以壳聚糖为原料，首先合成N，N-双长链烷基壳聚糖（DACS），溶于四氢呋喃（THF）中用Nail活化后与1，3-丙烷磺内酯反应，制得N，N-双烷基-3，6-O-磺丙基壳聚糖（SPDACS）两亲性衍生物。用FT-IR、1H-NMR、EA和溶解性实验等对产物进行表征，并研究其在水溶液中的自组装行为。结果表明。SP-DACS能溶于酸性、中性和碱性水溶液中，在水溶液中形成的自组装纳米聚合物胶束为球形结构。随着烷基链长度的增加（8、10、12），临界胶柬浓度（CMC）从0．014mg／mL减小到0．006mg／mL，平均粒径从169．4nm减小到117．0nm。甲苯胺蓝变色实验结果表明SPDACS具有类肝素结构，有望成为血液相容性良好的药物载体。
N, N-dialkyl-3,6-O-sulfopropyl chitosan (SPDACS) was synthesized by the reaction of l, 3-propane sultone and N,N-dialkyl chitosan(DACS) whose hydroxyl groups were activated by Nail using THF as solvent. The products were characterized by FT-IR, 1H NMR, EA and solubility test. The self-assembly behaviors of SPDACS in aqueous solution were studied. The results showed that SPDACS were soluble in acidic, neutral and alkaline aqueous solution. SPDACS self-assembled nanomicelles with the spherical shape was successfully pre- pared in the water. The CMC and the average particle size of SPDACS with heparin structure verified by toluid- ine blue discoloration test decreased from 0. 014mg/mL to 0. 006mg/mL and from 169.4nm to ll7nm with the increase of the carbon chain length from 8 to 12. The results showed that the SPDACS was expected to become a drug carrier with good blood compatibility.
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