采用纳米银-壳聚糖复合膜固定葡萄糖氧化酶,构建葡萄糖生物传感器.利用计时电流法对不同光照时间纳米银颗粒组装的酶电极响应电流进行了表征.实验结果表明,光照纳米银颗粒可以抑制葡萄糖生物传感器的响应电流;随着光照时间的延长,纳米银颗粒的抑制作用逐渐增强,当光照时间达到120 min时,葡萄糖生物传感器的响应电流最小(-3.953 μA/cm~2).葡萄糖生物传感器响应电流的抑制可能是由纳米银颗粒表面的Ag~+离子浓度及表面性能的变化引起的.
The silver nanoparticle/chitosan composite matrix was developed to fabricate the glucose biosensor. The response current of the enzyme electrode containing AgNPs photo-induced at differ-ent time was characterized by chronoamperometry. The experimental results indicated that the re-sponse current of the glucose biosensor was inhibited by the photo-induced silver nanoparticles. The inhibitory effect of silver nanoparticles enhanced along with the photo-induced time. When the pho-to-induced time of silver nanoparticles was 120 min, the response current of the glucose biosensor reached the minimum value, -3. 953 μ/cm~2. The inhibition of response current of the glucose biosensor is probably due to the change of Ag~+ ions concentration and the new surface formation of silver nanoparticles.
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