{"currentpage":1,"firstResult":0,"maxresult":10,"pagecode":5,"pageindex":{"endPagecode":3,"startPagecode":1},"records":[{"abstractinfo":"采用液相色谱-质谱联用方法分析了中药<span style=\"color:red\">大黄</span>经过SD大鼠肝匀浆体外代谢前后的指纹谱中色谱峰面积、保留值的差异.指出5种游离型蒽醌化合物在SD大鼠肝匀浆体外代谢体系中只有<span style=\"color:red\">大黄</span>酚发生代谢反应转化为芦荟<span style=\"color:red\">大黄</span>素.考察了体外代谢条件下,肝匀浆浓度与代谢时间对<span style=\"color:red\">大黄</span>酚转化及其代谢产物的影响.SD大鼠体外抗肿瘤试验表明,<span style=\"color:red\">大黄</span>代谢物对于人宫颈癌(HeLa)细胞的抑制活性略高于其提取物.通过比较芦荟<span style=\"color:red\">大黄</span>素、<span style=\"color:red\">大黄</span>酸、<span style=\"color:red\">大黄</span>素、<span style=\"color:red\">大黄</span>酚、<span style=\"color:red\">大黄</span>素甲醚的活性,并结合<span style=\"color:red\">大黄</span>酚的体外代谢反应的考察,解释了<span style=\"color:red\">大黄</span>代谢物对肿瘤细胞活性的抑制率的提高是由<span style=\"color:red\">大黄</span>酚的代谢产物芦荟<span style=\"color:red\">大黄</span>素浓度的增加引起的.","authors":[{"authorName":"孔亮","id":"5fe680d3-19e9-4ab1-a186-49917cea2434","originalAuthorName":"孔亮"},{"authorName":"包永明","id":"dd8ae983-2aa2-4c4d-8ac3-448e3d473555","originalAuthorName":"包永明"},{"authorName":"于志远","id":"536ac043-1785-41cc-a527-94f0a87ce9c0","originalAuthorName":"于志远"},{"authorName":"李伟","id":"8c079e6b-8e84-4dae-8bce-c9b162801556","originalAuthorName":"李伟"},{"authorName":"陈学国","id":"68226e61-2974-4263-ac5f-79d3a53f5bee","originalAuthorName":"陈学国"},{"authorName":"胡良海","id":"36a944f7-3900-44b3-887c-db7a0169bc46","originalAuthorName":"胡良海"},{"authorName":"邹汉法","id":"4c9560b8-b73d-4ce4-9ed7-04b131d3d512","originalAuthorName":"邹汉法"}],"doi":"10.3321/j.issn:1000-8713.2005.05.007","fpage":"470","id":"ca3a5fcd-d3da-4814-a299-1aecd1ac6cca","issue":"5","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"0e3bd7bc-6988-497c-bed3-8b4d27353379","keyword":"高效液相色谱-质谱","originalKeyword":"高效液相色谱-质谱"},{"id":"71369bd4-57d0-4b58-89b6-2872e08e2c5c","keyword":"<span style=\"color:red\">大黄</span>","originalKeyword":"大黄"},{"id":"fb05cbc3-af12-4b8a-952e-24ed541fdbc2","keyword":"色谱指纹谱","originalKeyword":"色谱指纹谱"},{"id":"02f461e2-9770-4f93-b0e0-1967cdd2acc4","keyword":"体外代谢","originalKeyword":"体外代谢"},{"id":"a8b1e410-88ae-46f0-bc24-b2e32b4680bc","keyword":"中药","originalKeyword":"中药"},{"id":"19198397-6cd5-4c64-8bc7-bde37883d020","keyword":"SD大鼠","originalKeyword":"SD大鼠"},{"id":"b1909143-44b7-4efa-8a85-8cfe57ce6637","keyword":"肝匀浆","originalKeyword":"肝匀浆"}],"language":"zh","publisherId":"sp200505007","title":"色谱指纹谱用于中药<span style=\"color:red\">大黄</span>抗肿瘤活性成分的筛选","volume":"23","year":"2005"},{"abstractinfo":"以1,8-二羟基蒽醌为对照,利用热重-差热分析仪对从<span style=\"color:red\">大黄</span>中分离纯化的游离羟基蒽醌(<span style=\"color:red\">大黄</span>酸、<span style=\"color:red\">大黄</span>素、芦荟<span style=\"color:red\">大黄</span>素、<span style=\"color:red\">大黄</span>素甲醚和<span style=\"color:red\">大黄</span>酚)进行了测定,得到这些样品的热分析图谱. 