玻璃化被认为是低温保存关节软骨的一种有效方法,但需要考虑低温保护剂渗入软骨的速率以及低温保护剂毒性的制约.本文选用二甲亚砜(DMSO)作为低温保护剂,对其在1℃,22℃和37℃时对绵羊关节软骨的渗透特性进行了研究,得到相应的扩散系数为1.9×10-6cm2/s,3.3×10-6cm2/s和5.7×10-6cm2/s,活化能为22.84 kJ/mol.认识低温保护剂对关节软骨的渗透特性有助于建立低温保护剂加入/取出过程的数学模型,从而优化关节软骨的玻璃化保存方案.
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