用红外光谱分析了重组胶原蛋白和牛源I型胶原蛋白的结构,并用场发射扫描电镜观察了两种胶原蛋白的形貌.结果表明,两种胶原蛋白的红外光谱存在显著差异,牛源I型胶原蛋白的红外光谱呈典型的三螺旋结构特征,而重组胶原蛋白的红外光谱呈变性胶原蛋白特征;重组胶原蛋白的红外光谱在1024 cm-1附近有很强的丝氨酸侧基C-O伸缩振动吸收峰;牛源I型胶原蛋白分子形成胶原纤维,重组胶原蛋白没有纤维存在,重组胶原蛋白与牛源I型胶原蛋白的分子构型和聚集结构不同.研究结果将为胶原基生物医学材料和组织工程支架的设计使用提供理论依据.
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