通过对其进行特征分析,结果表明各样品的TG曲线形状基本一致,但由于各单体中的1,8-二羟基蒽醌上所带基团不同,有不同的失重情况;所有苷元的DTA曲线都有2个明显的特征峰,但其峰形、峰位置和大小有明显的区别,因此可以用热重-差热分析法来鉴定从<span style=\"color:red\">大黄</span>药材分离纯化的游离羟基蒽醌.","authors":[{"authorName":"张黎明","id":"316d5f73-a4e3-49f3-adbf-77c408989f4e","originalAuthorName":"张黎明"},{"authorName":"李霞","id":"3ec02564-6fd0-46fa-b674-322e1368d487","originalAuthorName":"李霞"},{"authorName":"曹井国","id":"486ed334-761f-4af6-a67c-dbe5287ace75","originalAuthorName":"曹井国"}],"doi":"10.3969/j.issn.1000-0518.2006.09.027","fpage":"1058","id":"95c6abf1-6094-4afe-a178-99b6851e2932","issue":"9","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"2a5fb479-d045-44fb-ac79-47223becd3b1","keyword":"<span style=\"color:red\">大黄</span>","originalKeyword":"大黄"},{"id":"99853cc9-cb99-41d7-8ec8-a5c9975bc128","keyword":"游离羟基蒽醌","originalKeyword":"游离羟基蒽醌"},{"id":"8cbd2b89-5e2f-4a74-9b47-fe8e5a9e5d9b","keyword":"热重法(TG)","originalKeyword":"热重法(TG)"},{"id":"04bfa717-09b1-43d1-9495-0510afc09240","keyword":"差热分析(DTA)","originalKeyword":"差热分析(DTA)"}],"language":"zh","publisherId":"yyhx200609027","title":"<span style=\"color:red\">大黄</span>游离羟基蒽醌的TG-DTA分析","volume":"23","year":"2006"},{"abstractinfo":"研究了<span style=\"color:red\">大黄</span>中鞣质类成分的提取、分离与分析方法.优化了<span style=\"color:red\">大黄</span>原药材中鞣质类物质的提取方法;建立了<span style=\"color:red\">大黄</span>鞣质类成分的梯度洗脱反相高效液相色谱(HPLC)分析方法,使鞣质类成分得到了良好的分离;采用液相色谱-质谱(LC-MS)对<span style=\"color:red\">大黄</span>中主要的鞣质类化合物进行了结构分析,并总结了一部分鞣质类化合物在高效液相色谱-电喷雾质谱(HPLC-ESI-MS)谱图上的裂解规律.","authors":[{"authorName":"丁明玉","id":"2dbb513b-5646-426e-bd97-a7619f2d7ce3","originalAuthorName":"丁明玉"},{"authorName":"倪为为","id":"61e0c20f-ca1c-454a-8da9-a75cbf073171","originalAuthorName":"倪为为"}],"doi":"10.3321/j.issn:1000-8713.2004.06.008","fpage":"605","id":"aaaa20b0-5709-4727-a94a-97800744a8c7","issue":"6","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"89d6ef98-edb9-4746-910c-8cb67c68586c","keyword":"高效液相色谱法","originalKeyword":"高效液相色谱法"},{"id":"4a614bc0-42ab-4f51-8cc7-ee6b7370407f","keyword":"质谱","originalKeyword":"质谱"},{"id":"172c3c2d-09a1-4394-8f09-011c4fc6c3ab","keyword":"鞣质","originalKeyword":"鞣质"},{"id":"c8cd2945-06b5-4e52-9103-e42c244d6533","keyword":"<span style=\"color:red\">大黄</span>","originalKeyword":"大黄"}],"language":"zh","publisherId":"sp200406008","title":"<span style=\"color:red\">大黄</span>中鞣质成分的分离与液相色谱/质谱联用分析","volume":"22","year":"2004"},{"abstractinfo":"探讨了迁移时间归一化法改善中药毛细管电泳分析迁移时间重现性的原理,并将其应用于实际样品的分析.