聚乙烯醇(PVA)是一种常用的酶固定化载体材料,通过一定的工艺手段对其进行改性后能满足不同性质酶的固定化需要.主要介绍了通过物理或化学改性的手段制备聚乙烯醇基酶固定化载体材料,即聚乙烯醇凝胶胶囊、聚乙烯醇冻凝胶、聚乙烯醇网络结构材料、聚乙烯醇基复合材料薄膜、聚乙烯醇电纺丝材料等,并简要评述了这些材料对相应的固定化酶活性和使用稳定性的影响.
参考文献
| [1] | 薛屏,卢冠忠,郭杨龙,王筠松.高分子载体材料对青霉素酰化酶的固定化作用[J].功能高分子学报,2005(02):340-346. |
| [2] | 胡和兵,王牧野,吴勇民,何明中.酶的固定化技术及应用[J].中国酿造,2006(07):4-8. |
| [3] | 拜永孝,李彦锋*,马应霞,周林成,马鹏程.固定化酶技术及其应用[J].化学通报（网络版）,2005(1):1-6. |
| [4] | 洪韫嘉,李谭瑶,陈波,姚守拙.壳聚糖固定化血管紧张素转化酶及其性质[J].高等学校化学学报,2009(02):328-331. |
| [5] | Parascandola P;Branduardi P;de Alteriis E .PVA-gel(Lentikats)as an effective matrix for yeast strain immobilization aimed at heterologous protein production[J].Enzyme and Microbial Technology,2006,38:184. |
| [6] | 潘育松,熊党生,陈晓林.聚乙烯醇水凝胶的制备及性能[J].高分子材料科学与工程,2007(06):228-231. |
| [7] | Wong FL;Abdul-Aziz A .Comparative study of poly(vinyl alcohol)-based support materials for the immobilization of glucose oxidase[J].Journal of Chemical Technology & Biotechnology,2008(1):41-46. |
| [8] | Capan E;Jahnz U;Vorlop K D .Pre-activated Lentikathydrogels for covalent binding of enzymes[J].Practical Aspects of Encapsulation Technologies,2002,241:151. |
| [9] | Groger H;Capan E;Barthuber A et al.Biocatalytic asymmetric hydrocyanation in the presence of-oxynitrilases entrapped in lens-shaped gels[J].Practical Aspects of Encapsulation Technologies,2002,241:87. |
| [10] | Lorena W;lllanes A;Pessela B C C et al.Encapsulation of crosslinked penicillin G acylase aggregates in Lentikats.Evaluation of a novel biocatalyst in organic media[J].Biotechnology and Bioengineering,2004,86(05):558. |
| [11] | Rebros M et al.A simple entrapment of glucoamylase into Lentikats as an efficient catalyst for maltodextrin hydrolysis[J].Enzyme and Microbial Technology,2006,39:800. |
| [12] | Rebros M;Rosenberg M;Mlichova Z et al.Hydrolysis of sucrose by invertase entrapped in polyvinyl alcohol hydrogel capsules[J].Food Chemistry,2007,102:784. |
| [13] | Grosov Z et al.Entrapment of β-galactosidase in polyvinylalcohol hydrogel[J].Biotechnology Letters,2008,30:763. |
| [14] | Fernandes P;Marques P C M;Carvalho F et al.A simple method for biocatalyst immobilization using PVA-based hydrogel particles[J].Journal of Chemical Technology and Biotechnology,2009,84:561. |
| [15] | Kuyukina, MS;Ivshina, IB;Gavrin, AY;Podorozhko, EA;Lozinsky, VI;Jeffree, CE;Philp, JC .Immobilization of hydrocarbon-oxidizing bacteria in poly(vinyl alcohol) cryogels hydrophobized using a biosurfactant[J].Journal of Microbiological Methods,2006(3):596-603. |
| [16] | Antczak M S et al.Stability of extracellular proteinase productivity by Bacillus subtilis cells immobilized in PVA-cryogel[J].Enzyme and Microbial Technology,2004,34:168. |
| [17] | Doretti L;Ferrara D et al.PEG-modified glucose oxidase immobilized on a PVA cryogel membrane for amperometric biosensor applications[J].Talanta,1998,45:891. |
| [18] | Bacheva,AV;Plieva,FM;Lysogorskaya,EN;,IYu;Lozinsky,VI .Peptide synthesis in organic media with subtilisin 72 immobilized on poly(vinyl alcohol)-cryogel carrier.[J].Bioorganic and Medicinal Chemistry Letters,2001(8):1005-1008. |
| [19] | Busto M D;Meza V;Ortega N et al.Immobilization of naringinase from Aspergillus niger CECT 2088 in poly(vinyl alcohol)cryogels for the debittering of juices[J].Food Chemistry,2007,104:1177. |
