Ti-SBA-15介孔材料用于磷酸化肽的高效富集

色谱 , 2010, 28(2): 123-127.

Ti-SBA-15介孔材料用于磷酸化肽的高效富集

张宇 ^1, , 秦洪强 ^2, , 吴仁安 ^3, , 邹汉法 ^4,

1.中国科学院大连化学物理研究所,中国科学院分离分析化学重点实验室,辽宁,大连,116023

2.中国科学院大连化学物理研究所,中国科学院分离分析化学重点实验室,辽宁,大连,116023

3.中国科学院大连化学物理研究所,中国科学院分离分析化学重点实验室,辽宁,大连,116023

4.中国科学院大连化学物理研究所,中国科学院分离分析化学重点实验室,辽宁,大连,116023

基金项目: 国家自然科学基金(20735004)

关键词： Ti-SBA-15介孔材料 , 磷酸化肽 , 选择性富集 , 基质辅助激光解吸飞行时间质谱

Applications of Ti-SBA-15 mesoporous material in high performance enrichment of phosphopeptides

Keywords： titanium-incorporated SBA-15 mesoporous material (Ti-SBA-15) , phosphopeptides , selective enrichment , matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS)

结合基质辅助激光解吸飞行时间质谱(MALDI-TOF MS)检测技术,考察了Ti-SBA-15介孔材料对β-酪蛋白酶解产物中磷酸化肽的选择性富集性能.实验结果显示,含Ti和Si物质的量比为0.08的Ti-SBA-15介孔材料可选择性地对β-酪蛋白酶解产物中的磷酸化肽进行选择性富集;对于β-酪蛋白和牛血清白蛋白物质的量比为1∶100的蛋白质酶解混合液,Ti-SBA-15仍能实现对其磷酸化肽的有效富集.上述结果表明,作为一种多孔、高比表面积的磷酸化多肽的选择性吸附材料,Ti-SBA-15有望在磷酸化蛋白质组的分析中得到广泛的应用.

A titanium-incorporated SBA-15mesoporous material (Ti-SBA-15) was synthesized via the co-condensation of tetraethyl orthosilicate (TEOS) and tetrabutyl titanate using surfactant P123 as the template.Due to the existence of Ti in the framework of SBA-15,the synthesized Ti-SBA-15 was applied as the selective adsorbent of phosphopeptides from the complex tryptic digest of β-casein.The matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) analysis showed that the phosphopeptides of β-casein digest could be selectively enriched by the Ti-SBA-15 with Ti/Si molar ratio of 0.08,even under the interference of bovine serum albumin (BSA) with the molar ratio of β-casein to BSA up to 1∶100.It can be concluded that the Ti-SBA-15 showed the specific adsorption toward the phosphorylated peptides,which provides great potential in the specific capture of phosphopeptides.

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