迁移时间归一化法认为,在相同的操作电压、缓冲液组成和温度条件下,多次电泳实验中迁移时间产生差别的主要原因是多次电泳实验中电渗流产生了差异.迁移时间归一化法就是通过选择电泳谱图中的一个或两个峰作为标记峰,将各次电泳实验的迁移时间都归一到第一次电泳实验中的迁移时间.比较多次电泳实验中迁移时间(t)的相对标准偏差(RSD)、经单峰归一化处理的迁移时间(t′)的RSD、经双峰归一化处理的迁移时间(t″)的RSD、迁移时间比(t/tistd,istd代表所选择的标记物)的RSD,发现RSD(t″) <RSD(t′) <RSD(t/tistd)和RSD(t).考察了中药<span style=\"color:red\">大黄</span>5次胶束电动色谱分离中迁移时间的重现性,其RSD(t″)在0.07% ～0.58%之间.还考察了不同基质的<span style=\"color:red\">大黄</span>样品在毛细管区带电泳模式下迁移时间的重现性,发现所选择的7个明显的电泳峰的RSD(t″)在0.31% ～0.57%之间.结果表明,迁移时间归一化法能有效地提高中药<span style=\"color:red\">大黄</span>样品迁移时间的重现性.该方法有望在中药毛细管电泳表征方面发挥更大的作用.","authors":[{"authorName":"张红医","id":"9b9ff52a-328b-49b7-b765-cf63b1480947","originalAuthorName":"张红医"},{"authorName":"盖丽娟","id":"d19d6cbe-f5f9-4996-b15c-a2ed5aaf4745","originalAuthorName":"盖丽娟"},{"authorName":"陈辉","id":"8cb3f597-c3f5-4c56-b177-62f2313e6346","originalAuthorName":"陈辉"},{"authorName":"景聪","id":"6cf05377-51ef-40f0-a65e-a4d5f12aaf00","originalAuthorName":"景聪"},{"authorName":"石志红","id":"bfa532dc-92db-41bf-bd7e-c3be5ba703cf","originalAuthorName":"石志红"}],"doi":"10.3321/j.issn:1000-8713.2009.04.022","fpage":"489","id":"dfcca1d2-1cb4-4d9b-8dab-d3ed4494f3d9","issue":"4","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"36cf5630-9e12-469a-a0d0-07d42c201eb2","keyword":"毛细管电泳","originalKeyword":"毛细管电泳"},{"id":"830c2d14-f8cf-4057-8d72-d4e7ff7c34b0","keyword":"迁移时间归一化法","originalKeyword":"迁移时间归一化法"},{"id":"cc353bf1-b4a8-427e-937f-31f4dc534113","keyword":"重现性","originalKeyword":"重现性"},{"id":"78c7063f-b311-4e4d-a519-dde335f324e2","keyword":"<span style=\"color:red\">大黄</span>","originalKeyword":"大黄"},{"id":"1c867255-4f75-4fdb-8250-f3da57b7d64a","keyword":"中药","originalKeyword":"中药"}],"language":"zh","publisherId":"sp200904022","title":"迁移时间归一化法改善毛细管电泳表征中药<span style=\"color:red\">大黄</span>的重现性","volume":"27","year":"2009"},{"abstractinfo":"以中国药典收载的六味安消胶囊中<span style=\"color:red\">大黄</span>素与<span style=\"color:red\">大黄</span>酚的含量测定方法为典型案例,揭示了色谱柱是影响HPLC质量控制方法测定结果准确性的关键风险因素.参加能力验证的155家实验室均采用方法规定的C18柱,但由于不同品牌C18柱间的选择性差异,有20家实验室的分析结果中<span style=\"color:red\">大黄</span>素峰与样品水解产物的色谱峰共流出,导致测定结果明显偏离正常值.