| [20] | Santos, JC;Paula, AV;Rocha, CGF;Nunes, GFM;de Castro, HF .Morphological and mechanical properties of hybrid matrices of polysiloxane-polyvinyl alcohol prepared by sol-gel technique and their potential for immobilizing enzyme[J].Journal of Non-Crystalline Solids,2008(42/44):4823-4826. |
| [21] | Santos JC;Mijone PD;Nunes GFM;Perez VH;de Castro HF .Covalent attachment of Candida rugosa lipase on chemically modified hybrid matrix of polysiloxane-polyvinyl alcohol with different activating compounds[J].Colloids and Surfaces, B. Biointerfaces,2008(2):229-236. |
| [22] | Neff D F M;Balcao V M;Carneiro-da-Cunha M G et al.Immobilization of β-galactosidase from Kluyveromyces lactis onto a polysioxane-polyvinyl alcohol magnetic(mPOS-PVA)composite for lactose hydrolysis[J].Catalysis Communications,2008,9:2334. |
| [23] | Bruno L M;Coelho J S;Melo E H M;Lima Filho J L .Characterization of Mucor miehei lipase immobilized on polysiloxane-polyvinyl alcohol magnetic particles.[J].World Journal of Microbiology & Biotechnology,2005(2):189-192. |
| [24] | Santos J C;Paula A V;Nunes G F M et al.Pseudomonas fluorescens lipase immobilization on polysioxane-polyvinyl alcohol composite chemically modified with epichlorohydrin[J].Journal of Molecular Catalysis B:Enzymatic,2008,52-53:49. |
| [25] | Vecchia R D;Sebrao D et al.Carboxymethylcellulose and poly(vinyl alcohol)used as a film support for lipases immobilization[J].Process Biochemistry,2005,40:2677. |
| [26] | 杨超英,陶玉贵,王强,汤斌.PVA/TiO2杂化膜制备及其固定化酶稳定性的研究[J].安徽工程科技学院学报（自然科学版）,2008(02):7-10. |
| [27] | Hong J;Moosavi-Movahedi A A et al.Direct electron transfer of horseradish peroxidase on Nafion-cysteine modified gold electrode[J].Electrochimica Acta,2007,52:6261. |
| [28] | Kafi A K M;Lee D Y et al.Amperometric biosensor based on direct electrochemistry of hemoglobin in poly-allylamine(PAA)film[J].Thin Solid Films,2007,515:5179. |
| [29] | Yin Y;Lu Y;Wu P et al.Direct electrochemistry of redox proteins and enzymes promoted by carbon nanotubes[J].Sensors,2005,5:220. |
| [30] | Yin YJ;Wu P;Lu YF;Du P;Shi YM;Cai CX .Immobilization and direct electrochemistry of cytochrome c at a single-walled carbon nanotube-modified electrode[J].Journal of solid state electrochemistry,2007(3):390-397. |
| [31] | Xu SY;Peng B;Han XZ .A third-generation H2O2 biosensor based on horseradish peroxidase-labeled Au nanoparticles self-assembled to hollow porous polymeric nanopheres[J].Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices,2007(8):1807-1810. |
| [32] | Zong S;Cao Y;Zhou Y et al.Zirconia nanoparticles enhanced grafted collagen tri-helix scaffold for unmediated biosensing of hydrogen peroxide[J].Langmuir,2006,22:8915. |
| [33] | Zuo SH;Teng YJ;Yuan HH;Lan MB .Direct electrochemistry of glucose oxidase on screen-printed electrodes through one-step enzyme immobilization process with silica sol-gel/polyvinyl alcohol hybrid film[J].Sensors and Actuators, B. Chemical,2008(2):555-560. |
| [34] | Ren G L;Xu X H;Liu Q et al.Electroaspun poly(vinyl alcohol)/glucose oxidase biocomposite membranes for biosensor applications[J].Reactive and Functional Polymers,2006,66:1559. |
| [35] | Wu LL;Yuan XY;Sheng J .Immobilization of cellulase in nanofibrous PVA membranes by electrospinning[J].Journal of Membrane Science,2005(1/2):167-173. |
| [36] | Wang Y;Hsieh YL .Immobilization of lipase enzyme in polyvinyl alcohol (PVA) nanofibrous membranes[J].Journal of Membrane Science,2008(1/2):73-81. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%