We reported recombinant human-like type I collagen inducing growth of hydroxyapatite crystals in vitro in the form of self-assembly of nano-fibrils of mineralized collagen resembling extracellular matrix, which obey the same rules, but is superior to the collagen derived from animal tissues because the latter may carry diseases of animals and cause immunological reactions. The mineralized collagen fibrils aligned parallel to each other to form mineralized collagen fibers. Hydroxyapatite nanocrystals grew on the surface of these collagen fibrils with the c-axis of nanocrystals of HA orienting along the longitudinal axis of the fibrils.
参考文献
| [1] | S.Weiner;H.D.Wagner .The material bone: structure-Mechanical function relations[J].Annual review of materials research,1998(0):271-298. |
| [2] | Wen HB.;Zhu XD.;Cui FZ. .MICROSTRUCTURAL FEATURES OF NON-UNION OF HUMAN HUMERAL SHAFT FRACTURE[J].Journal of Structural Biology,1997(3):239-246. |
| [3] | S I Stupp;G C Mejicano;J A Hanson .Organoapatites:Materials for Artificial Bone:Hardening Reactions and Properties[J].Journal of Biomedical Materials Research Part A,1993,27(03):289-299. |
| [4] | Y Doi;T Horiguchi;Y Moriwaki;H Kitago T Kajimoto,Y Iwayama .Formation of Apatite-collagen Complexes[J].Journal of Biomedical Materials Research,1996,31:43-49. |
| [5] | R. Z. Wang;F. Z. Cui;H. B. Lu;H. B. Wen;C. L. Ma;H. D. Li .Synthesis of nanophase hydroxyapatite/collagen composite[J].Journal of Materials Science Letters,1995(7):490-492. |
| [6] | C Du;F Z Cui;W Zhang;Q L Feng X D Zhu,K de Groot .Formation of Calcium Phosphate/Collagen Composites through Mineralization of Collagen Matrix[J].Journal of Biomedical Materials Research Part A,2000,50(04):518-27. |
| [7] | C Combes;C Rey;M Freche .In Vitro Crystallization of Octacalcium Phosphate on Type I Collagen:Influence of Serum Albumin[J].Journal of Materials Science:Materials in Medicine,1999,10(03):153-60. |
| [8] | K S Tenhuisen;R I Martin;M Klimkiewicz;P W Brown .Formation and Properties of a Synthetic Bone Composite:Hydroxyapatite-Collagen[J].Journal of Biomedical Materials Research Part A,1995,29(07):803-10. |
| [9] | K I Clarke;S E Graves;A T C Wong;J T Triffitt M J O Francis,J T Czemuszka .Investigation into the Formation and Mechanical Properties of a Bioactive Material Based on Collagen and Calcium Phosphate[J].Journal of Materials Science:Materials in Medicine,1993,4(02):107-10. |
| [10] | J H Bradt;M Mertig;A Teresiak;W Pompe .Biomimetic Mineralization of Collagen by Combined Fibril Assembly and Calcium Phosphate Formation[J].CHEMISTRY OF MATERIALS,1999,11(10):2694-701. |
| [11] | W Traub;T Arad;S Weiner .3 Dimensional Ordered Distribution of Crystals in Turkey Tendon Collagen-fibers[J].Proceedings of the National Academy of Sciences of the United States of America,1989,86(24):9822-26. |
| [12] | Jie Song;Viengkham Malathong;Carolyn R.Bertozzi .Mineralization of Synthetic Polymer Scaffolds:A Bottom-Up Approach for the Development of Artificial Bone[J].Journal of the American Chemical Society,2005(10):3366-3372. |
| [13] | J C Brown;R Timpl .The Collagen Superfamily[J].International Archives of Allergy and Applied Immunology,1995,107(04):484-90. |
| [14] | Nokelainen M;Tu HM;Vuorela A;Notbohm H;Kivirikko KI;Myllyharju .High-level production of human type I collagen in the yeast Pichiapastoris[J].Yeast,2001(9):797-806. |
| [15] | D D Fan .Synthesis Method of one Human-like Collagen Protein[P].CN 011067578,2002-10-02. |
| [16] | Y Zhai;F Z Cui;Y Wang .Formation of Nano-hydroxyapatite on Recombinant Human-like Collagen Fibrils[J].Current Applied Physics,2005,5(05):429-432. |
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