吖啶-9-羧酸苯酚酯是一类效率较高的化学发光试剂.其结构一般有两部分组成,吖啶环发光部分和9-羧酸苯酚酯离去基团.本文对离去基团进行修饰,分别在离去基团苯酚部分的2,5或2,6位引入取代基CR、NO2、Br、CH3,合成了4个新吖啶酯衍生物,并对它们的分子结构进行了表征.4个新化合物和模型化合物(无取代基)比较,均表现出较好的化学发光效率;发光动力学基本符合文献报道规律.而且化学发光法对其水解稳定性考察显示,取代基的电性和位阻是影响稳定性的两大因素.
参考文献
| [1] | Dodeigne C. .Review Chemiluminescence as a diagnostic tool. A review[J].Talanta: The International Journal of Pure and Applied Analytical Chemistry,2000(3):415-439. |
| [2] | L.J.Kricka .Clinical applications of chemiluminescence[J].Analytica chimica acta,2003(1/2):279-286. |
| [3] | D. Whitney King;William J. Cooper;Steven A. Rusak;Barrie M. Peake;James J. Kiddle;Daniel W. O'Sullivan;Megan L. Melamed;Chris R. Morgan;Stephen M. Theberge .Flow Injection Analysis of H_(2)O_(2) in Natural Waters Using Acridinium Ester Chemiluminescence: Method Development and Optimization Using a Kinetic Model[J].Analytical chemistry,2007(11):4169-4176. |
| [4] | Kuruvilla E;Ramaiah D .Selective interactions of a few acridinium derivatives with single strand DNA: Study of photophysical and DNA binding interactions[J].The journal of physical chemistry, B. Condensed matter, materials, surfaces, interfaces & biophysical,2007(23):6549-6556. |
| [5] | Natrajan A;Jiang Q;Sherpe D et al.High quantum yield acridinium compounds and their use in improving assay sensitivity[P].WO 2006130736,2006-12-07. |
| [6] | Brown RC.;Fisher M.;Harbron S.;Taylorson CJ.;Woodhead JS.;Weeks I. .Employment of a phenoxy-substituted acridinium ester as a long-lived chemiluminescent indicator of glucose oxidase activity and its application in an alkaline phosphatase amplification cascade immunoassay[J].Analytical Biochemistry,1998(1):142-151. |
| [7] | McCapra F .Chemical mechanisms in bioluminescence[J].Accounts of Chemical Research,1976,9(06):201-208. |
| [8] | Sato N. .SYNTHESIS AND PROPERTIES OF NEW LUMINESCENT 10-CARBOXYMETHYLACRIDINIUM DERIVATIVES[J].Tetrahedron letters: The International Journal for the Rapid Publication of Preliminary Communications in Organic Chemistry,1996(47):8519-8522. |
| [9] | Sato N;Mochizuki H;Kanamori T et al.Acridinium compounds and conjugates thereof[P].US 5594112,1997-01-14. |
| [10] | Smith Keith .Synthesis and properties of novel chemiluminescent biological probes:substituted 4-(2-succinimidyloxycarbonylethyl)phenyl 10-methylacridinium-9-carboxylate trifluoromethanesulphonate[J].Journal of Photochemistry and Photobiology, A. Chemistry,2000(3):181-191. |
| [11] | Denise M W;Burton D J .Pregeneration,spectroscopic detection and chemical reactivity of(trifluoromethyl)copper,an elusive and complex species[J].Journal of the American Chemical Society,1986,108(04):832-834. |
| [12] | Burton D J;Denise M W .A remarkably simple preparation of(trifluoromethyl)cadmium and-zinc reagents directly from difluorodihalomethanes[J].Journal of the American Chemical Society,1985,107(17):5014-5015. |
| [13] | Weeks I;Rutter A;Li Z et al.Chemiluminescent compounds[P].US 2007166759,2007-07-19. |
| [14] | Weeks I;RutterA;Li Z et al.Chemiluminescent acridinium compounds that emit chemiluminescence below pH 12 and materials labelled therewith for use in assays[P].GB 2405407,2005-03-02. |
| [15] | Holland A M .Synthesis of novel chemiluminescemt compounds[D].Swansea:University of wales,2002. |
| [16] | Mu X .Synthesis of novel chemiluminmcem compunds[D].Swansea:University of wales,2005. |
上一张
下一张
上一张
下一张
计量
- 下载量()
- 访问量()
文章评分
- 您的评分：
-
10%
-
20%
-
30%
-
40%
-
50%