的温度响应性.
参考文献
| [1] | Cole MA;Voelcker NH;Thissen H;Griesser HJ .Stimuli-responsive interfaces and systems for the control of protein-surface and cell-surface interactions.[J].Biomaterials,2009(9):1827-1850. |
| [2] | Paula M Mendes .Stimuli-responsive surfaces for bio-applications[J].Chemical Society Reviews,2008(11):2512-2529. |
| [3] | Noriaki Matsuda;Tatsuya Shimizu;Masayuki Yamato;Teruo Okano .Tissue Engineering Based on Cell Sheet Technology[J].Advanced Materials,2007(20):3089-3099. |
| [4] | da-Silva RM;Mano JF;Reis RL .Smart thermoresponsive coatings and surfaces for tissue engineering: switching cell-material boundaries.[J].Trends in biotechnology,2007(12):577-583. |
| [5] | Hong Chen;Lin Yuan;Wei Song .Biocompatible polymer materials: Role of protein-surface interactions[J].Progress in Polymer Science,2008(11):1059-1087. |
| [6] | Yamanaka H.;Yoshizako K.;Akiyama Y.;Sota H.;Hasegawa Y.;Shinohara Y. Kikuchi A.;Okano T. .Affinity chromatography with collapsibly tethered ligands[J].Analytical Chemistry,2003(7):1658-1663. |
| [7] | Hongwei Ma;Dejin Li;Xia Sheng;Bin Zhao;Ashutosh Chilkoti .Protein-Resistant Polymer Coatings on Silicon Oxide by Surface-Initiated Atom Transfer Radical Polymerization[J].Langmuir: The ACS Journal of Surfaces and Colloids,2006(8):3751-3756. |
| [8] | Wu, ZQ;Chen, H;Liu, XL;Zhang, YX;Li, D;Huang, H .Protein Adsorption on Poly(N-vinylpyrrolidone)-Modified Silicon Surfaces Prepared by Surface-Initiated Atom Transfer Radical Polymerization[J].Langmuir,2009(5):2900-2906. |
| [9] | Feng W;Zhu SP;Ishihara K;Brash JL .Adsorption of fibrinogen and lysozyme on silicon grafted with poly(2-methacryloyloxyethyl phosphorylcholine) via surface-initiated atom transfer radical polymerization[J].Langmuir: The ACS Journal of Surfaces and Colloids,2005(13):5980-5987. |
| [10] | Yu Q;Zhang Y;Chen H et al.Protein adsorption on poly (N-isopropylacrylamide)-modified silicon surfaces:Effects of grafted layer thickness and protein size[J].Colloids and Surfaces B:Biointerfaces,2010,76:468. |
| [11] | Taolei Sun;Guojie Wang;Lin Feng;Biqian Liu;Yongmei Ma;Lei Jiang;Daoben Zhu .Reversible Switching between Superhydrophilicity and Superhydrophobicity[J].Angewandte Chemie,2004(3):357-360. |
| [12] | H.Yim;M.S.Kent;S.Mendez;G.P.Lopez;S.Satija;Y.Seo .Effects of Grafting Density and Molecular Weight on the Temperature-Dependent Conformational Change of Poly(N-isopropylacrylamide) Grafted Chains in Water[J].Macromolecules,2006(9):3420-3426. |
| [13] | Prime K L;Whitesides G M .Adsorption of proteins onto surfaces containing end-attached oligo (ethylene oxide):A model system using self-assembled monolayers[J].Journal of the American Chemical Society,1993,115(23):10714. |
| [14] | Halperin A .Polymer brushes that resist adsorption of model proteins:design parameters[J].Langmuir,1999,15(07):2525. |
| [15] | Norde W;Gage D .Interaction of bovine serum albumin and human blood plasma with peo-tethered surfaces:Influence of peo chain length,grafting density,and temperature[J].Langmuir,2004,20:4162. |
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