统计结果表明,A型(早期开发、硅羟基残留较多)和E型(内嵌极性基团或以极性基团封尾)C18柱更适于六味安消胶囊中<span style=\"color:red\">大黄</span>素与<span style=\"color:red\">大黄</span>酚的含量测定,这可能是因为<span style=\"color:red\">大黄</span>素和<span style=\"color:red\">大黄</span>酚结构中含有多个酚羟基,能与A型柱的残留硅羟基或E型柱内嵌/封尾的极性基团相互作用,从而增强其保留行为有关.建议相关实验室在制订HPLC质量控制方法时应特别注重色谱柱的耐用性考察,并尝试采用国外色谱柱分类数据库科学地指导色谱柱的选择,尽量采用难分离物质对作为系统适用性指标,完善质量标准,减少由于色谱柱选择不适宜导致测定结果不准确的风险.","authors":[{"authorName":"王明娟","id":"63a3bbcb-3b2b-4f57-8691-48333dfd4bcb","originalAuthorName":"王明娟"},{"authorName":"刘雅丹","id":"5dbb252e-23af-42a1-889b-9df02d58f740","originalAuthorName":"刘雅丹"},{"authorName":"胡晓茹","id":"5d43a6ed-058a-4cbd-9f19-f1b671ffe626","originalAuthorName":"胡晓茹"},{"authorName":"何风艳","id":"1547ed47-bc15-4b1a-a4c0-6eae6486972e","originalAuthorName":"何风艳"},{"authorName":"项新华","id":"19aa976a-44d8-43bf-aea7-e1d3c12d3cdc","originalAuthorName":"项新华"},{"authorName":"戴忠","id":"d769e1b1-1c60-429b-b7ae-21ff11e4ac2d","originalAuthorName":"戴忠"},{"authorName":"于健东","id":"6242c0a5-a066-4938-950e-39eadc58136c","originalAuthorName":"于健东"},{"authorName":"马双成","id":"32956a33-3dcf-445b-9a37-d510f2985d47","originalAuthorName":"马双成"}],"doi":"10.3724/SP.J.1123.2016.08008","fpage":"1077","id":"b5fbbe11-1b5a-425f-bf30-ea076e928c8d","issue":"11","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"29cbed79-d271-4b5f-961f-707b2cdf3037","keyword":"高效液相色谱","originalKeyword":"高效液相色谱"},{"id":"c866d3a1-9083-429b-beea-d7aac75b2f3b","keyword":"色谱柱选择","originalKeyword":"色谱柱选择"},{"id":"7e665ffc-b2b8-4e6f-b7bf-521f0d913ae8","keyword":"关键风险因素","originalKeyword":"关键风险因素"},{"id":"400f823f-46d4-4989-9cc6-0b181e93db6f","keyword":"<span style=\"color:red\">大黄</span>素","originalKeyword":"大黄素"},{"id":"53004221-9418-45cb-aa4a-2ce6ebe5b13a","keyword":"<span style=\"color:red\">大黄</span>酚","originalKeyword":"大黄酚"},{"id":"ec4f5295-e73e-4024-b91d-d28a73a01d73","keyword":"六味安消胶囊","originalKeyword":"六味安消胶囊"}],"language":"zh","publisherId":"sp201611009","title":"色谱柱选择对六味安消胶囊中<span style=\"color:red\">大黄</span>素和<span style=\"color:red\">大黄</span>酚含量测定的影响","volume":"34","year":"2016"},{"abstractinfo":"以NF270纳滤复合膜对几种不同分子量的天然药物(如<span style=\"color:red\">大黄</span>素、咖啡因)进行了纳滤行为研究,并考察了操作压力、浓度、pH、离子浓度等因素对纳滤膜截留行为的影响.结果表明,NF270纳滤复合膜截留分子量在300左右(例如对相对分子质量为270.23的<span style=\"color:red\">大黄</span>素几乎100%截留,对相对分子质量较小的咖啡因截留率小于60%);随着pH的增大,纳滤膜对<span style=\"color:red\">大黄</span>素的截留率稍有下降,对其他三种药物的截留率略有升高;改变溶液的离子浓度,纳滤膜的截留率和通量均下降.