研究了RADA16-I纳米短肽的二级结构和微观形态及其在不同溶液触发下形成的水凝胶的力学强度.试验结果表明,该短肽在水溶液中形成典型的β-sheet结构,这对其形成纳米纤维十分重要.此外,研究发现采用不同的溶液触发得到的水凝胶的力学强度可在一定范围内变化,符合DLVO理论.这可为组织工程提供强度可调控的纳米纤维支架材料.
参考文献
[1] | Zhang SG .Beyond the Petri dish[J].Nature biotechnology,2004(2):151-152. |
[2] | Zhang S;Gelain F;Zhao X .Designer self-assembling peptide nanofiber scaffolds for 3D tissue cell cultures.[J].Seminars in cancer biology,2005(5):413-420. |
[3] | Zhang S;Altman M .Peptide self-assembly in functional polymer science and engineering[J].Reactive and Functional Polymers,1999,41(1-3):91. |
[4] | Zhang S .Fabrication of novel biomaterials through molecular self-assembly.[J].Nature biotechnology,2003(10):1171-1178. |
[5] | Hartgerink J D;Beniash E;Stupp S I .Self-assembly and mineralization of peptide-amphiphile nanofibers[J].Science,2001,294(5547):1684. |
[6] | AGGELI A.;BELL M.;BODEN N.;KEEN J. N.;KNOWLES P. F.;MCLEISH T. C. B.;PITKEATHLY M.;RADFORD S. E. .RESPONSIVE GELS FORMED BY THE SPONTANEOUS SELF-ASSEMBLY OF PEPTIDES INTO POLYMERIC BETA-SHEET TAPES[J].Nature,1997(6622):259-262. |
[7] | Yokoi H;Kinoshita T;Zhang SG .Dynamic reassembly of peptide RADA16 nanofiber scaffold[J].Proceedings of the National Academy of Sciences of the United States of America,2005(24):8414-8419. |
[8] | Semino CE;Kasahara J;Hayashi Y;Zhang S .Entrapment of migrating hippocampal neural cells in three-dimensional peptide nanofiber scaffold.[J].Tissue engineering,2004(3/4):643-655. |
[9] | Semino CE;Merok JR;Crane GG;Panagiotakos G;Zhang S .Functional differentiation of hepatocyte-like spheroid structures from putative liver progenitor cells in three-dimensional peptide scaffolds.[J].Differentiation: The Journal of the International Society of Differentiation,2003(4/5):262-270. |
[10] | Ye Z;Zhang H;Luo H et al.Temperature and pH effects on biophysical and morphological properties of self-assembling peptide RADA16-I[J].Journal of Peptide Science,2008,14(02):152. |
[11] | Schneider JP.;Pochan DJ.;Ozbas B.;Rajagopal K.;Pakstis L.;Kretsinger J. .Responsive hydrogels from the intramolecular folding and self-assembly of a designed peptide[J].Journal of the American Chemical Society,2002(50):15030-15037. |
[12] | Finkelstein A V;Ptitsyn O.Protein physics:A course of lectures[M].San Diego:academic Press,2002 |
[13] | Caplan M R et al.Self-assembly of a beta-sheet protein governed by relief of electrostatic repulsion relative to van der Waals attraction[J].Biomacromolecules,2000,1(04):627. |
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