采用新型水热氧化合成方法制备了纤维素基质固定化乙酰胆碱酯酶载体材料——双醛纤维素(DAC).通过红外光谱(IR)、核磁共振(NMR)、X射线衍射(XRD)和扫描电镜(SEM)对产物进行了表征.测试了DAC固载乙酰胆碱酯酶(AChE)的酶学性能,并与交联壳聚糖(CCTS)、双醛淀粉(DAS)固载AChE的酶学性能进行比较分析,构建了酶学性能理论方程.结果表明,新型水热氧化反应制得了高醛基含量的DAC;DAC固定化AChE与CCTS和DAS固定化AChE相比,稳定性好(10次重复利用率),可在25℃～55℃和pH 4.0～8.0范围保持酶活性;建立的理论方程较好地表征了固载酶的酶学性能.
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