选择适宜的操作条件可以实现天然药物的提取、分离和浓缩.","authors":[{"authorName":"王刚","id":"d909f8bc-71a5-4e8b-aba5-ef87c8d4eae1","originalAuthorName":"王刚"},{"authorName":"张宇峰","id":"f1a07938-9585-4f25-b64a-a5db0c8f109b","originalAuthorName":"张宇峰"},{"authorName":"郭豪","id":"2c1e1537-f195-4ba5-a781-09f0df19b885","originalAuthorName":"郭豪"},{"authorName":"王翔","id":"1c1d8567-a5e0-4ed5-9847-b2a33962fb8c","originalAuthorName":"王翔"}],"doi":"10.3969/j.issn.1007-8924.2009.03.005","fpage":"23","id":"27df4f2a-70f4-4457-ac7b-9899e8ef80cd","issue":"3","journal":{"abbrevTitle":"MKXYJS","coverImgSrc":"journal/img/cover/MKXYJS.jpg","id":"54","issnPpub":"1007-8924","publisherId":"MKXYJS","title":"膜科学与技术   "},"keywords":[{"id":"82318227-1d96-4711-91c6-ab65e0ad332d","keyword":"纳滤","originalKeyword":"纳滤"},{"id":"5d660b74-2863-44f3-ad6d-f5cdd1fa93a4","keyword":"天然药物","originalKeyword":"天然药物"},{"id":"38e4ca61-0c78-4acb-90bc-bc05d7a615d2","keyword":"截留率","originalKeyword":"截留率"},{"id":"e3161516-560e-4af5-9a3b-75a815a5ed4a","keyword":"通量","originalKeyword":"通量"},{"id":"d6dc1ff0-73dd-40e1-a64c-62c0230b4b50","keyword":"离子浓度","originalKeyword":"离子浓度"}],"language":"zh","publisherId":"mkxyjs200903005","title":"<span style=\"color:red\">大黄</span>素等天然药物的纳滤行为","volume":"29","year":"2009"},{"abstractinfo":"采用反相高效液相色谱法考察注射用<span style=\"color:red\">大黄</span>粉针剂在不同梯度洗脱条件下的分离情况,优化的注射用<span style=\"color:red\">大黄</span>粉针剂高效液相色谱指纹图谱的分离条件是:色谱柱为Shim-pack CLC-ODS柱(6.0 mm i.d.×150 mm,5 μm);流动相为甲醇和0.1%(体积分数)磷酸溶液体系;检测波长254 nm;柱温40 ℃.在该色谱条件下,注射用<span style=\"color:red\">大黄</span>粉针剂各组分达到较佳分离,方法的稳定性、精密度、重现性好,为建立其指纹图谱奠定了基础.","authors":[{"authorName":"徐雄良","id":"6c519b19-f870-4d56-a107-1189803c57dc","originalAuthorName":"徐雄良"},{"authorName":"张志荣","id":"844a134e-f3e3-4391-a320-0aa39598691f","originalAuthorName":"张志荣"},{"authorName":"黄园","id":"f67f3499-7d0b-44dd-9c98-12ae43a14b3b","originalAuthorName":"黄园"}],"doi":"10.3321/j.issn:1000-8713.2003.06.016","fpage":"596","id":"3e289f54-17c5-4d1a-84e7-7dd6d2d4b486","issue":"6","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"11c8a5e9-10bf-44e5-a430-669141acddc3","keyword":"反相高效液相色谱法","originalKeyword":"反相高效液相色谱法"},{"id":"bfdf92e5-2690-4391-950c-18e4fc5ca854","keyword":"指纹图谱","originalKeyword":"指纹图谱"},{"id":"144f0df8-dbd8-4396-a7ab-a5618f04a0e1","keyword":"注射用<span style=\"color:red\">大黄</span>粉针剂","originalKeyword":"注射用大黄粉针剂"}],"language":"zh","publisherId":"sp200306016","title":"注射用<span style=\"color:red\">大黄</span>粉针剂的高效液相色谱分析","volume":"21","year":"2003"},{"abstractinfo":"药物洗脱支架具有良好的抗增生性能,对血管支架内再狭窄起到很好的抑制作用,但其抗凝血性能仍有待改善.