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We have, for the first time, observed directly the Cu-O chains adsorbed on a Cu(110) surface using a high resolution electron microscope (HREM). This observation was confirmed by a multi-slice image simulation based on electron diffraction and imaging theory. Our calculations indicate quantitatively under what conditions an adsorption layer can be observed by HREM with sufficient contrast. This study reveals the potential of HREM to investigate surface structures of a ultra-thin film with thicknesses of nanometers at atomic level.

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