为了得到用于角膜修复的材料,将再生丝素蛋白与D-山梨醇或肌醇共混,用流延法制备共混膜。用X射线衍射、红外光谱法对共混膜的结构进行分析,结果显示,共混膜主要以丝素I型结晶结构为主;SEM测试显示,共混膜表面在湿态下形成了纳米孔洞。考察了共混膜的热水溶失率,力学性能及透光率,发现丝素蛋白的热水溶失率均小于2%。D-山梨醇丝素共混膜表现出很好的韧性,而肌醇丝素共混膜表现出一定的脆性。六元醇含量小于40%的共混膜,力学性能均达到了人角膜的性能;透光性良好,与人的角膜相似,有望应用于角膜修复材料。
The transparent water-insoluble Silk fibroin（SF） films were casted from the mixture solution of Silk fibroin and D-sorbitol or inositol.The structure,surfaces,solubility,mechanical properties and light transmittance of the blend films were measured.Fourier transform infrared spectrum（FT-IR）,X-ray diffraction indicate that the films are mainly composed of the crystalline structure of Silk Ⅰ.scanning electron microscope（SEM） shows D-sorbitol/SF blend films are miscible,whereas inositol and SF are phase separated;in the wet state,there are lots of nanoporous in the blend films.The D-sorbitol/SF films which are insoluble have excellent mechanical properties while the inositol/SF films are brittle.the mechanical property of hexitols/SF blend films is consistent with the human cornea in wet state.When the m（D-sorbitol）/m（SF） is from 1/10 to 2/10,the blends films have high light transmittance which are similar to human cornea.In summary,both of the above-mentioned D-sorbitol/SF films provide a great potential to act as repairing materials for cornea.
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