以京尼平(Genipin)为交联剂,通过粒子沥滤结合冷冻干燥工艺制备纳米羟基磷灰石/羧甲基壳聚糖复合支架.然后依据组织工程的原则,将pcDNA3.1-血管内皮生长因子(Vascular endothelial growth factor,VEGF)165质粒转染后的骨髓间充质干细胞(Bone marrow stromal cells,BMSCs)与支架复合构建组织工程骨.最后将该组织工程骨植入到兔桡侧,观察其成骨能力及降解速度.结果表明:京尼平交联的支架材料的微观结构、力学性能与天然松质骨相似,可满足支架材料的要求；且支架材料具有自发荧光特征,便于观察支架的微结构及界面上细胞的粘附情况；动物实验表明所得复合骨具有生物相容性好、无毒副作用的优点及促进局部微血管形成、加快骨缺损修复的作用,其降解速度与骨生长速度基本匹配,是一种潜在的性能优良的骨修复材料.
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