<span style=\"color:red\">大黄</span>素(emodin)是一类具有抗增生以及抑制血小板黏附聚集等特性的中药提取物,有望成为药物洗脱支架的选用药物.本文以可降解高分子材料聚乳酸(PLA)为载体制备了浓度为3%(质量分数)、5%(质量分数)的<span style=\"color:red\">大黄</span>素复合薄膜.采用傅立叶变换红外光谱研究了复合薄膜的组成成分,用体外血小板粘附实验和部分凝血活酶时间(APTT)表征复合薄膜的体外抗凝血性能.结果显示<span style=\"color:red\">大黄</span>素与PLA的特征峰在复合薄膜的红外图谱中均有出现,复合薄膜的血小板粘附和聚集数量减少且APTT长于纯PLA薄膜,这都表明<span style=\"color:red\">大黄</span>素/聚乳酸复合薄膜的抗凝血性能优于纯PLA薄膜.","authors":[{"authorName":"唐家驹","id":"a4c1b428-3c82-4fb2-ad57-a979901e2ea9","originalAuthorName":"唐家驹"},{"authorName":"潘长江","id":"d272b734-e117-4bb4-b84f-bfd5810a0d4c","originalAuthorName":"潘长江"},{"authorName":"洪玮","id":"1f4a486f-9d6b-4f89-95c6-e5bf52f71e82","originalAuthorName":"洪玮"},{"authorName":"王进","id":"dd46bcb5-1080-4751-b6bf-e6dcc96e2266","originalAuthorName":"王进"},{"authorName":"黄楠","id":"dab202d4-1d4e-4892-bff0-1d007498cb41","originalAuthorName":"黄楠"}],"doi":"","fpage":"1312","id":"5169e436-c00e-4701-a4c2-42b4175abae7","issue":"8","journal":{"abbrevTitle":"GNCL","coverImgSrc":"journal/img/cover/GNCL.jpg","id":"33","issnPpub":"1001-9731","publisherId":"GNCL","title":"功能材料"},"keywords":[{"id":"7dc21e0e-9635-4066-9170-f3492c5c76c4","keyword":"<span style=\"color:red\">大黄</span>素","originalKeyword":"大黄素"},{"id":"87c6ad52-88ff-4b3d-92e2-8fd0f8ca5f4e","keyword":"PLA","originalKeyword":"PLA"},{"id":"4add0923-1f78-4bb9-abb2-552c543fa235","keyword":"抗凝血","originalKeyword":"抗凝血"},{"id":"1ed7051d-3971-4ac6-b669-cd3f98ef091f","keyword":"复合薄膜","originalKeyword":"复合薄膜"}],"language":"zh","publisherId":"gncl200608039","title":"<span style=\"color:red\">大黄</span>素/聚乳酸物复合薄膜的制备及抗凝血研究","volume":"37","year":"2006"},{"abstractinfo":"采用中间体法，先将<span style=\"color:red\">大黄</span>素配体与γ-[（2，3）-环氧丙氧]丙基三甲氧基硅烷（KH-560）偶联剂反应制备含配体的硅氧烷试剂，然后再与硅胶键合，最终制得<span style=\"color:red\">大黄</span>素键合硅胶液相色谱固定相（以下简称 ESP）。通过红外光谱、元素分析和热重分析表征固定相的结构。以萘作为溶质探针，甲醇-水（60：40，v / v）为流动相，流速为0.8 mL / min，测得 ESP 柱的柱效。采用传统的反相 C18和苯基柱作参比，将 ESP 应用于系列中性、碱性和酸性芳香族化合物以及实际样品风油精的分离分析，并探讨相关的色谱分离机理。结果表明，配体<span style=\"color:red\">大黄</span>素被成功地键合到球形硅胶表面，测得配体键合量为0.23 mmol / g，ESP 柱理论塔板数约为19874 N / m。ESP 的偶联剂链和蒽醌环提供了疏水性的结构基础，<span style=\"color:red\">大黄</span>素配体还能为溶质提供π-π或 p-π、电荷转移、氢键、偶极-偶极等作用点。