采用分子印迹技术,以Ala和Phe为模板分子,苯乙烯和甲基丙烯酸为共聚单体,二乙烯苯为交联剂,Fe3O4为磁性载体,制备了对两种氨基酸有特异性识别的磁性分子印迹聚合物Ala-MMIPs和Phe-MMIPs。研究了两种印迹聚合物的吸附特性,并探讨了吸附机理。结果表明,Phe在其印迹聚合物上的最大吸附量和吸附平衡常数分别为231.62μmol/g和0.21L/mmol;而Ala在它的印迹微球上的最大吸附量和吸附平衡常数分别为179.16μmol/g和0.12L/mmol。说明Phe与Phe-MMIPs有3个作用位点,Ala与Ala-MMIPs之间有2个作用位点,因此吸附能力前者大于后者。
Two amino acids magnetic molecularly imprinted polymers, Ala-MMIPs and Phe-MMIPs were prepared by using Ata and Phe as template molecules, styrene and methacrylic acid as functional monomers, divinylbenzene as cross-linking agent and Fe3O4 as magnetic fluid. Adsorption properties of these two imprinted polymers were researched. Adsorption mechanism was also discussed. The maximum adsorption capacity (qmax) and the adsorption equilibrium constant (K) of Phe on Phe-MMIPs were 231. 62μmol/g and 0. 21L/ mmol respectively. The maximum adsorption capacity and the adsorption equilibrium constant of Ala on AlaMMIPs were 179. 16μmol/g and 0. 12L/mmol respectively. The results exhibit that three site interaction between Phe and Phe-MMIPs was stronger than the two-site interaction between Ala and Ala-MMIPs.
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