利用二甘醇作溶剂和还原剂水解还原三氯化铁,合成水溶性的葡聚糖包被磁性纳米材料(MNPs),选择大鼠的肝脏细胞BRL3A、肾脏细胞NRK、星型角质细胞和外周血单个核细胞(PBMC),运用流式细胞仪(FCM)检测细胞凋亡和苏木素伊红(HE)染色细胞形态学观察进行细胞毒性研究.结果发现,MNPs共培养浓度达到64μg/ml时,PBMC出现明显凋亡(p＜0.01),而其它几种细胞在MNPs共培养浓度达到128μg/ml时,仍未出现明显凋亡(p＞0.05).电镜观察发现BRL3A、NRK和星型角质细胞均出现内吞该纳米颗粒,而未观察到PBMC的吞噬现象.结果提示,葡聚糖包被的MNPs的细胞毒性具有细胞特异性,实质性脏器细胞毒性较小,可能与其存在内吞MNPs能力有关,提示葡聚糖包被的MNPs可以运用于机体实质脏器核磁造影剂和药物载体.
参考文献
| [1] | Pankhurst Q A;Connolly J;Jones S K et al.[J].Journal of Physics D:Applied Physics,2003,36(13):R167-R181. |
| [2] | Gupta AK;Gupta M .Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications[J].Biomaterials,2005(18):3995-4021. |
| [3] | Soto K;Garza KM;Murr LE .Cytotoxic effects of aggregated nanomaterials.[J].Acta biomaterialia,2007(3):351-358. |
| [4] | Lewinski N;Colvin V;Drezek R .Cytotoxicity of nanoparticles[J].Small,2008(1):26-49. |
| [5] | Gupta AK;Wells S .[J].IEEE TRANSACTIONS IN NANOBIOSCIENCE,2004,3(01):66-73. |
| [6] | Gupta AK;Gupta M .Cytotoxicity suppression and cellular uptake enhancement of surface modified magnetic nanoparticles.[J].Biomaterials,2005(13):1565-1573. |
| [7] | Hu F;Neoh K;Cen L et al.[J].Biomacromolecules,2006,7(03):809-816. |
| [8] | Petri Fink A;Chastellain M;Juillerat Jeanneret L;Ferrari A;Hofmann H .Development of functionalized superparamagnetic iron oxide nanoparticles for interaction with human cancer cells.[J].Biomaterials,2005(15):2685-2694. |
| [9] | Cengelli F;Maysinger D;Tschudi-Monnet F et al.[J].Journal of Pharmacology and Experimental Therapeutics,2006,318(01):108-116. |
| [10] | Jordan A;Scholz R;Wust P et al.[J].Journal of Magnetism and Magnetic Materials,1999,194(1-3):185-196. |
| [11] | William W Yu;Emmanuel Chang;Christie M Sayes .Aqueous dispersion of monodisperse magnetic iron oxide nanocrystals through phase transfer[J].Nanotechnology,2006(17):4483-4487. |
| [12] | Muller K;Skepper JN;Posfai M;Trivedi R;Howarth S;Corot C;Lancelot E;Thompson PW;Brown AP;Gillard JH .Effect of ultrasmall superparamagnetic iron oxide nanoparticles (Ferumoxtran-10) on human monocyie-macrophages in vitro[J].Biomaterials,2007(9):1629-1642. |
| [13] | Cheng FY;Su CH;Yang YS;Yeh CS;Tsai CY;Wu CL;Wu MT;Shieh DB .Characterization of aqueous dispersions of Fe(3)O(4) nanoparticles and their biomedical applications.[J].Biomaterials,2005(7):729-738. |
| [14] | Ito A;Ino K;Kobayashi T;Honda H .The effect of RGD peptide-conjugated magnetite cationic liposomes on cell growth and cell sheet harvesting[J].Biomaterials,2005(31):6185-6193. |
| [15] | Zhang Y;Kohler N;Zhang M .Surface modification of superparamagnetic magnetite nanoparticles and their intracellular uptake.[J].Biomaterials,2002(7):1553-1561. |
| [16] | Lee H;Lee E;Kim DK;Jang NK;Jeong YY;Jon S .Antibiofouling polymer-coated superparamagnetic iron oxide nanoparticles as potential magnetic resonance contrast agents for in vivo cancer imaging[J].Journal of the American Chemical Society,2006(22):7383-7389. |
| [17] | Thoren L .[J].Developments in Biological Standardization,1980,48:157-167. |
| [18] | Mehvar R. .Dextrans for targeted and sustained delivery of therapeutic and imaging agents [Review][J].Journal of Controlled Release: Official Journal of the Controlled Release Society,2000(1):1-25. |
| [19] | Jung C W .[J].Magn Reson Image,1995,13(05):675-691. |
| [20] | Tao K;Dou HJ;Sun K .Combined investigation of experimental characterization and theoretic calculation on the structure of dextran-Fe3O4 clusters[J].Colloids and Surfaces, A. Physicochemical and Engineering Aspects,2006(1/3):70-76. |
| [21] | Bourlinos AB.;Bakandritsos A.;Georgakilas V.;Petridis D. .Surface modification of ultrafine magnetic iron oxide particles[J].Chemistry of Materials,2002(8):3226-0. |
| [22] | Ge J;Hu Y;Biasini M et al.[J].Chem Euro J,2007,13(25):7153-7161. |
| [23] | Hussain SM;Hess KL;Gearhart JM;Geiss KT;Schlager JJ .In vitro toxicity of nanoparticles in BRL 3A rat liver cells.[J].Toxicology in vitro: an international journal published in association with BIBRA,2005(7):975-983. |
| [24] | Fleige G;Nolte C;Synowitz M et al.[J].Neoplasia,2001,3(06):489-499. |
| [25] | Metz S;Bonaterra G;Rudelius M;Settles M;Rummeny EJ;Daldrup Link HE .Capacity of human monocytes to phagocytose approved iron oxide MR contrast agents in vitro.[J].European radiology,2004(10):1851-1858. |
| [26] | Arbab A S;Bashaw LA;Miller B R et al.[J].Transplantation,2003,76(07):1123-1130. |
| [27] | Brunner T;Wick P;Manser P et al.[J].Environmental Science and Technology,2006,40(14):4374-44381. |
| [28] | Shin SH;Ye MK;Kim HS;Kang HS .The effects of nano-silver on the proliferation and cytokine expression by peripheral blood mononuclear cells.[J].International immunopharmacology,2007(13):1813-1818. |
| [29] | Kohler N;Sun C;Wang J;Zhang MQ .Methotrexate-modified superparamagnetic nanoparticles and their intracellular uptake into human cancer cells[J].Langmuir: The ACS Journal of Surfaces and Colloids,2005(19):8858-8864. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%