多位点的协同作用使得 ESP 柱具有独特和优秀的色谱分离选择性，并且无需调节 pH 值，采用简单而廉价的甲醇-水流动相就能实现胺类、酚类等极性样品的基线分离，实验条件简单、方便。","authors":[{"authorName":"许丽丽","id":"31f651ff-f35d-4616-a2cd-58ee6778f55c","originalAuthorName":"许丽丽"},{"authorName":"任乃林","id":"7e81b845-72c7-4f2a-b1fb-199f091471b4","originalAuthorName":"任乃林"},{"authorName":"林泽玲","id":"5421a0f2-a097-4864-b0d9-771bc4b0ac99","originalAuthorName":"林泽玲"}],"doi":"10.3724/SP.J.1123.2014.04038","fpage":"798","id":"54ff7832-bb25-4342-8e4a-c0eb134c7ca5","issue":"8","journal":{"abbrevTitle":"SP","coverImgSrc":"journal/img/cover/SP.jpg","id":"58","issnPpub":"1000-8713","publisherId":"SP","title":"色谱 "},"keywords":[{"id":"8f204ba5-e55b-485b-b043-dd83237d612d","keyword":"高效液相色谱","originalKeyword":"高效液相色谱"},{"id":"6a4ad731-f822-475c-974f-5b6663b39903","keyword":"键合硅胶固定相","originalKeyword":"键合硅胶固定相"},{"id":"e5ad5452-0fb4-4ab6-a640-1f52eaaa4ea9","keyword":"<span style=\"color:red\">大黄</span>素配体","originalKeyword":"大黄素配体"},{"id":"b4fbf9f7-a51f-4c56-b8aa-c5e8780eb22d","keyword":"色谱柱","originalKeyword":"色谱柱"},{"id":"6b994cd9-6dfb-4c4e-b505-d208072ebbe7","keyword":"制备","originalKeyword":"制备"},{"id":"4d872d08-9083-4f18-b1c8-2985024156d2","keyword":"应用","originalKeyword":"应用"}],"language":"zh","publisherId":"sp201408002","title":"<span style=\"color:red\">大黄</span>素键合硅胶高效液相色谱柱的制备和应用","volume":"","year":"2014"},{"abstractinfo":"在25 ℃条件下,利用毛细管电泳法测得β-环糊精与芦荟<span style=\"color:red\">大黄</span>素在pH=9.7、浓度为0.015 mol/L的硼砂缓冲溶液中的包合反应平衡常数为27.5 L/mol. 由于芦荟<span style=\"color:red\">大黄</span>素具有强疏水性,故其与环糊精的结合力应属于疏水作用力.","authors":[{"authorName":"郭英","id":"89197e5a-79ca-4c56-85b6-7dccad5ae459","originalAuthorName":"郭英"},{"authorName":"崔德生","id":"5482fcf9-3dc9-42f6-b87f-4adf3b9049c8","originalAuthorName":"崔德生"},{"authorName":"汪子明","id":"2c315223-7739-4538-9607-b51eeb7824d2","originalAuthorName":"汪子明"}],"doi":"10.3969/j.issn.1000-0518.2009.04.028","fpage":"492","id":"d28233e1-b6fd-4faf-9d08-caf2844b85c1","issue":"4","journal":{"abbrevTitle":"YYHX","coverImgSrc":"journal/img/cover/YYHX.jpg","id":"73","issnPpub":"1000-0518","publisherId":"YYHX","title":"应用化学"},"keywords":[{"id":"fc8c6693-78e5-44be-b5c4-54c4fd2daedb","keyword":"β-环糊精","originalKeyword":"β-环糊精"},{"id":"1f5d59e5-7a77-4d6d-a181-4679f25e6d18","keyword":"芦荟<span style=\"color:red\">大黄</span>素","originalKeyword":"芦荟大黄素"},{"id":"efb7c4a3-636f-4bc1-a53b-48b79449d83b","keyword":"亲和毛细管电泳","originalKeyword":"亲和毛细管电泳"},{"id":"cfeb1e79-e36c-4efe-88fe-38d81f04637e","keyword":"平衡常数","originalKeyword":"平衡常数"}],"language":"zh","publisherId":"yyhx200904028","title":"毛细管电泳法测定β-环糊精与芦荟<span style=\"color:red\">大黄</span>素包合反应平衡常数","volume":"26","year":"2009"}],"totalpage":3,"totalrecord":28}