采用天然高分子聚合物甲壳素作载体,以戊二醛为交联剂,对蜗牛酶进行固定化.研究了酶量、戊二醛浓度、pH和溶剂对蜗牛酶固定化的影响.结果表明,0.5 g甲壳素与0.8%的戊二醛交联后,与用双蒸水配制的pH为5.6的酶液混合制备的固定化酶活性最高.用优化条件下制备的固定化酶降解壳聚糖,先调pH为4.0,360 min后调pH为4.8使得平均降解速率最大.当壳聚糖质量百分数为0.3%时,初始反应速率最大,并提出了新的经验模型描述壳聚糖的降解过程.用该固定化酶连续降解壳聚糖70 d,酶活为原来的98%,降解性能保持稳定.
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