利用溶菌酶对中等脱乙酰度壳聚糖的选择性降解,将其与高脱乙酰度壳聚糖以不同比例混合浇铸成膜.考察了降解过程中材料的失重和溶液还原糖含量的变化,用傅立叶红外光谱分析了混合膜的成分改变和降解过程中膜的变化,并用扫描电镜观察了混合膜降解后的形态变化.结果表明,壳聚糖混合膜中的中等脱乙酰度壳聚糖被溶菌酶选择性的降解,而且当混合膜中该组分含量为0.5时,降解后膜表面及内部均产生了互相贯穿的纳米尺寸的孔,剩余的高脱乙酰度成分表现为纳米尺寸的纤维.该方法通过壳聚糖的选择性酶解原位成孔,为制备纳米结构的多孔壳聚糖膜提供了一个新颖的途径.
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